Browse by MACROMOLECULE type: protein

SASDRN5 – Nucleolar RNA Chaperone-Like Protein 1 (NURC1)

AT5g04600/T32M21_200 experimental SAS data
GASBOR model
Sample: AT5g04600/T32M21_200 monomer, 25 kDa Arabidopsis thaliana protein
Buffer: 50 mM HNa2PO4, 300 mM NaCl, 5% glycerol (v/v), 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 22
Structural and functional analysis of a plant nucleolar RNA chaperone-like protein. Sci Rep 13(1):9656 (2023)
Fernandes R, Ostendorp A, Ostendorp S, Mehrmann J, Falke S, Graewert MA, Weingartner M, Kehr J, Hoth S
RgGuinier 3.5 nm
Dmax 12.4 nm
VolumePorod 66 nm3

SASDNJ5 – Full length 3-phosphoinositide-dependent protein kinase (PDK1)

3-phosphoinositide-dependent protein kinase 1 experimental SAS data
DAMMIF model
Sample: 3-phosphoinositide-dependent protein kinase 1 monomer, 65 kDa Homo sapiens protein
Buffer: 20 mM Tris-HCl pH 7.4, 250 mM NaCl, 1 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Mar 26
Modulation of the substrate specificity of the kinase PDK1 by distinct conformations of the full-length protein Science Signaling 16(789) (2023)
Sacerdoti M, Gross L, Riley A, Zehnder K, Ghode A, Klinke S, Anand G, Paris K, Winkel A, Herbrand A, Godage H, Cozier G, Süß E, Schulze J, Pastor-Flores D, Bollini M, Cappellari M, Svergun D, Gräwert M, Aramendia P, Leroux A, Potter B, Camacho C, Biondi R
RgGuinier 3.5 nm
Dmax 11.2 nm
VolumePorod 107 nm3

SASDNK5 – Full length 3-phosphoinositide-dependent protein kinase (PDK1) in the presence of HYG8 (2-O-benzoyl-Ins(1,3,4,5,6)P5)

3-phosphoinositide-dependent protein kinase 12-O-benzoyl-Ins(1,3,4,5,6)P5 experimental SAS data
DAMMIF model
Sample: 3-phosphoinositide-dependent protein kinase 1 monomer, 65 kDa Homo sapiens protein
2-O-benzoyl-Ins(1,3,4,5,6)P5 monomer, 1 kDa synthetic construct
Buffer: 20 mM Tris-HCl pH 7.4, 250 mM NaCl, 1 mM DTT, 1 μM HYG8, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Mar 26
Modulation of the substrate specificity of the kinase PDK1 by distinct conformations of the full-length protein Science Signaling 16(789) (2023)
Sacerdoti M, Gross L, Riley A, Zehnder K, Ghode A, Klinke S, Anand G, Paris K, Winkel A, Herbrand A, Godage H, Cozier G, Süß E, Schulze J, Pastor-Flores D, Bollini M, Cappellari M, Svergun D, Gräwert M, Aramendia P, Leroux A, Potter B, Camacho C, Biondi R
RgGuinier 3.5 nm
Dmax 11.5 nm
VolumePorod 98 nm3

SASDNL5 – Double mutant catalytic domain of 3-phosphoinositide-dependent protein kinase 1 (PDK1 50–359; Y188G Q292A)

3-phosphoinositide-dependent protein kinase 1 (Y188G Q292A) experimental SAS data
SREFLEX model
Sample: 3-phosphoinositide-dependent protein kinase 1 (Y188G Q292A) monomer, 35 kDa Homo sapiens protein
Buffer: 20 mM Tris-HCl pH 7.4, 250 mM NaCl, 1 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Mar 26
Modulation of the substrate specificity of the kinase PDK1 by distinct conformations of the full-length protein Science Signaling 16(789) (2023)
Sacerdoti M, Gross L, Riley A, Zehnder K, Ghode A, Klinke S, Anand G, Paris K, Winkel A, Herbrand A, Godage H, Cozier G, Süß E, Schulze J, Pastor-Flores D, Bollini M, Cappellari M, Svergun D, Gräwert M, Aramendia P, Leroux A, Potter B, Camacho C, Biondi R
RgGuinier 2.4 nm
Dmax 7.0 nm
VolumePorod 57 nm3

