Browse by MACROMOLECULE type: protein

SASDJH5 – SARS-CoV-2 Main Protease, P9T mutant

3C-like proteinase from SARS-CoV-2 replicase polyprotein 1a, PT9 mutant experimental SAS data
SREFLEX model
Sample: 3C-like proteinase from SARS-CoV-2 replicase polyprotein 1a, PT9 mutant monomer, 34 kDa Severe acute respiratory … protein
Buffer: 50 mM Tris, 1 mM DTT, 1 mM EDTA, pH: 7.4
Experiment: SAXS data collected at Rigaku BioSAXS-2000, University of British Columbia on 2020 Jun 1
Crystallographic structure of wild-type SARS-CoV-2 main protease acyl-enzyme intermediate with physiological C-terminal autoprocessing site. Nat Commun 11(1):5877 (2020)
Lee J, Worrall LJ, Vuckovic M, Rosell FI, Gentile F, Ton AT, Caveney NA, Ban F, Cherkasov A, Paetzel M, Strynadka NCJ
RgGuinier 2.4 nm
Dmax 7.2 nm
VolumePorod 54 nm3

SASDHG6 – N-terminal engineered, disulfide-containing endo-beta-N-acetylglucosaminidase H at 36.3 Gy X-ray dose (J/kg)

Endo-beta-N-acetylglucosaminidase H experimental SAS data
Endo-beta-N-acetylglucosaminidase H Kratky plot
Sample: Endo-beta-N-acetylglucosaminidase H dimer, 61 kDa Streptomyces plicatus protein
Buffer: 20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Nov 4
SAXS studies of X-ray induced disulfide bond damage: Engineering high-resolution insight from a low-resolution technique PLOS ONE 15(11):e0239702 (2020)
Stachowski T, Snell M, Snell E, Boggon T
RgGuinier 3.0 nm
VolumePorod 69 nm3

SASDHH6 – N-terminal engineered, disulfide-containing endo-beta-N-acetylglucosaminidase H at 72.6 Gy X-ray dose (J/kg)

Endo-beta-N-acetylglucosaminidase H experimental SAS data
Endo-beta-N-acetylglucosaminidase H Kratky plot
Sample: Endo-beta-N-acetylglucosaminidase H dimer, 61 kDa Streptomyces plicatus protein
Buffer: 20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Nov 4
SAXS studies of X-ray induced disulfide bond damage: Engineering high-resolution insight from a low-resolution technique PLOS ONE 15(11):e0239702 (2020)
Stachowski T, Snell M, Snell E, Boggon T
RgGuinier 3.1 nm
VolumePorod 68 nm3

SASDHJ6 – N-terminal engineered, disulfide-containing endo-beta-N-acetylglucosaminidase H at 108.9 Gy X-ray dose (J/kg)

Endo-beta-N-acetylglucosaminidase H experimental SAS data
Endo-beta-N-acetylglucosaminidase H Kratky plot
Sample: Endo-beta-N-acetylglucosaminidase H dimer, 61 kDa Streptomyces plicatus protein
Buffer: 20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Nov 4
SAXS studies of X-ray induced disulfide bond damage: Engineering high-resolution insight from a low-resolution technique PLOS ONE 15(11):e0239702 (2020)
Stachowski T, Snell M, Snell E, Boggon T
RgGuinier 3.0 nm
VolumePorod 67 nm3

SASDHK6 – N-terminal engineered, disulfide-containing endo-beta-N-acetylglucosaminidase H at 145.2 Gy X-ray dose (J/kg)

Endo-beta-N-acetylglucosaminidase H experimental SAS data
Endo-beta-N-acetylglucosaminidase H Kratky plot
Sample: Endo-beta-N-acetylglucosaminidase H dimer, 61 kDa Streptomyces plicatus protein
Buffer: 20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Nov 4
SAXS studies of X-ray induced disulfide bond damage: Engineering high-resolution insight from a low-resolution technique PLOS ONE 15(11):e0239702 (2020)
Stachowski T, Snell M, Snell E, Boggon T
RgGuinier 3.0 nm
VolumePorod 66 nm3

