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SASDUN3 – Colletotrichum higginsianum Auxiliary Activity Family 9B Lytic Polysaccharide Monooxygenase (AA9B LPMO) monomer

Endoglucanase-4 experimental SAS data
Endoglucanase-4 Kratky plot
Sample: Endoglucanase-4 monomer, 33 kDa Colletotrichum higginsianum (strain … protein
Buffer: 50 mM MES, 150 mM NaCl, pH: 6.5
Experiment: SAXS data collected at SWING, SOLEIL on 2022 Feb 17
The disordered C-terminal tail of fungal LPMOs from phytopathogens mediates protein dimerization and impacts plant penetration. Proc Natl Acad Sci U S A 121(13):e2319998121 (2024)
Tamburrini KC, Kodama S, Grisel S, Haon M, Nishiuchi T, Bissaro B, Kubo Y, Longhi S, Berrin JG
RgGuinier 3.9 nm
Dmax 17.0 nm
VolumePorod 68 nm3

SASDUP3 – Colletotrichum higginsianum Auxiliary Activity Family 9B Lytic Polysaccharide Monooxygenase (AA9B LPMO) dimer

Endoglucanase-4 experimental SAS data
Endoglucanase-4 Kratky plot
Sample: Endoglucanase-4 dimer, 66 kDa Colletotrichum higginsianum (strain … protein
Buffer: 50 mM MES, 150 mM NaCl, pH: 6.5
Experiment: SAXS data collected at SWING, SOLEIL on 2022 Feb 17
The disordered C-terminal tail of fungal LPMOs from phytopathogens mediates protein dimerization and impacts plant penetration. Proc Natl Acad Sci U S A 121(13):e2319998121 (2024)
Tamburrini KC, Kodama S, Grisel S, Haon M, Nishiuchi T, Bissaro B, Kubo Y, Longhi S, Berrin JG
RgGuinier 6.4 nm
Dmax 27.9 nm
VolumePorod 199 nm3

SASDTV5 – Human full-length Annexin A11 (apo form)

Annexin A11 experimental SAS data
Annexin A11 Kratky plot
Sample: Annexin A11 monomer, 54 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM KCl, 5 mM EDTA, 1 mM DTT, pH: 8.5
Experiment: SAXS data collected at BM29, ESRF on 2023 Jul 21
The structural properties of full-length annexin A11 Frontiers in Molecular Biosciences 11 (2024)
Dudas E, Tully M, Foldes T, Kelly G, Tartaglia G, Pastore A
RgGuinier 3.8 nm
Dmax 15.9 nm
VolumePorod 84 nm3

SASDTW5 – Human full-length Annexin A11 (holo form, with calcium)

Annexin A11 experimental SAS data
Annexin A11 Kratky plot
Sample: Annexin A11 monomer, 54 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM KCl, 500 µM CaCl2, 1 mM DTT, pH: 8.5
Experiment: SAXS data collected at BM29, ESRF on 2023 Jul 21
The structural properties of full-length annexin A11 Frontiers in Molecular Biosciences 11 (2024)
Dudas E, Tully M, Foldes T, Kelly G, Tartaglia G, Pastore A
RgGuinier 3.8 nm
Dmax 17.1 nm
VolumePorod 83 nm3

SASDT35 – N-cadherin extracellular domains EC1-EC5

Cadherin-2 experimental SAS data
Cadherin-2 Kratky plot
Sample: Cadherin-2 dimer, 123 kDa Mus musculus protein
Buffer: 20 mM HEPES, 150 mM NaCl, 3 mM CaCl2, 0.02% NaN3, pH: 7.5
Experiment: SAXS data collected at 13A, Taiwan Photon Source, NSRRC on 2023 May 1
Rapid simulation of glycoprotein structures by grafting and steric exclusion of glycan conformer libraries. Cell 187(5):1296-1311.e26 (2024)
Tsai YX, Chang NE, Reuter K, Chang HT, Yang TJ, von Bülow S, Sehrawat V, Zerrouki N, Tuffery M, Gecht M, Grothaus IL, Colombi Ciacchi L, Wang YS, Hsu MF, Khoo KH, Hummer G, Hsu SD, Hanus C, Sikora M
RgGuinier 8.8 nm
Dmax 40.0 nm
VolumePorod 496 nm3

SASDT45 – N-cadherin extracellular domains EC4-EC5

Cadherin-2 experimental SAS data
Cadherin-2 Kratky plot
Sample: Cadherin-2 monomer, 24 kDa Mus musculus protein
Buffer: 20 mM HEPES, 150 mM NaCl, 3 mM CaCl2, 0.02% NaN3, pH: 7.5
Experiment: SAXS data collected at 13A, Taiwan Photon Source, NSRRC on 2023 May 1
Rapid simulation of glycoprotein structures by grafting and steric exclusion of glycan conformer libraries. Cell 187(5):1296-1311.e26 (2024)
Tsai YX, Chang NE, Reuter K, Chang HT, Yang TJ, von Bülow S, Sehrawat V, Zerrouki N, Tuffery M, Gecht M, Grothaus IL, Colombi Ciacchi L, Wang YS, Hsu MF, Khoo KH, Hummer G, Hsu SD, Hanus C, Sikora M
RgGuinier 3.4 nm
Dmax 12.6 nm
VolumePorod 63 nm3

