Browse by MACROMOLECULE type: protein

SASDRR4 – Diffusive GAC rRNA + Mg: Equilibrium final

50S ribosomal protein L11 experimental SAS data
50S ribosomal protein L11 Kratky plot
Sample: 50S ribosomal protein L11 monomer, 16 kDa Thermus thermophilus protein
Buffer: 10 mM sodium cacodylate, 100 mM KCl, pH: 6.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Dec 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
Zielinski K, Katz A, Calvey G, Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 2.2 nm
Dmax 11.0 nm
VolumePorod 27 nm3

SASDRG8 – Astaxanthin-binding protein orange1 (AstaPo1) complexed with zeaxanthin

Astaxanthin binding fasciclin family protein experimental SAS data
PYMOL model
Sample: Astaxanthin binding fasciclin family protein monomer, 22 kDa Coelastrella astaxanthina protein
Buffer: 20 mM Tris-HCl, 150 mM NaCl, pH: 7.6
Experiment: SAXS data collected at BM29, ESRF on 2021 Sep 24
Structural basis for the ligand promiscuity of the neofunctionalized, carotenoid-binding fasciclin domain protein AstaP. Commun Biol 6(1):471 (2023)
Kornilov FD, Slonimskiy YB, Lunegova DA, Egorkin NA, Savitskaya AG, Kleymenov SY, Maksimov EG, Goncharuk SA, Mineev KS, Sluchanko NN
RgGuinier 2.1 nm
Dmax 8.0 nm
VolumePorod 43 nm3

SASDRH8 – Astaxanthin-binding protein orange1 (AstaPo1) complexed with canthaxanthin

Astaxanthin binding fasciclin family protein experimental SAS data
PYMOL model
Sample: Astaxanthin binding fasciclin family protein monomer, 22 kDa Coelastrella astaxanthina protein
Buffer: 20 mM Tris-HCl, 150 mM NaCl, pH: 7.6
Experiment: SAXS data collected at BM29, ESRF on 2021 Sep 24
Structural basis for the ligand promiscuity of the neofunctionalized, carotenoid-binding fasciclin domain protein AstaP. Commun Biol 6(1):471 (2023)
Kornilov FD, Slonimskiy YB, Lunegova DA, Egorkin NA, Savitskaya AG, Kleymenov SY, Maksimov EG, Goncharuk SA, Mineev KS, Sluchanko NN
RgGuinier 2.1 nm
Dmax 8.5 nm
VolumePorod 46 nm3

SASDP99 – invariant surface glycoprotein ISG65

65 kDa invariant surface glycoprotein, putative experimental SAS data
invariant surface glycoprotein ISG65 Rg histogram
Sample: 65 kDa invariant surface glycoprotein, putative monomer, 41 kDa Trypanosoma brucei gambiense … protein
Buffer: 20 mM HEPES, 150 mM NaCl, 3% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2020 Nov 25
Cryo-EM structures of Trypanosoma brucei gambiense ISG65 with human complement C3 and C3b and their roles in alternative pathway restriction. Nat Commun 14(1):2403 (2023)
Sülzen H, Began J, Dhillon A, Kereïche S, Pompach P, Votrubova J, Zahedifard F, Šubrtova A, Šafner M, Hubalek M, Thompson M, Zoltner M, Zoll S
RgGuinier 3.3 nm
Dmax 12.5 nm
VolumePorod 53 nm3

SASDPA9 – invariant surface glycoprotein ISG65 bound to complement C3 (C3d)

65 kDa invariant surface glycoprotein, putativeComplement C3 experimental SAS data
DAMMIF model
Sample: 65 kDa invariant surface glycoprotein, putative monomer, 41 kDa Trypanosoma brucei gambiense … protein
Complement C3 monomer, 35 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 3% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2020 Nov 25
Cryo-EM structures of Trypanosoma brucei gambiense ISG65 with human complement C3 and C3b and their roles in alternative pathway restriction. Nat Commun 14(1):2403 (2023)
Sülzen H, Began J, Dhillon A, Kereïche S, Pompach P, Votrubova J, Zahedifard F, Šubrtova A, Šafner M, Hubalek M, Thompson M, Zoltner M, Zoll S
RgGuinier 3.5 nm
Dmax 12.8 nm
VolumePorod 104 nm3