SASDHL6 – N-terminal engineered, disulfide-containing endo-beta-N-acetylglucosaminidase H at 181.5 Gy X-ray dose (J/kg)

Endo-beta-N-acetylglucosaminidase H experimental SAS data
Endo-beta-N-acetylglucosaminidase H Kratky plot
Sample: Endo-beta-N-acetylglucosaminidase H dimer, 61 kDa Streptomyces plicatus protein
Buffer: 20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Nov 4
SAXS studies of X-ray induced disulfide bond damage: Engineering high-resolution insight from a low-resolution technique PLOS ONE 15(11):e0239702 (2020)
Stachowski T, Snell M, Snell E, Boggon T
RgGuinier 2.9 nm
VolumePorod 65 nm3

SASDHM6 – N-terminal engineered, disulfide-containing endo-beta-N-acetylglucosaminidase H at 217.8 Gy X-ray dose (J/kg)

Endo-beta-N-acetylglucosaminidase H experimental SAS data
Endo-beta-N-acetylglucosaminidase H Kratky plot
Sample: Endo-beta-N-acetylglucosaminidase H dimer, 61 kDa Streptomyces plicatus protein
Buffer: 20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Nov 4
SAXS studies of X-ray induced disulfide bond damage: Engineering high-resolution insight from a low-resolution technique PLOS ONE 15(11):e0239702 (2020)
Stachowski T, Snell M, Snell E, Boggon T
RgGuinier 2.9 nm
VolumePorod 64 nm3

SASDHN6 – N-terminal engineered, disulfide-containing endo-beta-N-acetylglucosaminidase H at 254.1 Gy X-ray dose (J/kg)

Endo-beta-N-acetylglucosaminidase H experimental SAS data
Endo-beta-N-acetylglucosaminidase H Kratky plot
Sample: Endo-beta-N-acetylglucosaminidase H dimer, 61 kDa Streptomyces plicatus protein
Buffer: 20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Nov 4
SAXS studies of X-ray induced disulfide bond damage: Engineering high-resolution insight from a low-resolution technique PLOS ONE 15(11):e0239702 (2020)
Stachowski T, Snell M, Snell E, Boggon T
RgGuinier 2.9 nm
VolumePorod 64 nm3

SASDHP6 – N-terminal engineered, disulfide-containing endo-beta-N-acetylglucosaminidase H at 290.4 Gy X-ray dose (J/kg)

Endo-beta-N-acetylglucosaminidase H experimental SAS data
Endo-beta-N-acetylglucosaminidase H Kratky plot
Sample: Endo-beta-N-acetylglucosaminidase H dimer, 61 kDa Streptomyces plicatus protein
Buffer: 20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Nov 4
SAXS studies of X-ray induced disulfide bond damage: Engineering high-resolution insight from a low-resolution technique PLOS ONE 15(11):e0239702 (2020)
Stachowski T, Snell M, Snell E, Boggon T
RgGuinier 2.9 nm
VolumePorod 63 nm3

SASDHQ6 – N-terminal engineered, disulfide-containing endo-beta-N-acetylglucosaminidase H at 326.7 Gy X-ray dose (J/kg)

Endo-beta-N-acetylglucosaminidase H experimental SAS data
Endo-beta-N-acetylglucosaminidase H Kratky plot
Sample: Endo-beta-N-acetylglucosaminidase H dimer, 61 kDa Streptomyces plicatus protein
Buffer: 20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Nov 4
SAXS studies of X-ray induced disulfide bond damage: Engineering high-resolution insight from a low-resolution technique PLOS ONE 15(11):e0239702 (2020)
Stachowski T, Snell M, Snell E, Boggon T
RgGuinier 2.8 nm
VolumePorod 62 nm3