Browse by MACROMOLECULE type: protein

SASDVF2 – Staphylococcal extracellular adherence protein (Eap) in unbound form

Protein map experimental SAS data
MULTIFOXS model
Sample: Protein map monomer, 50 kDa Staphylococcus aureus (strain … protein
Buffer: 20 mM HEPES, 140 mM NaCl, pH: 7.4
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2023 Jun 3
S. aureus Eap is a polyvalent inhibitor of neutrophil serine proteases. J Biol Chem 300(9):107627 (2024)
Mishra N, Gido CD, Herdendorf TJ, Hammel M, Hura GL, Fu ZQ, Geisbrecht BV
RgGuinier 4.0 nm
VolumePorod 64 nm3

SASDVG2 – Staphylococcal extracellular adherence protein (Eap) bound to human cathepsin-G

Protein mapCathepsin G experimental SAS data
MULTIFOXS model
Sample: Protein map monomer, 50 kDa Staphylococcus aureus (strain … protein
Cathepsin G tetramer, 101 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 140 mM NaCl, pH: 7.4
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2023 Jun 3
S. aureus Eap is a polyvalent inhibitor of neutrophil serine proteases. J Biol Chem 300(9):107627 (2024)
Mishra N, Gido CD, Herdendorf TJ, Hammel M, Hura GL, Fu ZQ, Geisbrecht BV
RgGuinier 5.0 nm
Dmax 20.2 nm
VolumePorod 166 nm3

SASDVH2 – Staphylococcal extracellular adherence protein (Eap) bound to human neutrophil elastase

Protein mapNeutrophil elastase experimental SAS data
MULTIFOXS model
Sample: Protein map monomer, 50 kDa Staphylococcus aureus (strain … protein
Neutrophil elastase tetramer, 93 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 140 mM NaCl, pH: 7.4
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2023 Dec 6
S. aureus Eap is a polyvalent inhibitor of neutrophil serine proteases. J Biol Chem 300(9):107627 (2024)
Mishra N, Gido CD, Herdendorf TJ, Hammel M, Hura GL, Fu ZQ, Geisbrecht BV
RgGuinier 4.1 nm
Dmax 10.6 nm
VolumePorod 152 nm3

SASDVK2 – Core domain of human Cathepsin-G, short truncations to both terminus (Δ22-243Δ253)

Cathepsin G experimental SAS data
PHENIX model
Sample: Cathepsin G monomer, 25 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 140 mM NaCl, pH: 7.4
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2023 Jun 3
S. aureus Eap is a polyvalent inhibitor of neutrophil serine proteases. J Biol Chem 300(9):107627 (2024)
Mishra N, Gido CD, Herdendorf TJ, Hammel M, Hura GL, Fu ZQ, Geisbrecht BV
RgGuinier 1.9 nm
Dmax 6.9 nm
VolumePorod 27 nm3

SASDVL2 – Ternary complex consisted of Human Chatepsin G, Neutrophil elastase and S.aureus MAP domain-containing protein

Cathepsin GMAP domain-containing proteinNeutrophil elastase experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Cathepsin G monomer, 25 kDa Homo sapiens protein
MAP domain-containing protein monomer, 13 kDa Staphylococcus aureus (strain … protein
Neutrophil elastase monomer, 23 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 140 mM NaCl, pH: 7.4
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2023 Jun 3
S. aureus Eap is a polyvalent inhibitor of neutrophil serine proteases. J Biol Chem 300(9):107627 (2024)
Mishra N, Gido CD, Herdendorf TJ, Hammel M, Hura GL, Fu ZQ, Geisbrecht BV
RgGuinier 2.8 nm
Dmax 9.4 nm
VolumePorod 77 nm3

SASDVM2 – Ternary complex consisted of Human Neutrophil elastase tetramer and S.aureus Protein map Eap4-domain-4 (Δ347-476Δ) (Complex: NE/Eap4/NE)

Neutrophil elastaseProtein map experimental SAS data
PHENIX model
Sample: Neutrophil elastase tetramer, 93 kDa Homo sapiens protein
Protein map dimer, 24 kDa Staphylococcus aureus (strain … protein
Buffer: 20 mM HEPES, 140 mM NaCl, pH: 7.4
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2023 Jun 26
S. aureus Eap is a polyvalent inhibitor of neutrophil serine proteases. J Biol Chem 300(9):107627 (2024)
Mishra N, Gido CD, Herdendorf TJ, Hammel M, Hura GL, Fu ZQ, Geisbrecht BV
RgGuinier 3.7 nm
Dmax 11.0 nm
VolumePorod 163 nm3

SASDVN2 – S.aureus Protein Map Eap3 and Eap4 domains (Δ266-476)

Protein map experimental SAS data
MULTIFOXS model
Sample: Protein map monomer, 24 kDa Staphylococcus aureus (strain … protein
Buffer: 20 mM HEPES, 140 mM NaCl, pH: 7.4
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2023 Nov 17
S. aureus Eap is a polyvalent inhibitor of neutrophil serine proteases. J Biol Chem 300(9):107627 (2024)
Mishra N, Gido CD, Herdendorf TJ, Hammel M, Hura GL, Fu ZQ, Geisbrecht BV
RgGuinier 2.5 nm
Dmax 8.5 nm
VolumePorod 28 nm3

SASDVP2 – Multimeric complex cosisted of Human Cathepsin G-Neutrophil elastase tetramers bound to S.aureus Protein Map Eap3 and Eap4 domains

Cathepsin GNeutrophil elastaseEap34 experimental SAS data
BILBOMD model
Sample: Cathepsin G tetramer, 101 kDa Homo sapiens protein
Neutrophil elastase tetramer, 93 kDa Homo sapiens protein
Eap34 dimer, 48 kDa Staphylococcus aureus (strain … protein
Buffer: 20 mM HEPES, 140 mM NaCl, pH: 7.4
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2023 Jun 3
S. aureus Eap is a polyvalent inhibitor of neutrophil serine proteases. J Biol Chem 300(9):107627 (2024)
Mishra N, Gido CD, Herdendorf TJ, Hammel M, Hura GL, Fu ZQ, Geisbrecht BV
RgGuinier 5.4 nm
Dmax 19.4 nm
VolumePorod 500 nm3

SASDUT2 – Recombinant short complement regulator SCR-17/18H (produced in Pichia pastoris with PNGase F treatment)

Complement factor H experimental SAS data
PYMOL model
Sample: Complement factor H monomer, 17 kDa Homo sapiens protein
Buffer: 10 mM HEPES, 137 mM NaCl, pH: 7.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Jan 20
The SCR-17 and SCR-18 glycans in human complement Factor H enhance its regulatory function. J Biol Chem :107624 (2024)
Gao X, Iqbal H, Yu DQ, Gor J, Coker AR, Perkins SJ
RgGuinier 3.1 nm
Dmax 10.5 nm
VolumePorod 46 nm3

SASDUU2 – Recombinant short complement regulator SCR-17/18, without glycan (produced in Escherichia coli)

Complement factor H experimental SAS data
PYMOL model
Sample: Complement factor H monomer, 14 kDa Homo sapiens protein
Buffer: 10 mM HEPES, 137 mM NaCl, pH: 7.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Jan 20
The SCR-17 and SCR-18 glycans in human complement Factor H enhance its regulatory function. J Biol Chem :107624 (2024)
Gao X, Iqbal H, Yu DQ, Gor J, Coker AR, Perkins SJ
RgGuinier 2.3 nm
Dmax 7.0 nm
VolumePorod 19 nm3