Small Angle Scattering Biological Data Bank SASBDB

Browse by MACROMOLECULE type: protein

SASDRV3 – Trypsin + Aprotinin: Time-Resolved 1000 ms

Serine protease 1Pancreatic trypsin inhibitor experimental SAS data

Serine protease 1 Pancreatic trypsin inhibitor Kratky plot

Sample:	Serine protease 1 monomer, 26 kDa Bos taurus protein Pancreatic trypsin inhibitor monomer, 11 kDa Bos taurus protein
Buffer:	20 mM TRIS, 40 mM KCl, 20 mM CaCl2, pH: 7
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9

Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
Zielinski K, Katz A, Calvey G, Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L

R_g^Guinier	1.8	nm
D_max	5.3	nm
Volume^Porod	28	nm³

SASDRW3 – Trypsin + Aprotinin: Time-Resolved 2000 ms

Sample:	Serine protease 1 monomer, 26 kDa Bos taurus protein Pancreatic trypsin inhibitor monomer, 11 kDa Bos taurus protein
Buffer:	20 mM TRIS, 40 mM KCl, 20 mM CaCl2, pH: 7
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9

R_g^Guinier	2.0	nm
D_max	5.3	nm
Volume^Porod	29	nm³

SASDRX3 – Trypsin + Aprotinin: Complex equilibrium

Sample:	Serine protease 1 monomer, 26 kDa Bos taurus protein Pancreatic trypsin inhibitor monomer, 11 kDa Bos taurus protein
Buffer:	20 mM TRIS, 40 mM KCl, 20 mM CaCl2, pH: 7
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9

R_g^Guinier	1.9	nm
D_max	5.3	nm
Volume^Porod	31	nm³

SASDRR4 – Diffusive GAC rRNA + Mg: Equilibrium final

50S ribosomal protein L11 experimental SAS data

Sample:	50S ribosomal protein L11 monomer, 16 kDa Thermus thermophilus protein
Buffer:	10 mM sodium cacodylate, 100 mM KCl, pH: 6.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Dec 9

R_g^Guinier	2.2	nm
D_max	11.0	nm
Volume^Porod	27	nm³

SASDRG8 – Astaxanthin-binding protein orange1 (AstaPo1) complexed with zeaxanthin

Astaxanthin binding fasciclin family protein experimental SAS data

Sample:	Astaxanthin binding fasciclin family protein monomer, 22 kDa Coelastrella astaxanthina protein
Buffer:	20 mM Tris-HCl, 150 mM NaCl, pH: 7.6
Experiment:	SAXS data collected at BM29, ESRF on 2021 Sep 24

Structural basis for the ligand promiscuity of the neofunctionalized, carotenoid-binding fasciclin domain protein AstaP. Commun Biol 6(1):471 (2023)
Kornilov FD, Slonimskiy YB, Lunegova DA, Egorkin NA, Savitskaya AG, Kleymenov SY, Maksimov EG, Goncharuk SA, Mineev KS, Sluchanko NN

R_g^Guinier	2.1	nm
D_max	8.0	nm
Volume^Porod	43	nm³

SASDRH8 – Astaxanthin-binding protein orange1 (AstaPo1) complexed with canthaxanthin

Sample:	Astaxanthin binding fasciclin family protein monomer, 22 kDa Coelastrella astaxanthina protein
Buffer:	20 mM Tris-HCl, 150 mM NaCl, pH: 7.6
Experiment:	SAXS data collected at BM29, ESRF on 2021 Sep 24

R_g^Guinier	2.1	nm
D_max	8.5	nm
Volume^Porod	46	nm³

SASDP99 – invariant surface glycoprotein ISG65

65 kDa invariant surface glycoprotein, putative experimental SAS data

invariant surface glycoprotein ISG65 Rg histogram

Sample:	65 kDa invariant surface glycoprotein, putative monomer, 41 kDa Trypanosoma brucei gambiense … protein
Buffer:	20 mM HEPES, 150 mM NaCl, 3% (v/v) glycerol, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2020 Nov 25

Cryo-EM structures of Trypanosoma brucei gambiense ISG65 with human complement C3 and C3b and their roles in alternative pathway restriction. Nat Commun 14(1):2403 (2023)
Sülzen H, Began J, Dhillon A, Kereïche S, Pompach P, Votrubova J, Zahedifard F, Šubrtova A, Šafner M, Hubalek M, Thompson M, Zoltner M, Zoll S

R_g^Guinier	3.3	nm
D_max	12.5	nm
Volume^Porod	53	nm³

SASDPA9 – invariant surface glycoprotein ISG65 bound to complement C3 (C3d)

65 kDa invariant surface glycoprotein, putativeComplement C3 experimental SAS data

Sample:	65 kDa invariant surface glycoprotein, putative monomer, 41 kDa Trypanosoma brucei gambiense … protein Complement C3 monomer, 35 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, 3% (v/v) glycerol, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2020 Nov 25

R_g^Guinier	3.5	nm
D_max	12.8	nm
Volume^Porod	104	nm³

SASDRL8 – Bovine serum albumin

Sample:	Albumin monomer, 66 kDa Bos taurus protein
Buffer:	20 mM HEPES, 150 mM NaCl, pH: 7.2
Experiment:	SAXS data collected at 13A, Taiwan Photon Source, NSRRC on 2022 Jun 7

NSRRC TPS13A standard protein archive
Orion Shih

R_g^Guinier	2.9	nm
D_max	8.9	nm
Volume^Porod	84	nm³

SASDRM8 – Lysozyme C (Gallus gallus)

Sample:	Lysozyme C monomer, 14 kDa Gallus gallus protein
Buffer:	40 mM sodium acetate, 50 mM NaCl, pH: 4
Experiment:	SAXS data collected at 13A, Taiwan Photon Source, NSRRC on 2021 Apr 2

NSRRC TPS13A standard protein archive
Orion Shih

R_g^Guinier	1.4	nm
D_max	4.6	nm
Volume^Porod	13	nm³

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