Small Angle Scattering Biological Data Bank SASBDB

Browse by MACROMOLECULE type: protein

SASDHU5 – Heat shock protein 70 (PfHSP70-1)

Heat shock protein 70Peptide experimental SAS data

Sample:	Heat shock protein 70 monomer, 74 kDa Plasmodium falciparum protein Peptide monomer, 1 kDa synthetic construct protein
Buffer:	50mM Tris, 5mM MgCl2, 500mM KCl, 5mM Bme, 5% glycerol, pH: 8
Experiment:	SAXS data collected at Anton Paar SAXSpace, CSIR-Central Drug Research Institute on 2019 Oct 24

Structural-functional diversity of malaria parasite's PfHSP70-1 and PfHSP40 chaperone pair gives an edge over human orthologs in chaperone-assisted protein folding. Biochem J 477(18):3625-3643 (2020)
Anas M, Shukla A, Tripathi A, Kumari V, Prakash C, Nag P, Kumar LS, Sharma SK, Ramachandran R, Kumar N

R_g^Guinier	5.3	nm
D_max	16.6	nm
Volume^Porod	450	nm³

SASDJU9 – Mammalian cell entry protein 1A (Mce1A36-148)

Mce-family protein Mce1A monomer experimental SAS data

Sample:	Mce-family protein Mce1A monomer monomer, 17 kDa Mycobacterium tuberculosis protein
Buffer:	50 mM Tris, 350 mM NaCl, 10% Glycerol, pH: 8.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 May 10

Structural insights into the substrate-binding proteins Mce1A and Mce4A from Mycobacterium tuberculosis IUCrJ 8(5) (2021)
Asthana P, Singh D, Pedersen J, Hynönen M, Sulu R, Murthy A, Laitaoja M, Jänis J, Riley L, Venkatesan R

R_g^Guinier	2.1	nm
D_max	8.7	nm
Volume^Porod	29	nm³

SASDJV9 – Mammalian cell entry protein 4A (Mce4A39-140)

Mce-family protein Mce4A experimental SAS data

Sample:	Mce-family protein Mce4A monomer, 15 kDa Mycobacterium tuberculosis (strain … protein
Buffer:	50mM MOPS, 350mM NaCl, 10% Glycerol, pH: 7
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 May 10

R_g^Guinier	2.1	nm
D_max	7.8	nm
Volume^Porod	22	nm³

SASDJW9 – Mammalian cell entry protein 4A (Mce4A36-400)

Mce-family protein Mce4An-Dodecyl-β-D-Maltopyranoside experimental SAS data

Sample:	Mce-family protein Mce4A monomer, 44 kDa Mycobacterium tuberculosis (strain … protein N-Dodecyl-β-D-Maltopyranoside 0, 102 kDa
Buffer:	50mM Tris, 500mM NaCl, 10% Glycerol, 5mM DDM, 1mM Beta-ME, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 13

R_g^Guinier	5.7	nm
D_max	21.5	nm
Volume^Porod	446	nm³

SASDJX9 – Mammalian cell entry protein 4A (Mce4A121-400)

n-Dodecyl-β-D-MaltopyranosideMce-family protein Mce4A experimental SAS data

Sample:	N-Dodecyl-β-D-Maltopyranoside 0, 102 kDa Mce-family protein Mce4A monomer, 35 kDa Mycobacterium tuberculosis (strain … protein
Buffer:	50mM Tris, 500mM NaCl, 10% Glycerol, 5mM DDM, 1mM Beta-ME, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 Nov 28

R_g^Guinier	5.0	nm
D_max	19.1	nm
Volume^Porod	278	nm³

SASDJY9 – Mammalian cell entry protein 4A (Mce4A39-320)

Sample:	N-Dodecyl-β-D-Maltopyranoside 0, 102 kDa Mce-family protein Mce4A monomer, 34 kDa Mycobacterium tuberculosis (strain … protein
Buffer:	50mM Tris, 500mM NaCl, 10% Glycerol, 5mM DDM, 1mM Beta-ME, pH: 8.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 May 2

R_g^Guinier	4.8	nm
D_max	18.2	nm
Volume^Porod	189	nm³

SASDJZ9 – Mammalian cell entry protein 1A (Mce1A38-325)

Mce-family protein Mce1An-Dodecyl-β-D-Maltopyranoside experimental SAS data

Sample:	Mce-family protein Mce1A monomer, 36 kDa Mycobacterium tuberculosis protein N-Dodecyl-β-D-Maltopyranoside 0, 82 kDa
Buffer:	50 mM Tris, 500 mM NaCl, 10% Glycerol, 5 mM DDM, 1 mM β-ME, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 May 2

R_g^Guinier	4.6	nm
D_max	17.5	nm
Volume^Porod	151	nm³

SASDK22 – Mammalian cell entry protein 1A (Mce1A126-454)

Sample:	Mce-family protein Mce1A monomer, 39 kDa Mycobacterium tuberculosis protein N-Dodecyl-β-D-Maltopyranoside 0, 123 kDa
Buffer:	50 mM Tris, 350 mM NaCl, 10% Glycerol, 5mM DDM, 1 mM β-ME, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 Nov 28

R_g^Guinier	5.5	nm
D_max	22.0	nm
Volume^Porod	216	nm³

SASDK32 – Mammalian cell entry protein 1A (Mce1A38-454)

Sample:	Mce-family protein Mce1A monomer, 48 kDa Mycobacterium tuberculosis protein N-Dodecyl-β-D-Maltopyranoside 0, 112 kDa
Buffer:	50 mM Tris, 500 mM NaCl, 10% Glycerol, 5mM DDM, 1 mM β-ME, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 13

R_g^Guinier	5.3	nm
D_max	21.6	nm
Volume^Porod	362	nm³

SASDKJ4 – SARS-CoV-2 nsp7 and nsp8

Replicase polyprotein 1a - nsp7Replicase polyprotein 1a - nsp8 experimental SAS data

Sample:	Replicase polyprotein 1a - nsp7, 18 kDa Severe acute respiratory … protein Replicase polyprotein 1a - nsp8, 44 kDa Severe acute respiratory … protein
Buffer:	50 mM Tris, pH 8.0, 100 mM NaCl, 4 mM DTT, 4 mM MgCl2, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Jun 19

Hallmarks of Alpha- and Betacoronavirus non-structural protein 7+8 complexes Science Advances 7(10):eabf1004 (2021)
Krichel B, Bylapudi G, Schmidt C, Blanchet C, Schubert R, Brings L, Koehler M, Zenobi R, Svergun D, Lorenzen K, Madhugiri R, Ziebuhr J, Uetrecht C

R_g^Guinier	3.4	nm
D_max	13.5	nm
Volume^Porod	92	nm³

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