Small Angle Scattering Biological Data Bank SASBDB

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SASDRE3 – Tissue Transglutaminase + Ca: Time-resolved 0ms

Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data

Protein-glutamine gamma-glutamyltransferase 2 Kratky plot

Sample:	Protein-glutamine gamma-glutamyltransferase 2, 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2021 Nov 19

Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
Zielinski K, Katz A, Calvey G, Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L

R_g^Guinier	4.1	nm
D_max	16.0	nm
Volume^Porod	150	nm³

SASDRF3 – Tissue Transglutaminase + Ca: Time-resolved 32 ms

Sample:	Protein-glutamine gamma-glutamyltransferase 2, 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2021 Nov 19

R_g^Guinier	4.1	nm
D_max	17.0	nm
Volume^Porod	170	nm³

SASDRG3 – Tissue Transglutaminase + Ca: Time-resolved 63 ms

Sample:	Protein-glutamine gamma-glutamyltransferase 2, 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2021 Nov 19

R_g^Guinier	4.1	nm
D_max	18.0	nm
Volume^Porod	155	nm³

SASDRH3 – Tissue Transglutaminase + Ca: Time-resolved 100 ms

Sample:	Protein-glutamine gamma-glutamyltransferase 2, 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2021 Nov 19

R_g^Guinier	4.1	nm
D_max	20.0	nm
Volume^Porod	190	nm³

SASDRJ3 – Tissue Transglutaminase + Ca: Time-resolved 316 ms

Sample:	Protein-glutamine gamma-glutamyltransferase 2, 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2021 Nov 19

R_g^Guinier	4.5	nm
D_max	24.0	nm
Volume^Porod	200	nm³

SASDRK3 – Tissue Transglutaminase + Ca: Time-resolved 631 ms

Sample:	Protein-glutamine gamma-glutamyltransferase 2, 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2021 Nov 19

R_g^Guinier	4.6	nm
D_max	26.0	nm
Volume^Porod	215	nm³

SASDRL3 – Tissue Transglutaminase + Ca: Time-resolved 1500 ms

Sample:	Protein-glutamine gamma-glutamyltransferase 2, 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2021 Nov 19

R_g^Guinier	4.6	nm
D_max	28.0	nm
Volume^Porod	230	nm³

SASDRM3 – Tissue Transglutaminase + Ca: 5min Equilibrium

Sample:	Protein-glutamine gamma-glutamyltransferase 2, 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2021 Sep 29

R_g^Guinier	5.0	nm
D_max	27.0	nm
Volume^Porod	241	nm³

SASDRN3 – Tissue Transglutaminase + Ca: 10min Equilibrium

Sample:	Protein-glutamine gamma-glutamyltransferase 2, 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2021 Nov 19

R_g^Guinier	5.5	nm
D_max	26.0	nm
Volume^Porod	300	nm³

SASDRP3 – Tissue Transglutaminase + Ca: 30min Equilibrium

Sample:	Protein-glutamine gamma-glutamyltransferase 2, 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2021 Sep 29

R_g^Guinier	6.9	nm
D_max	31.5	nm
Volume^Porod	490	nm³

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