Browse by MACROMOLECULE type: protein

SASDDB2 – Phosphorylated Microtubule associated protein MAP2c (isoform 3); 6.8 mg/ml

Microtubule-associated protein 2, isoform 3 experimental SAS data
Microtubule-associated protein 2, isoform 3 Kratky plot
Sample: Microtubule-associated protein 2, isoform 3 monomer, 49 kDa Rattus norvegicus protein
Buffer: 50 mM MOPS, 150 mM NaCl, 0.03% NaN3, pH: 6.9
Experiment: SAXS data collected at BM29, ESRF on 2017 Apr 21
Functionally specific binding regions of microtubule-associated protein 2c exhibit distinct conformations and dynamics. J Biol Chem 293(34):13297-13309 (2018)
Melková K, Zapletal V, Jansen S, Nomilner E, Zachrdla M, Hritz J, Nováček J, Zweckstetter M, Jensen MR, Blackledge M, Žídek L
RgGuinier 7.3 nm

SASDDC2 – Phosphorylated Microtubule associated protein MAP2c (isoform 3); 1.7 mg/ml

Microtubule-associated protein 2, isoform 3 experimental SAS data
Microtubule-associated protein 2, isoform 3 Kratky plot
Sample: Microtubule-associated protein 2, isoform 3 monomer, 49 kDa Rattus norvegicus protein
Buffer: MOPS map2c buffer, pH: 6.9
Experiment: SAXS data collected at BM29, ESRF on 2017 Apr 21
Functionally specific binding regions of microtubule-associated protein 2c exhibit distinct conformations and dynamics. J Biol Chem 293(34):13297-13309 (2018)
Melková K, Zapletal V, Jansen S, Nomilner E, Zachrdla M, Hritz J, Nováček J, Zweckstetter M, Jensen MR, Blackledge M, Žídek L
RgGuinier 6.7 nm

SASDD89 – Structure of Halobacterium salinarum VNG0258H/RosR in the presence of 2 M KCl

VNG0258H/RosR experimental SAS data
DAMMIN model
Sample: VNG0258H/RosR dimer, 29 kDa Halobacterium salinarum NRC-1 protein
Buffer: 50 mM HEPES, 2 M KCl, 0.02% NaN3, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2015 Mar 7
The 3-D structure of VNG0258H/RosR - A haloarchaeal DNA-binding protein in its ionic shell. J Struct Biol (2018)
Kutnowski N, Shmuely H, Dahan I, Shmulevich F, Davidov G, Shahar A, Eichler J, Zarivach R, Shaanan B
RgGuinier 2.3 nm
Dmax 7.5 nm
VolumePorod 58 nm3

SASDD99 – Structure of Halobacterium salinarum VNG0258H/RosR in the presence of 2 M NaCl

VNG0258H/RosR experimental SAS data
DAMMIN model
Sample: VNG0258H/RosR dimer, 29 kDa Halobacterium salinarum NRC-1 protein
Buffer: 50 mM HEPES, 2 M NaCl, 0.02% NaN3, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2015 Mar 7
The 3-D structure of VNG0258H/RosR - A haloarchaeal DNA-binding protein in its ionic shell. J Struct Biol (2018)
Kutnowski N, Shmuely H, Dahan I, Shmulevich F, Davidov G, Shahar A, Eichler J, Zarivach R, Shaanan B
RgGuinier 2.4 nm
Dmax 7.7 nm
VolumePorod 63 nm3

SASDDA9 – Structure of Halobacterium salinarum VNG0258H/RosR in the presence of 2 M KBr

VNG0258H/RosR experimental SAS data
DAMMIN model
Sample: VNG0258H/RosR dimer, 29 kDa Halobacterium salinarum NRC-1 protein
Buffer: 50 mM HEPES, 2 M KBr, 0.02% NaN3, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2015 Mar 7
The 3-D structure of VNG0258H/RosR - A haloarchaeal DNA-binding protein in its ionic shell. J Struct Biol (2018)
Kutnowski N, Shmuely H, Dahan I, Shmulevich F, Davidov G, Shahar A, Eichler J, Zarivach R, Shaanan B
RgGuinier 2.3 nm
Dmax 7.7 nm
VolumePorod 46 nm3

SASDDB9 – Structure of Halobacterium salinarum VNG0258H/RosR in the presence of 2 M NaBr

