Browse by MODEL: Hybrid

SASDLP7 – Two-chain hepatocyte growth factor/scatter factor in complex with receptor tyrosine kinase MET

Hepatocyte growth factorHepatocyte growth factor receptor experimental SAS data
SASREF model
Sample: Hepatocyte growth factor monomer, 79 kDa Mus musculus protein
Hepatocyte growth factor receptor monomer, 100 kDa Mus musculus protein
Buffer: 50 mM MES, 150 mM NaCl, pH: 6.7
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2004 Feb 9
Structural basis of hepatocyte growth factor/scatter factor and MET signalling Proceedings of the National Academy of Sciences 103(11):4046-4051 (2006)
Gherardi E, Sandin S, Petoukhov M, Finch J, Youles M, Ofverstedt L, Miguel R, Blundell T, Vande Woude G, Skoglund U, Svergun D
RgGuinier 6.7 nm
Dmax 20.0 nm
VolumePorod 370 nm3

SASDLQ7 – receptor tyrosine kinase MET

Hepatocyte growth factor receptor experimental SAS data
SASREF model
Sample: Hepatocyte growth factor receptor monomer, 100 kDa Mus musculus protein
Buffer: 50 mM MES, 150 mM NaCl, pH: 6.7
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2004 Feb 7
Structural basis of hepatocyte growth factor/scatter factor and MET signalling Proceedings of the National Academy of Sciences 103(11):4046-4051 (2006)
Gherardi E, Sandin S, Petoukhov M, Finch J, Youles M, Ofverstedt L, Miguel R, Blundell T, Vande Woude G, Skoglund U, Svergun D
RgGuinier 4.8 nm
Dmax 16.0 nm
VolumePorod 190 nm3

SASDLQ5 – Tricorn protease oligomers

Tricorn protease experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Tricorn protease hexamer, 730 kDa Thermoplasma acidophilum (strain … protein
Buffer: 20mM Tris-HCl, 100 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2003 Jan 14
X-ray snapshots of peptide processing in mutants of tricorn-interacting factor F1 from Thermoplasma acidophilum. J Biol Chem 280(39):33387-96 (2005)
Goettig P, Brandstetter H, Groll M, Göhring W, Konarev PV, Svergun DI, Huber R, Kim JS
RgGuinier 5.9 nm

SASDC52 – Bruton tyrosine kinase

Tyrosine-protein kinase BTK (R28C mutant) experimental SAS data
GASBOR model
Sample: Tyrosine-protein kinase BTK (R28C mutant) monomer, 76 kDa Homo sapiens protein
Buffer: 20 mM HEPES 200 mM NaCl, 2 mM DTT and 1 mM MgCl2, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2002 Apr 2
Conformation of full-length Bruton tyrosine kinase (Btk) from synchrotron X-ray solution scattering. EMBO J 22(18):4616-24 (2003)
Márquez JA, Smith CI, Petoukhov MV, Lo Surdo P, Mattsson PT, Knekt M, Westlund A, Scheffzek K, Saraste M, Svergun DI
RgGuinier 5.0 nm
Dmax 20.0 nm
VolumePorod 130 nm3

SASDLN7 – 1:1 complex of Ferredoxin-dependent glutamate synthase 2 (FdGlts) with ferredoxin

Ferredoxin-dependent glutamate synthase 2Ferredoxin-1 experimental SAS data
OTHER model
Sample: Ferredoxin-dependent glutamate synthase 2 monomer, 169 kDa Synechocystis sp. (strain … protein
Ferredoxin-1 monomer, 11 kDa Nostoc sp. (strain … protein
Buffer: Hepes– KOH buffer, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2002 Jun 4
The Active Conformation of Glutamate Synthase and its Binding to Ferredoxin Journal of Molecular Biology 330(1):113-128 (2003)
van den Heuvel R, Svergun D, Petoukhov M, Coda A, Curti B, Ravasio S, Vanoni M, Mattevi A
RgGuinier 3.6 nm
Dmax 12.3 nm
VolumePorod 262 nm3

SASDAX2 – Pyruvate decarboxylase (PDC) from Z. mobilis

Pyruvate decarboxylase experimental SAS data
CRYSOL model
Sample: Pyruvate decarboxylase tetramer, 244 kDa Zymomonas mobilis protein
Buffer: 100 mM Sodium Citrate, 17% Glycerol, 22.5% PEG 1500, pH: 6
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 1998 Nov 3
Crystal versus solution structures of thiamine diphosphate-dependent enzymes. J Biol Chem 275(1):297-302 (2000)
Svergun DI, Petoukhov MV, Koch MH, König S
RgGuinier 3.9 nm
Dmax 11.0 nm