Small Angle Scattering Biological Data Bank SASBDB

Browse by MACROMOLECULE type: protein

SASDH25 – Actinobacterial phosphodiesterase targeting cyclic di-AMP (AtaC)

Alkaline phosphodiesterase I or Nucleotide pyrophosphatase experimental SAS data

Alkaline phosphodiesterase I or Nucleotide pyrophosphatase Kratky plot

Sample:	Alkaline phosphodiesterase I or Nucleotide pyrophosphatase monomer, 46 kDa Streptomyces venezuelae protein
Buffer:	20 mM HEPES, 200mM NaCl, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2018 May 17

c-di-AMP hydrolysis by a novel type of phosphodiesterase promotes differentiation of multicellular bacteria (2019)
Latoscha A, Drexler D, Al-Bassam M, Kaever V, Findlay K, Witte G, Tschowri N

R_g^Guinier	2.4	nm
D_max	7.8	nm
Volume^Porod	74	nm³

SASDH35 – Soluble cyclic di-AMP binding protein CpeA (vnz_28055), regulator of CpeB

Cyclic di-AMP binding protein (Putative regulatory, ligand-binding protein) experimental SAS data

Cyclic di-AMP binding protein (Putative regulatory, ligand-binding protein) Kratky plot

Sample:	Cyclic di-AMP binding protein (Putative regulatory, ligand-binding protein) dimer, 38 kDa Streptomyces venezuelae protein
Buffer:	200 mM NaCl, 30 mM NaPi, 5% (v/v) glycerol, pH: 6.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Nov 4

c-di-AMP hydrolysis by a novel type of phosphodiesterase promotes differentiation of multicellular bacteria (2019)
Latoscha A, Drexler D, Al-Bassam M, Kaever V, Findlay K, Witte G, Tschowri N

R_g^Guinier	2.8	nm
D_max	9.2	nm
Volume^Porod	69	nm³

SASDHA2 – Soluble guanylate cyclase (unbound)

Soluble guanylyl cyclase alpha-1 subunitSoluble guanylyl cyclase beta-1 subunit experimental SAS data

Sample:	Soluble guanylyl cyclase alpha-1 subunit monomer, 78 kDa Manduca sexta protein Soluble guanylyl cyclase beta-1 subunit monomer, 68 kDa Manduca sexta protein
Buffer:	50 mM KH2PO4, 150 mM NaCl, 2% glycerol,, pH: 7.4
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Dec 3

Allosteric activation of the nitric oxide receptor soluble guanylate cyclase mapped by cryo-electron microscopy. Elife 8 (2019)
Horst BG, Yokom AL, Rosenberg DJ, Morris KL, Hammel M, Hurley JH, Marletta MA

R_g^Guinier	4.3	nm
D_max	13.3	nm
Volume^Porod	230	nm³

SASDHB2 – Soluble guanylate cyclase (nitric oxide, NO, bound)

Sample:	Soluble guanylyl cyclase alpha-1 subunit monomer, 78 kDa Manduca sexta protein Soluble guanylyl cyclase beta-1 subunit monomer, 68 kDa Manduca sexta protein
Buffer:	50 mM KH2PO4,150 mM NaCl, 2% glycerol, 500 µM NO,, pH: 7.4
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Dec 3

R_g^Guinier	4.4	nm
D_max	14.2	nm
Volume^Porod	218	nm³

SASDF58 – Delta subunit of RNA polymerase, RNAP (B. subtilis), 10mM NaCl

DNA-directed RNA polymerase subunit delta experimental SAS data

DNA-directed RNA polymerase subunit delta Kratky plot

Sample:	DNA-directed RNA polymerase subunit delta monomer, 20 kDa Bacillus subtilis protein
Buffer:	20 mM Phosphate buffer, 10 mM NaCl, 0.05% NaN3, pH: 6.6
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Oct 3

Quantitative Conformational Analysis of Functionally Important Electrostatic Interactions in the Intrinsically Disordered Region of Delta Subunit of Bacterial RNA Polymerase. J Am Chem Soc (2019)
Kuban V, Srb P, Stegnerova H, Padrta P, Zachrdla M, Jasenakova Z, Šanderová H, Vítovská D, Krasny L, Koval T, Dohnalek J, Ziemska-Legi Cka J, Grynberg M, Jarnot P, Gruca A, Jensen MR, Blackledge M, Zidek L

R_g^Guinier	3.5	nm
D_max	14.0	nm
Volume^Porod	38	nm³

SASDF68 – Delta subunit of RNA polymerase, RNAP (B. subtilis), 200 mM NaCl

Sample:	DNA-directed RNA polymerase subunit delta monomer, 20 kDa Bacillus subtilis protein
Buffer:	20 mM Phosphate buffer, 200 mM NaCl, 0.05% NaN3, pH: 6.6
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Oct 3

R_g^Guinier	3.9	nm
D_max	20.0	nm
Volume^Porod	56	nm³

SASDF78 – Delta subunit of RNA polymerase, RNAP (B. subtilis), 400 mM NaCl

Sample:	DNA-directed RNA polymerase subunit delta monomer, 20 kDa Bacillus subtilis protein
Buffer:	20 mM Phosphate buffer, 400 mM NaCl, 0.05% NaN3, pH: 6.6
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Oct 3

R_g^Guinier	4.2	nm
D_max	20.0	nm
Volume^Porod	63	nm³

SASDF88 – Delta subunit of RNA polymerase, RNAP (B. subtilis), 800 mM NaCl

Sample:	DNA-directed RNA polymerase subunit delta monomer, 20 kDa Bacillus subtilis protein
Buffer:	20 mM Phosphate buffer, 800 mM NaCl, 0.05% NaN3, pH: 6.6
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Oct 3

R_g^Guinier	4.5	nm
D_max	21.0	nm
Volume^Porod	77	nm³

SASDF98 – Delta subunit of RNA polymerase, RNAP (B. subtilis): Lysine to glutamate mutant, 10mM NaCl

DNA-directed RNA polymerase subunit delta - mutant experimental SAS data

DNA-directed RNA polymerase subunit delta - mutant Kratky plot

Sample:	DNA-directed RNA polymerase subunit delta - mutant monomer, 20 kDa Bacillus subtilis protein
Buffer:	20 mM Phosphate buffer, 10 mM NaCl, 0.05% NaN3, pH: 6.6
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Oct 3

R_g^Guinier	4.3	nm
D_max	19.5	nm
Volume^Porod	58	nm³

SASDFA8 – Delta subunit of RNA polymerase, RNAP (B. subtilis): Lysine to glutamate mutant, 200mM NaCl

Sample:	DNA-directed RNA polymerase subunit delta - mutant monomer, 20 kDa Bacillus subtilis protein
Buffer:	20 mM Phosphate buffer, 200 mM NaCl, 0.05% NaN3, pH: 6.6
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Oct 3

R_g^Guinier	4.6	nm
D_max	22.0	nm
Volume^Porod	76	nm³

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