VNG0258H/RosR experimental SAS data
DAMMIN model
Sample: VNG0258H/RosR dimer, 29 kDa Halobacterium salinarum NRC-1 protein
Buffer: 50 mM HEPES, 2 M NaBr, 0.02% NaN3, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2015 Sep 26
The 3-D structure of VNG0258H/RosR - A haloarchaeal DNA-binding protein in its ionic shell. J Struct Biol (2018)
Kutnowski N, Shmuely H, Dahan I, Shmulevich F, Davidov G, Shahar A, Eichler J, Zarivach R, Shaanan B
RgGuinier 2.5 nm
Dmax 8.1 nm
VolumePorod 59 nm3

SASDDC9 – Structure of Halobacterium salinarum VNG0258H/RosR in the presence of 2 M RbCl

VNG0258H/RosR experimental SAS data
DAMMIN model
Sample: VNG0258H/RosR dimer, 29 kDa Halobacterium salinarum NRC-1 protein
Buffer: 50 mM HEPES, 2 M RbCl, 0.02% NaN3, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2015 Mar 7
The 3-D structure of VNG0258H/RosR - A haloarchaeal DNA-binding protein in its ionic shell. J Struct Biol (2018)
Kutnowski N, Shmuely H, Dahan I, Shmulevich F, Davidov G, Shahar A, Eichler J, Zarivach R, Shaanan B
RgGuinier 3.3 nm
Dmax 9.3 nm
VolumePorod 89 nm3

SASDDW4 – Procollagen lysyl hydroxylase LH3 measured using SEC-SAXS

Procollagen lysyl hydroxylase LH3 experimental SAS data
GASBOR model
Sample: Procollagen lysyl hydroxylase LH3 dimer, 180 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 200 mM NaCl, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2018 Feb 28
Molecular architecture of the multifunctional collagen lysyl hydroxylase and glycosyltransferase LH3. Nat Commun 9(1):3163 (2018)
Scietti L, Chiapparino A, De Giorgi F, Fumagalli M, Khoriauli L, Nergadze S, Basu S, Olieric V, Cucca L, Banushi B, Profumo A, Giulotto E, Gissen P, Forneris F
RgGuinier 5.1 nm
Dmax 21.0 nm
VolumePorod 268 nm3

SASDDB3 – Human mitochondrial cysteine desulfurase (NFS1, ISD11 and Acp heterodimer complex)

Cysteine desulfurase, mitochondrialLYR motif-containing protein 4Acyl carrier protein experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Cysteine desulfurase, mitochondrial dimer, 90 kDa Homo sapiens protein
LYR motif-containing protein 4 dimer, 23 kDa Homo sapiens protein
Acyl carrier protein dimer, 22 kDa Escherichia coli protein
Buffer: 20 mM HEPES, 150 mM NaCl, 5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at Bruker Nanostar, NMRFAM on 2017 May 22
Architectural Features of Human Mitochondrial Cysteine Desulfurase Complexes from Crosslinking Mass Spectrometry and Small-Angle X-Ray Scattering. Structure 26(8):1127-1136.e4 (2018)
Cai K, Frederick RO, Dashti H, Markley JL
RgGuinier 3.7 nm
Dmax 11.8 nm
VolumePorod 204 nm3

SASDDC3 – Human mitochondrial cysteine desulfurase-ISCU (NFS1, ISD11, Acp and ISCU heterodimer complex)

Cysteine desulfurase, mitochondrialLYR motif-containing protein 4Acyl carrier proteinIron-sulfur cluster assembly enzyme ISCU, mitochondrial experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Cysteine desulfurase, mitochondrial dimer, 90 kDa Homo sapiens protein
LYR motif-containing protein 4 dimer, 23 kDa Homo sapiens protein
Acyl carrier protein dimer, 22 kDa Escherichia coli protein
Iron-sulfur cluster assembly enzyme ISCU, mitochondrial dimer, 29 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at Bruker Nanostar, NMRFAM on 2017 May 22
Architectural Features of Human Mitochondrial Cysteine Desulfurase Complexes from Crosslinking Mass Spectrometry and Small-Angle X-Ray Scattering. Structure 26(8):1127-1136.e4 (2018)
Cai K, Frederick RO, Dashti H, Markley JL
RgGuinier 3.9 nm
Dmax 13.7 nm
VolumePorod 218 nm3