Small Angle Scattering Biological Data Bank SASBDB

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SASDW75 – Shewanella benthica hemoglobin Y34F 150 MPa

Group 1 truncated hemoglobin (Y34F, C51S, C71S) experimental SAS data

Group 1 truncated hemoglobin (Y34F, C51S, C71S) Kratky plot

Sample:	Group 1 truncated hemoglobin (Y34F, C51S, C71S), 13 kDa Shewanella benthica KT99 protein
Buffer:	14 mM Tris, 6 mM potassium phosphate, pH: 7
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Feb 18

Extremophilic hemoglobins: The structure of Shewanella benthica truncated hemoglobin N Journal of Biological Chemistry :108223 (2025)
Martinez Grundman J, Schultz T, Schlessman J, Johnson E, Gillilan R, Lecomte J

R_g^Guinier	2.2	nm
D_max	7.0	nm
Volume^Porod	61	nm³

SASDW85 – Shewanella benthica hemoglobin Y34F 200 MPa

Sample:	Group 1 truncated hemoglobin (Y34F, C51S, C71S), 13 kDa Shewanella benthica KT99 protein
Buffer:	14 mM Tris, 6 mM potassium phosphate, pH: 7
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Feb 18

R_g^Guinier	2.0	nm
D_max	8.5	nm
Volume^Porod	24	nm³

SASDW95 – Shewanella benthica hemoglobin Y34F 250 MPa

Sample:	Group 1 truncated hemoglobin (Y34F, C51S, C71S), 13 kDa Shewanella benthica KT99 protein
Buffer:	14 mM Tris, 6 mM potassium phosphate, pH: 7
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Feb 18

R_g^Guinier	2.1	nm
D_max	6.6	nm
Volume^Porod	49	nm³

SASDWA5 – Shewanella benthica hemoglobin Y108A 0.101 MPa

Group 1 truncated hemoglobin (C51S, C71S, Y108A) experimental SAS data

Group 1 truncated hemoglobin (C51S, C71S, Y108A) Kratky plot

Sample:	Group 1 truncated hemoglobin (C51S, C71S, Y108A), 13 kDa Shewanella benthica KT99 protein
Buffer:	14 mM Tris, 6 mM potassium phosphate, pH: 7
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Feb 18

R_g^Guinier	1.4	nm
D_max	4.0	nm
Volume^Porod	20	nm³

SASDWB5 – Shewanella benthica hemoglobin Y108A 0.101 MPa (replicate)

Sample:	Group 1 truncated hemoglobin (C51S, C71S, Y108A), 13 kDa Shewanella benthica KT99 protein
Buffer:	14 mM Tris, 6 mM potassium phosphate, pH: 7
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Feb 18

R_g^Guinier	1.4	nm
D_max	4.0	nm
Volume^Porod	20	nm³

SASDWC5 – Shewanella benthica hemoglobin Y108A 50 MPa

Sample:	Group 1 truncated hemoglobin (C51S, C71S, Y108A), 13 kDa Shewanella benthica KT99 protein
Buffer:	14 mM Tris, 6 mM potassium phosphate, pH: 7
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Feb 18

R_g^Guinier	1.5	nm
D_max	4.5	nm
Volume^Porod	20	nm³

SASDWD5 – Shewanella benthica hemoglobin Y108A 100 MPa

Sample:	Group 1 truncated hemoglobin (C51S, C71S, Y108A), 13 kDa Shewanella benthica KT99 protein
Buffer:	14 mM Tris, 6 mM potassium phosphate, pH: 7
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Feb 18

R_g^Guinier	1.5	nm
D_max	4.8	nm
Volume^Porod	20	nm³

SASDWE5 – Shewanella benthica hemoglobin Y108A 150 MPa

Sample:	Group 1 truncated hemoglobin (C51S, C71S, Y108A), 13 kDa Shewanella benthica KT99 protein
Buffer:	14 mM Tris, 6 mM potassium phosphate, pH: 7
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Feb 18

R_g^Guinier	1.6	nm
D_max	6.0	nm
Volume^Porod	21	nm³

SASDWF5 – Shewanella benthica hemoglobin Y108A 200 MPa

Sample:	Group 1 truncated hemoglobin (C51S, C71S, Y108A), 13 kDa Shewanella benthica KT99 protein
Buffer:	14 mM Tris, 6 mM potassium phosphate, pH: 7
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Feb 18

R_g^Guinier	1.8	nm
D_max	8.8	nm
Volume^Porod	24	nm³

SASDWG5 – Shewanella benthica hemoglobin Y108A 250 MPa

Sample:	Group 1 truncated hemoglobin (C51S, C71S, Y108A), 13 kDa Shewanella benthica KT99 protein
Buffer:	14 mM Tris, 6 mM potassium phosphate, pH: 7
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Feb 18

R_g^Guinier	2.1	nm
D_max	9.5	nm
Volume^Porod	25	nm³

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