SASDRL8 – Bovine serum albumin

Albumin experimental SAS data
Albumin Kratky plot
Sample: Albumin monomer, 66 kDa Bos taurus protein
Buffer: 20 mM HEPES, 150 mM NaCl, pH: 7.2
Experiment: SAXS data collected at 13A, Taiwan Photon Source, NSRRC on 2022 Jun 7
NSRRC TPS13A standard protein archive
Orion Shih
RgGuinier 2.9 nm
Dmax 8.9 nm
VolumePorod 84 nm3

SASDRM8 – Lysozyme C (Gallus gallus)

Lysozyme C experimental SAS data
Lysozyme C Kratky plot
Sample: Lysozyme C monomer, 14 kDa Gallus gallus protein
Buffer: 40 mM sodium acetate, 50 mM NaCl, pH: 4
Experiment: SAXS data collected at 13A, Taiwan Photon Source, NSRRC on 2021 Apr 2
NSRRC TPS13A standard protein archive
Orion Shih
RgGuinier 1.4 nm
Dmax 4.6 nm
VolumePorod 13 nm3

SASDRN8 – α/β Hemoglobin (human)

Hemoglobin subunit alphaHemoglobin subunit beta experimental SAS data
Hemoglobin subunit alpha Hemoglobin subunit beta Kratky plot
Sample: Hemoglobin subunit alpha dimer, 31 kDa Homo sapiens protein
Hemoglobin subunit beta dimer, 32 kDa Homo sapiens protein
Buffer: phosphate buffered saline, pH: 7.5
Experiment: SAXS data collected at 13A, Taiwan Photon Source, NSRRC on 2021 Oct 7
NSRRC TPS13A standard protein archive
Orion Shih
RgGuinier 2.5 nm
Dmax 9.2 nm
VolumePorod 97 nm3

SASDPL4 – Human MUC2 mucin C-terminal dimer

Human Mucin 2 C-terminal experimental SAS data
DAMMIN model
Sample: Human Mucin 2 C-terminal dimer, 240 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 100 mM NaCl, 10 mM CaCl2, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2020 Nov 20
The intestinal MUC2 mucin C-terminus is stabilized by an extra disulfide bond in comparison to von Willebrand factor and other gel-forming mucins Nature Communications 14(1) (2023)
Gallego P, Garcia-Bonete M, Trillo-Muyo S, Recktenwald C, Johansson M, Hansson G
RgGuinier 8.4 nm
Dmax 32.0 nm
VolumePorod 684 nm3

SASDRU7 – Angiotensin-converting enzyme 2/immunoglobulin-Fc chimera protein (Ace2-Fc) at 3.81 mg/ml

Angiotensin-converting enzyme 2 chimera with the Fc region of the immunoglobulin heavy constant gamma 4 experimental SAS data
Angiotensin-converting enzyme 2 chimera with the Fc region of the immunoglobulin heavy constant gamma 4 Kratky plot
Sample: Angiotensin-converting enzyme 2 chimera with the Fc region of the immunoglobulin heavy constant gamma 4 dimer, 217 kDa Homo sapiens protein
Buffer: 50 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at Rigaku BioSAXS-1000, SFB 1035, Technische Universität München on 2021 Jul 5
Extrinsic stabilization of antiviral ACE2-Fc fusion proteins targeting SARS-CoV-2. Commun Biol 6(1):386 (2023)
Svilenov HL, Delhommel F, Siebenmorgen T, Rührnößl F, Popowicz GM, Reiter A, Sattler M, Brockmeyer C, Buchner J
RgGuinier 5.5 nm
Dmax 18.6 nm
VolumePorod 408 nm3