SASDQJ9 – An assembly between C-terminal truncated Netrin UNC-6 and the extracellular domain of Netrin receptor UNC-5 with porcine intestinal mucosa heparin (UNC-6ΔC-heparin-UNC-5 ECD complex)

Netrin unc-6Isoform a of Netrin receptor unc-5Heparin, porcine intestinal mucosa experimental SAS data
Netrin unc-6 Isoform a of Netrin receptor unc-5 Heparin, porcine intestinal mucosa Kratky plot
Sample: Netrin unc-6 monomer, 52 kDa Caenorhabditis elegans protein
Isoform a of Netrin receptor unc-5 monomer, 38 kDa Caenorhabditis elegans protein
Heparin, porcine intestinal mucosa monomer, 15 kDa Sus scrofa domesticus
Buffer: 10 mM HEPES pH 7.2, 150 mM NaCl, 100 mM MgSO4, pH: 7.2
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jun 18
Structural insights into the formation of repulsive netrin guidance complexes. Sci Adv 10(7):eadj8083 (2024)
Priest JM, Nichols EL, Smock RG, Hopkins JB, Mendoza JL, Meijers R, Shen K, Özkan E
RgGuinier 10.4 nm
Dmax 37.3 nm
VolumePorod 2156 nm3

SASDQK9 – An assembly between C-terminal truncated Netrin UNC-6, the extracellular domains of Netrin receptor UNC-5 and UNC-40 with porcine intestinal mucosa heparin (UNC-6ΔC-heparin-UNC-5 ECD-UNC-40 ECD complex)

Netrin unc-6Isoform a of Netrin receptor unc-5Heparin, porcine intestinal mucosaNetrin receptor unc-40 experimental SAS data
Netrin unc-6 Isoform a of Netrin receptor unc-5 Heparin, porcine intestinal mucosa Netrin receptor unc-40 Kratky plot
Sample: Netrin unc-6 monomer, 52 kDa Caenorhabditis elegans protein
Isoform a of Netrin receptor unc-5 monomer, 38 kDa Caenorhabditis elegans protein
Heparin, porcine intestinal mucosa monomer, 15 kDa Sus scrofa domesticus
Netrin receptor unc-40 monomer, 118 kDa Caenorhabditis elegans protein
Buffer: 10 mM HEPES pH 7.2, 150 mM NaCl, 100 mM MgSO4, pH: 7.2
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Dec 11
Structural insights into the formation of repulsive netrin guidance complexes. Sci Adv 10(7):eadj8083 (2024)
Priest JM, Nichols EL, Smock RG, Hopkins JB, Mendoza JL, Meijers R, Shen K, Özkan E
RgGuinier 10.6 nm
Dmax 36.5 nm
VolumePorod 3410 nm3

SASDT76 – Neurofilament low intrinsically disordered tail domain aggregate - 2.68 mg/ml - 20 mM Tris pH 8.0

Neurofilament light polypeptide (T445N; C-terminus, amino acids 399-553) experimental SAS data
Neurofilament light polypeptide (T445N; C-terminus, amino acids 399-553) Kratky plot
Sample: Neurofilament light polypeptide (T445N; C-terminus, amino acids 399-553), 16 kDa Mus musculus protein
Buffer: 20 mM Tris, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Sep 7
From isolated polyelectrolytes to star-like assemblies: the role of sequence heterogeneity on the statistical structure of the intrinsically disordered neurofilament-low tail domain. Eur Phys J E Soft Matter 47(2):13 (2024)
Kravikass M, Koren G, Saleh OA, Beck R
RgGuinier 26.4 nm

SASDT86 – Neurofilament low intrinsically disordered tail domain aggregate - 1.80 mg/ml - 20 mM Tris pH 8.0

Neurofilament light polypeptide (T445N; C-terminus, amino acids 399-553) experimental SAS data
Neurofilament light polypeptide (T445N; C-terminus, amino acids 399-553) Kratky plot
Sample: Neurofilament light polypeptide (T445N; C-terminus, amino acids 399-553), 16 kDa Mus musculus protein
Buffer: 20 mM Tris, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Sep 7
From isolated polyelectrolytes to star-like assemblies: the role of sequence heterogeneity on the statistical structure of the intrinsically disordered neurofilament-low tail domain. Eur Phys J E Soft Matter 47(2):13 (2024)
Kravikass M, Koren G, Saleh OA, Beck R
RgGuinier 21.9 nm