Browse by MACROMOLECULE type: protein

SASDFX2 – Cell wall synthesis protein Wag31(T73A) polymer (SEC-frames 305-309)

Cell wall synthesis protein Wag31 experimental SAS data
Cell wall synthesis protein Wag31 Kratky plot
Sample: Cell wall synthesis protein Wag31, 30 kDa Mycobacterium tuberculosis protein
Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29
Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31. J Struct Biol :107429 (2019)
Choukate K, Gupta A, Basu B, Virk K, Ganguli M, Chaudhuri B
RgGuinier 11.1 nm

SASDFY2 – Cell wall synthesis protein Wag31(T73A) polymer (SEC-frames 310-314)

Cell wall synthesis protein Wag31 experimental SAS data
Cell wall synthesis protein Wag31 Kratky plot
Sample: Cell wall synthesis protein Wag31, 30 kDa Mycobacterium tuberculosis protein
Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29
Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31. J Struct Biol :107429 (2019)
Choukate K, Gupta A, Basu B, Virk K, Ganguli M, Chaudhuri B
RgGuinier 10.6 nm

SASDFZ2 – Cell wall synthesis protein Wag31(T73A) polymer (SEC-frames 315-319)

Cell wall synthesis protein Wag31 experimental SAS data
Cell wall synthesis protein Wag31 Kratky plot
Sample: Cell wall synthesis protein Wag31, 30 kDa Mycobacterium tuberculosis protein
Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29
Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31. J Struct Biol :107429 (2019)
Choukate K, Gupta A, Basu B, Virk K, Ganguli M, Chaudhuri B
RgGuinier 12.1 nm

SASDF23 – Cell wall synthesis protein Wag31(T73A) polymer (SEC-frames 320-324)

Cell wall synthesis protein Wag31 experimental SAS data
Cell wall synthesis protein Wag31 Kratky plot
Sample: Cell wall synthesis protein Wag31, 30 kDa Mycobacterium tuberculosis protein
Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29
Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31. J Struct Biol :107429 (2019)
Choukate K, Gupta A, Basu B, Virk K, Ganguli M, Chaudhuri B
RgGuinier 10.2 nm

SASDKS3 – The dimeric ectodomain of the alkali-sensing insulin receptor–related receptor (ectoIRR) at pH7

Insulin receptor-related protein experimental SAS data
CORAL model
Sample: Insulin receptor-related protein dimer, 201 kDa Homo sapiens protein
Buffer: 150 mM NaCl, 20 mM Tris, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Oct 22
The dimeric ectodomain of the alkali-sensing insulin receptor-related receptor (ectoIRR) has a droplike shape. J Biol Chem 294(47):17790-17798 (2019)
Shtykova EV, Petoukhov MV, Mozhaev AA, Deyev IE, Dadinova LA, Loshkarev NA, Goryashchenko AS, Bocharov EV, Jeffries CM, Svergun DI, Batishchev OV, Petrenko AG
RgGuinier 5.4 nm
Dmax 19.5 nm
VolumePorod 444 nm3

SASDKT3 – The dimeric ectodomain of the alkali-sensing insulin receptor–related receptor (ectoIRR) at pH9

Insulin receptor-related protein experimental SAS data
CORAL model
Sample: Insulin receptor-related protein dimer, 201 kDa Homo sapiens protein
Buffer: 150 mM NaCl, 20 mM Tris, pH: 9
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Oct 22
The dimeric ectodomain of the alkali-sensing insulin receptor-related receptor (ectoIRR) has a droplike shape. J Biol Chem 294(47):17790-17798 (2019)
Shtykova EV, Petoukhov MV, Mozhaev AA, Deyev IE, Dadinova LA, Loshkarev NA, Goryashchenko AS, Bocharov EV, Jeffries CM, Svergun DI, Batishchev OV, Petrenko AG
RgGuinier 5.3 nm
Dmax 19.0 nm
VolumePorod 430 nm3

SASDF96 – Olfactomedin-1 BMY isoform

Noelin experimental SAS data
DAMMIF model
Sample: Noelin tetramer, 72 kDa Mus musculus protein
Buffer: 150 mM NaCl, 20 mM HEPES, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2016 Feb 5
Design and structural characterisation of olfactomedin-1 variants as tools for functional studies. BMC Mol Cell Biol 20(1):50 (2019)
Pronker MF, van den Hoek H, Janssen BJC
RgGuinier 5.4 nm
Dmax 16.3 nm
VolumePorod 160 nm3

SASDE32 – Mothers against decapentaplegic homolog 5 - Smad5 MH1

Mothers against decapentaplegic homolog 5 experimental SAS data
Mothers against decapentaplegic homolog 5 Kratky plot
Sample: Mothers against decapentaplegic homolog 5, 15 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.2
Experiment: SAXS data collected at BM29, ESRF on 2018 Feb 14
Unveiling the dimer/monomer propensities of Smad MH1-DNA complexes (2019)
Ruiz L, Kaczmarska Z, Gomes T, Aragón E, Torner C, Freier R, Bagiński B, Martin-Malpartida P, de Martin Garrido N, Márquez J, Cordeiro T, Pluta R, Macias M
RgGuinier 1.9 nm
Dmax 6.6 nm
VolumePorod 32 nm3

SASDE42 – Mothers against decapentaplegic homolog 8_9 - Smad8_9 MH1

Mothers against decapentaplegic homolog 8_9 experimental SAS data
Mothers against decapentaplegic homolog 8_9 Kratky plot
Sample: Mothers against decapentaplegic homolog 8_9, 15 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.2
Experiment: SAXS data collected at BM29, ESRF on 2018 Mar 1
Unveiling the dimer/monomer propensities of Smad MH1-DNA complexes (2019)
Ruiz L, Kaczmarska Z, Gomes T, Aragón E, Torner C, Freier R, Bagiński B, Martin-Malpartida P, de Martin Garrido N, Márquez J, Cordeiro T, Pluta R, Macias M
RgGuinier 1.9 nm
Dmax 6.5 nm
VolumePorod 34 nm3

SASDFZ3 – Escherichia coli YjhC

Escherichia coli YjhC experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Escherichia coli YjhC dimer, 86 kDa Escherichia coli protein
Buffer: 20 mM Tris, 150 mM NaCl, 0.1 % (w/v) sodium azide, 5 % (v/v) glycerol, pH: 8
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2018 Apr 12
On the structure and function of Escherichia coli YjhC: an oxidoreductase involved in bacterial sialic acid metabolism. Proteins (2019)
Horne CR, Kind L, Davies JS, Dobson RCJ
RgGuinier 3.1 nm
Dmax 10.7 nm
VolumePorod 130 nm3