Small Angle Scattering Biological Data Bank SASBDB

Browse by MACROMOLECULE type: protein

SASDTJ3 – Teneurin-3 A1B0 isoform in 5 mM EDTA - 0.84 mg/mL

Isoform A1B0 of Teneurin-3 experimental SAS data

Sample:	Isoform A1B0 of Teneurin-3 dimer, 541 kDa Mus musculus protein
Buffer:	20 mM HEPES, 150 mM NaCl, 5 mM EDTA, pH: 7.8
Experiment:	SAXS data collected at BM29, ESRF on 2022 Sep 10

Alternative splicing controls teneurin-3 compact dimer formation for neuronal recognition Nature Communications 15(1) (2024)
Gogou C, Beugelink J, Frias C, Kresik L, Jaroszynska N, Drescher U, Janssen B, Hindges R, Meijer D

R_g^Guinier	8.9	nm
D_max	34.0	nm
Volume^Porod	1049	nm³

SASDT75 – Trypanosoma brucei gambiense invariant surface glycoprotein 43 (ISG43)

T. brucei spp.-specific protein experimental SAS data

Sample:	T. brucei spp.-specific protein monomer, 38 kDa Trypanosoma brucei gambiense … protein
Buffer:	20 mM HEPES, 150 mM NaCl, 3% glycerol, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2022 Sep 8

Beyond the VSG Layer: Exploring the Role of Intrinsic Disorder in the Invariant Surface Glycoproteins of African Trypanosomes. PLoS Pathog 20(4):e1012186 (2024)
Sülzen H, Volkov AN, Geens R, Zahedifard F, Stijlemans B, Zoltner M, Magez S, Sterckx YG, Zoll S

R_g^Guinier	3.2	nm
D_max	11.5	nm
Volume^Porod	53	nm³

SASDT85 – Trypanosoma brucei gambiense invariant surface glycoprotein 64 (ISG64)

64 kDa invariant surface glycoprotein, putative experimental SAS data

Sample:	64 kDa invariant surface glycoprotein, putative monomer, 40 kDa Trypanosoma brucei gambiense … protein
Buffer:	20 mM HEPES, 150 mM NaCl, 3% glycerol, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2022 Sep 8

R_g^Guinier	3.1	nm
D_max	11.2	nm
Volume^Porod	56	nm³

SASDT95 – Trypanosoma brucei gambiense invariant surface glycoprotein 75 (ISG75)

Sample:	ISG75 monomer, 50 kDa Trypanosoma brucei gambiense protein
Buffer:	20 mM HEPES, 150 mM NaCl, 3% glycerol, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2020 Nov 25

R_g^Guinier	3.2	nm
D_max	11.1	nm
Volume^Porod	85	nm³

SASDTA5 – Trypanosoma brucei gambiense variable surface glycoprotein LiTat 1.5

Variable surface glycoprotein LiTat 1.5 experimental SAS data

Sample:	Variable surface glycoprotein LiTat 1.5 dimer, 98 kDa Trypanosoma brucei gambiense protein
Buffer:	50 mM Tris-HCl, 500 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2022 Sep 8

R_g^Guinier	4.4	nm
D_max	17.2	nm
Volume^Porod	170	nm³

SASDTB5 – Trypanosoma brucei gambiense variant surface glycoprotein 3.1

Variant surface glycoprotein 3.1 experimental SAS data

Sample:	Variant surface glycoprotein 3.1 dimer, 103 kDa Trypanosoma brucei gambiense protein
Buffer:	20 mM HEPES, 150 mM NaCl, 3% glycerol, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2022 Sep 8

R_g^Guinier	4.7	nm
D_max	17.0	nm
Volume^Porod	149	nm³

SASDUT6 – Nuclear fusion protein BIK1 in high salt buffer

Nuclear fusion protein BIK1 experimental SAS data

Sample:	Nuclear fusion protein BIK1 dimer, 106 kDa Saccharomyces cerevisiae (strain … protein
Buffer:	20 mM Tris-HCl pH 7.5, 500 mM NaCl, 2% glycerol, 1 mM DTT, pH:
Experiment:	SAXS data collected at B21, Diamond Light Source on 2023 Jul 13

Phase separation of a microtubule plus-end tracking protein into a fluid fractal network (2024)
Czub M, Uliana F, Grubić T, Padeste C, Rosowski K, Dufresne E, Menzel A, Vakonakis I, Gasser U, Steinmetz M

R_g^Guinier	8.8	nm
D_max	28.0	nm
Volume^Porod	467	nm³

SASDUU6 – Nuclear fusion protein BIK1 coiled-coil in a high salt buffer

Sample:	Nuclear fusion protein BIK1 dimer, 55 kDa Saccharomyces cerevisiae (strain … protein
Buffer:	20 mM Tris-HCl pH 7.5, 500 mM NaCl, 2% glycerol, 1 mM DTT, pH:
Experiment:	SAXS data collected at B21, Diamond Light Source on 2023 Jul 13

R_g^Guinier	8.0	nm
D_max	27.0	nm
Volume^Porod	282	nm³

SASDUV6 – Nuclear fusion protein BIK1 ΔQQFF in a high salt buffer

Sample:	Nuclear fusion protein BIK1 dimer, 105 kDa Saccharomyces cerevisiae (strain … protein
Buffer:	20 mM Tris-HCl pH 7.5, 500 mM NaCl, 2% glycerol, 1 mM DTT, pH:
Experiment:	SAXS data collected at B21, Diamond Light Source on 2023 Jul 13

R_g^Guinier	9.4	nm
D_max	29.4	nm
Volume^Porod	632	nm³

SASDS56 – Nucleotide binding domain, NBD (ABC transporter domain) from the RTX toxin ABC transporter RtxB

RTX toxin transporter experimental SAS data

Sample:	RTX toxin transporter monomer, 28 kDa Kingella kingae ATCC … protein
Buffer:	100 mM HEPES, 10 % glycerol, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2023 Mar 20

Type 1 secretion necessitates a tight interplay between all domains of the ABC transporter Scientific Reports 14(1) (2024)
Anlauf M, Bilsing F, Reiners J, Spitz O, Hachani E, Smits S, Schmitt L

R_g^Guinier	2.2	nm
D_max	7.6	nm
Volume^Porod	48	nm³

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