Small Angle Scattering Biological Data Bank SASBDB

Browse by MACROMOLECULE type: protein

SASDE33 – Unlabeled nuclear pore complex protein Nup153 (NUL) with denaturant

Nuclear pore complex protein Nup153 experimental SAS data

Unlabeled nuclear pore complex protein Nup153 (NUL) with denaturant Rg histogram

Sample:	Nuclear pore complex protein Nup153 monomer, 12 kDa Homo sapiens protein
Buffer:	PBS, 10 mM DTT, 6 M urea, 0.3 M KCl, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Nov 8

Decoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements. Proc Natl Acad Sci U S A 114(31):E6342-E6351 (2017)
Fuertes G, Banterle N, Ruff KM, Chowdhury A, Mercadante D, Koehler C, Kachala M, Estrada Girona G, Milles S, Mishra A, Onck PR, Gräter F, Esteban-Martín S, Pappu RV, Svergun DI, Lemke EA

R_g^Guinier	3.5	nm
D_max	16.0	nm
Volume^Porod	53	nm³

SASDE43 – Labeled nuclear pore complex protein Nup153 (NUL-Alexa488/Alexa594) with denaturant

Nuclear pore complex protein Nup153Alexa Fluor™ 594 C5 MaleimideAlexa Fluor™ 488 C5 Hydroxylamine experimental SAS data

Nuclear pore complex protein Nup153 Alexa Fluor™ 594 C5 Maleimide Alexa Fluor™ 488 C5 Hydroxylamine Kratky plot

Sample:	Nuclear pore complex protein Nup153 monomer, 12 kDa Homo sapiens protein Alexa Fluor™ 594 C5 Maleimide monomer, 1 kDa Alexa Fluor™ 488 C5 Hydroxylamine monomer, 1 kDa
Buffer:	PBS, 10 mM DTT, 6 M urea, 0.3 M KCl, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Jun 15

R_g^Guinier	3.2	nm
D_max	12.0	nm
Volume^Porod	27	nm³

SASDE53 – Unlabeled dihydrolipoyllysine-residue succinyltransferase component (BBL) with denaturant

Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex experimental SAS data

Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex Kratky plot

Sample:	Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex monomer, 4 kDa Escherichia coli protein
Buffer:	PBS, 10 mM DTT, 6 M urea, 0.3 M KCl, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Nov 8

R_g^Guinier	2.1	nm
D_max	8.3	nm
Volume^Porod	41	nm³

SASDE63 – Labeled dihydrolipoyllysine-residue succinyltransferase component (BBL-Alexa488/Alexa594) with denaturant

Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complexAlexa Fluor™ 594 C5 MaleimideAlexa Fluor™ 488 C5 Hydroxylamine experimental SAS data

Sample:	Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex monomer, 4 kDa Escherichia coli protein Alexa Fluor™ 594 C5 Maleimide monomer, 1 kDa Alexa Fluor™ 488 C5 Hydroxylamine monomer, 1 kDa
Buffer:	PBS, 10 mM DTT, 6 M urea, 0.3 M KCl, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Nov 8

R_g^Guinier	2.3	nm
D_max	10.7	nm
Volume^Porod	13	nm³

SASDE73 – Unlabeled cold shock protein (CSP) with denaturant

Cold shock-like protein experimental SAS data

Sample:	Cold shock-like protein monomer, 7 kDa Thermotoga maritima protein
Buffer:	PBS, 10 mM DTT, 6 M urea, 0.3 M KCl, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Mar 16

R_g^Guinier	2.5	nm
D_max	11.5	nm
Volume^Porod	17	nm³

SASDE83 – Labeled cold shock protein (CSP-Alexa488/Alexa594) with denaturant

Cold shock-like proteinAlexa Fluor™ 594 C5 MaleimideAlexa Fluor™ 488 C5 Hydroxylamine experimental SAS data

Cold shock-like protein Alexa Fluor™ 594 C5 Maleimide Alexa Fluor™ 488 C5 Hydroxylamine Kratky plot

Sample:	Cold shock-like protein monomer, 7 kDa Thermotoga maritima protein Alexa Fluor™ 594 C5 Maleimide monomer, 1 kDa Alexa Fluor™ 488 C5 Hydroxylamine monomer, 1 kDa
Buffer:	PBS, 10 mM DTT, 6 M urea, 0.3 M KCl, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Jun 15

R_g^Guinier	2.2	nm
D_max	8.3	nm
Volume^Porod	9	nm³

SASDE93 – Unlabeled thioredoxin (TRX) with denaturant

Sample:	Thioredoxin 1 monomer, 12 kDa Escherichia coli protein
Buffer:	PBS, 10 mM DTT, 6 M urea, 0.3 M KCl, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Jun 15

R_g^Guinier	3.6	nm
D_max	13.0	nm
Volume^Porod	34	nm³

SASDEA3 – Labeled thioredoxin (TRX-Alexa488/Alexa594) with denaturant

Thioredoxin 1Alexa Fluor™ 594 C5 MaleimideAlexa Fluor™ 488 C5 Hydroxylamine experimental SAS data

Thioredoxin 1 Alexa Fluor™ 594 C5 Maleimide Alexa Fluor™ 488 C5 Hydroxylamine Kratky plot

Sample:	Thioredoxin 1 monomer, 12 kDa Escherichia coli protein Alexa Fluor™ 594 C5 Maleimide monomer, 1 kDa Alexa Fluor™ 488 C5 Hydroxylamine monomer, 1 kDa
Buffer:	PBS, 10 mM DTT, 6 M urea, 0.3 M KCl, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Jun 15

R_g^Guinier	3.2	nm
D_max	13.9	nm
Volume^Porod	31	nm³

SASDEB3 – Unlabeled nuclear pore complex protein Nup98-Nup96 (N98) without denaturant

Nuclear pore complex protein Nup98-Nup96 experimental SAS data

Nuclear pore complex protein Nup98-Nup96 Kratky plot

Sample:	Nuclear pore complex protein Nup98-Nup96 monomer, 15 kDa Homo sapiens protein
Buffer:	PBS, 10 mM DTT, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2015 Jun 24

R_g^Guinier	2.9	nm
D_max	10.4	nm
Volume^Porod	27	nm³

SASDEC3 – Unlabeled nucleoporin NSP1 (NSP) without denaturant

Sample:	Nucleoporin NSP1 monomer, 18 kDa Saccharomyces cerevisiae protein
Buffer:	PBS, 10 mM DTT, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2015 Jun 24

R_g^Guinier	4.1	nm
D_max	15.0	nm
Volume^Porod	45	nm³

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