SASDV53 – Fc-fused PTPRA ECD in PBS buffer

Receptor-type tyrosine-protein phosphatase alpha experimental SAS data
DAMMIF model
Sample: Receptor-type tyrosine-protein phosphatase alpha dimer, 150 kDa Homo sapiens protein
Buffer: 10 mM phosphate, 137 mM NaCl, 2.7 mM KCl, pH: 7.4
Experiment: SAXS data collected at 13A, Taiwan Photon Source, NSRRC on 2021 Aug 19
High Density of N- and O-Glycosylation Shields and Defines the Structural Dynamics of the Intrinsically Disordered Ectodomain of Receptor-type Protein Tyrosine Phosphatase Alpha JACS Au (2023)
Chien Y, Wang Y, Sridharan D, Kuo C, Chien C, Uchihashi T, Kato K, Angata T, Meng T, Hsu S, Khoo K
RgGuinier 8.3 nm
Dmax 35.3 nm

SASDPK6 – N-terminal RNA-binding domain (NTD) of nucleocapsid protein (N) of SARS-CoV-2 in phosphate conditions

Nucleoprotein experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Nucleoprotein monomer, 15 kDa Severe acute respiratory … protein
Buffer: 25 mM potassium phosphate, 150 mM KCl, 2 mM TCEP, pH: 6.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Aug 16
The preference signature of the SARS-CoV-2 Nucleocapsid NTD for its 5'-genomic RNA elements. Nat Commun 14(1):3331 (2023)
Korn SM, Dhamotharan K, Jeffries CM, Schlundt A
RgGuinier 1.6 nm
Dmax 6.1 nm
VolumePorod 23 nm3

SASDR33 – N-terminal RNA-binding domain (NTD) of nucleocapsid protein (N) of SARS-CoV-2 in HEPES conditions

Nucleoprotein experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Nucleoprotein monomer, 15 kDa Severe acute respiratory … protein
Buffer: 25 mM HEPES, 75 mM KCl, 2.5 mM NaNO3, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Nov 29
The preference signature of the SARS-CoV-2 Nucleocapsid NTD for its 5'-genomic RNA elements. Nat Commun 14(1):3331 (2023)
Korn SM, Dhamotharan K, Jeffries CM, Schlundt A
RgGuinier 1.6 nm
Dmax 5.2 nm
VolumePorod 26 nm3

SASDP69 – Acinetobacter baumannii carbonic anhydrase PaaY trimer

Bacterial transferase hexapeptide repeat protein experimental SAS data
CORAL model
Sample: Bacterial transferase hexapeptide repeat protein trimer, 66 kDa Acinetobacter baumannii (strain … protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2021 Jan 29
Mechanistic and structural insights into the bifunctional enzyme PaaY from Acinetobacter baumannii. Structure (2023)
Jiao M, He W, Ouyang Z, Qin Q, Guo Y, Zhang J, Bai Y, Guo X, Yu Q, She J, Hwang PM, Zheng F, Wen Y
RgGuinier 2.6 nm
Dmax 9.0 nm
VolumePorod 102 nm3

SASDSP2 – Saccharomyces cerevisiae Mer2 coiled-coiled with N-terminal SUMO-tag

Recombination protein 107 experimental SAS data
Sample: Recombination protein 107 tetramer, 139 kDa Saccharomyces cerevisiae (strain … protein
Buffer: 25 mM HEPES-NaOH, 500 mM NaCl, 5 mM EDTA, 5% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2023 Apr 14
Evolutionary conservation of the structure and function of meiotic Rec114-Mei4 and Mer2 complexes. Genes Dev 37(11-12):535-553 (2023)
Daccache D, De Jonge E, Liloku P, Mechleb K, Haddad M, Corthaut S, Sterckx YG, Volkov AN, Claeys Bouuaert C
RgGuinier 8.3 nm
Dmax 32.2 nm

SASDSQ2 – Sordaria macrospora ASY2 coiled-coiled with N-terminal SUMO-tag

WGS project CABT00000000 data, contig 2.12 experimental SAS data
OTHER model
Sample: WGS project CABT00000000 data, contig 2.12 tetramer, 155 kDa Sordaria macrospora (strain … protein
Buffer: 25 mM HEPES-NaOH, 500 mM NaCl, 5 mM EDTA, 5% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2023 Apr 14
Evolutionary conservation of the structure and function of meiotic Rec114-Mei4 and Mer2 complexes. Genes Dev 37(11-12):535-553 (2023)
Daccache D, De Jonge E, Liloku P, Mechleb K, Haddad M, Corthaut S, Sterckx YG, Volkov AN, Claeys Bouuaert C
RgGuinier 9.6 nm
Dmax 38.0 nm