Small Angle Scattering Biological Data Bank SASBDB

Browse by MACROMOLECULE type: protein

SASDUR8 – Collagenase G at pCa 7

Collagenase ColG (E656D, N659T, G836V, D837Y) experimental SAS data

Sample:	Collagenase ColG (E656D, N659T, G836V, D837Y) monomer, 114 kDa Hathewaya histolytica protein
Buffer:	10 mM HEPES, 100 mM NaCl, 1 mM CaCl2, EDTA, pH: 7.5
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2022 Oct 28

Collagenase G
Cody Brazel

R_g^Guinier	4.8	nm
D_max	18.1	nm
Volume^Porod	182	nm³

SASDYW2 – IgA1 dimer - Control

Human immunoglobulin IgA1 experimental SAS data

Sample:	Human immunoglobulin IgA1 dimer, 320 kDa protein
Buffer:	8.2 mM Na2HPO4, 1.5 mM KH2PO4, 137 mM NaCl, 2.6 mM KCl (PBS), pH: 7.4
Experiment:	SAXS data collected at BM29, ESRF on 2014 Sep 7

Atomistic scattering modelling of the solution structure of human dimeric IgA1 reveals a structural and mechanistic basis for IgA nephropathy. J Biol Chem :113156 (2026)
Bhatt JS, Yeo SC, Ireland SM, Ben-Younis A, Gor J, Molyneux K, Barratt J, Perkins SJ

R_g^Guinier	8.7	nm
D_max	35.0	nm

SASDYX2 – IgA1 dimer - Patient A

Sample:	Human immunoglobulin IgA1 dimer, 320 kDa protein
Buffer:	PBS buffer, 137 mM NaCl, 8.2 mM Na2HPO4, 2.6 mM KCl, 1.5 mM KH2PO4, pH: 7.4
Experiment:	SAXS data collected at BM29, ESRF on 2014 Sep 7

R_g^Guinier	8.6	nm
D_max	35.0	nm

SASDYY2 – IgA1 dimer - Patient B

Sample:	Human immunoglobulin IgA1 dimer, 320 kDa protein
Buffer:	PBS buffer, 137 mM NaCl, 8.2 mM Na2HPO4, 2.6 mM KCl, 1.5 mM KH2PO4, pH: 7.4
Experiment:	SAXS data collected at BM29, ESRF on 2014 Sep 7

R_g^Guinier	8.4	nm
D_max	35.0	nm

SASDYZ2 – IgA1 dimer - Patient C

Sample:	Human immunoglobulin IgA1 dimer, 320 kDa protein
Buffer:	PBS buffer, 137 mM NaCl, 8.2 mM Na2HPO4, 2.6 mM KCl, 1.5 mM KH2PO4, pH: 7.4
Experiment:	SAXS data collected at BM29, ESRF on 2014 Sep 7

R_g^Guinier	8.5	nm
D_max	35.0	nm