Small Angle Scattering Biological Data Bank SASBDB

Browse by MACROMOLECULE type: protein

SASDPK6 – N-terminal RNA-binding domain (NTD) of nucleocapsid protein (N) of SARS-CoV-2 in phosphate conditions

Sample:	Nucleoprotein monomer, 15 kDa Severe acute respiratory … protein
Buffer:	25 mM potassium phosphate, 150 mM KCl, 2 mM TCEP, pH: 6.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Aug 16

The preference signature of the SARS-CoV-2 Nucleocapsid NTD for its 5'-genomic RNA elements. Nat Commun 14(1):3331 (2023)
Korn SM, Dhamotharan K, Jeffries CM, Schlundt A

R_g^Guinier	1.6	nm
D_max	6.1	nm
Volume^Porod	23	nm³

SASDR33 – N-terminal RNA-binding domain (NTD) of nucleocapsid protein (N) of SARS-CoV-2 in HEPES conditions

Sample:	Nucleoprotein monomer, 15 kDa Severe acute respiratory … protein
Buffer:	25 mM HEPES, 75 mM KCl, 2.5 mM NaNO3, pH: 7.2
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2022 Nov 29

The preference signature of the SARS-CoV-2 Nucleocapsid NTD for its 5'-genomic RNA elements. Nat Commun 14(1):3331 (2023)
Korn SM, Dhamotharan K, Jeffries CM, Schlundt A

R_g^Guinier	1.6	nm
D_max	5.2	nm
Volume^Porod	26	nm³

SASDP69 – Acinetobacter baumannii carbonic anhydrase PaaY trimer

Bacterial transferase hexapeptide repeat protein experimental SAS data

Sample:	Bacterial transferase hexapeptide repeat protein trimer, 66 kDa Acinetobacter baumannii (strain … protein
Buffer:	20 mM Tris, 150 mM NaCl, pH: 7.4
Experiment:	SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2021 Jan 29

Mechanistic and structural insights into the bifunctional enzyme PaaY from Acinetobacter baumannii. Structure (2023)
Jiao M, He W, Ouyang Z, Qin Q, Guo Y, Zhang J, Bai Y, Guo X, Yu Q, She J, Hwang PM, Zheng F, Wen Y

R_g^Guinier	2.6	nm
D_max	9.0	nm
Volume^Porod	102	nm³

SASDSP2 – Saccharomyces cerevisiae Mer2 coiled-coiled with N-terminal SUMO-tag

Recombination protein 107 experimental SAS data

Sample:	Recombination protein 107 tetramer, 139 kDa Saccharomyces cerevisiae (strain … protein
Buffer:	25 mM HEPES-NaOH, 500 mM NaCl, 5 mM EDTA, 5% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2023 Apr 14

Evolutionary conservation of the structure and function of meiotic Rec114-Mei4 and Mer2 complexes. Genes Dev 37(11-12):535-553 (2023)
Daccache D, De Jonge E, Liloku P, Mechleb K, Haddad M, Corthaut S, Sterckx YG, Volkov AN, Claeys Bouuaert C

R_g^Guinier	8.3	nm
D_max	32.2	nm

SASDSQ2 – Sordaria macrospora ASY2 coiled-coiled with N-terminal SUMO-tag

WGS project CABT00000000 data, contig 2.12 experimental SAS data

Sample:	WGS project CABT00000000 data, contig 2.12 tetramer, 155 kDa Sordaria macrospora (strain … protein
Buffer:	25 mM HEPES-NaOH, 500 mM NaCl, 5 mM EDTA, 5% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2023 Apr 14

R_g^Guinier	9.6	nm
D_max	38.0	nm

SASDSR2 – Zea mays PAIR1 coiled-coiled with N-terminal SUMO-tag

Sample:	Protein PAIR1 tetramer, 132 kDa Zea mays protein
Buffer:	25 mM HEPES-NaOH, 500 mM NaCl, 5 mM EDTA, 5% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2023 Apr 14

R_g^Guinier	8.0	nm
D_max	31.7	nm

SASDSS2 – Arabidopsis thaliana PRD3 coiled-coiled with N-terminal SUMO-tag

Putative recombination initiation defects 3 experimental SAS data

Sample:	Putative recombination initiation defects 3 tetramer, 129 kDa Arabidopsis thaliana protein
Buffer:	25 mM HEPES-NaOH, 500 mM NaCl, 5 mM EDTA, 5% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2023 Apr 14

R_g^Guinier	7.8	nm
D_max	27.5	nm

SASDST2 – Saccharomyces pombe Rec15 coiled-coiled with N-terminal SUMO-tag

Meiotic recombination protein rec15 experimental SAS data

Sample:	Meiotic recombination protein rec15 tetramer, 128 kDa Schizosaccharomyces pombe (strain … protein
Buffer:	25 mM HEPES-NaOH, 500 mM NaCl, 5 mM EDTA, 5% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2023 Apr 14

R_g^Guinier	6.9	nm
D_max	26.5	nm

SASDSU2 – Mus musculus Iho1 coiled-coiled with N-terminal SUMO-tag

Interactor of HORMAD1 protein 1 (M198V, V230I) experimental SAS data

Sample:	Interactor of HORMAD1 protein 1 (M198V, V230I) tetramer, 127 kDa Mus musculus protein
Buffer:	25 mM HEPES-NaOH, 500 mM NaCl, 5 mM EDTA, 5% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2023 Apr 14

R_g^Guinier	7.2	nm
D_max	25.5	nm

SASDNM8 – Quorum sensing master protein (QseM)

DUF2285 domain-containing protein experimental SAS data

DUF2285 domain-containing protein Kratky plot

Sample:	DUF2285 domain-containing protein monomer, 11 kDa Mesorhizobium loti protein
Buffer:	100 mM NaCl, 50 mM Tris-HCl, 2% glycerol, pH: 7.5
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Dec 1

DUF2285 is a novel helix-turn-helix domain variant that orchestrates both activation and antiactivation of conjugative element transfer in proteobacteria. Nucleic Acids Res (2023)
Jowsey WJ, Morris CRP, Hall DA, Sullivan JT, Fagerlund RD, Eto KY, Solomon PD, Mackay JP, Bond CS, Ramsay JP, Ronson CW

R_g^Guinier	1.5	nm
D_max	5.7	nm
Volume^Porod	15	nm³

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