Small Angle Scattering Biological Data Bank SASBDB

Browse by ORGANISM: Escherichia coli

SASDGB3 – DNA-binding protein HU-alpha, E34K mutant bound to 80 bp DNA (pH 4.5)

80bp_DNA Forward80bp_DNA ReverseDNA-binding protein HU-alpha, E34K experimental SAS data

80bp_DNA Forward 80bp_DNA Reverse DNA-binding protein HU-alpha, E34K Kratky plot

Sample:	80bp_DNA Forward monomer, 25 kDa Escherichia coli DNA 80bp_DNA Reverse monomer, 25 kDa Escherichia coli DNA DNA-binding protein HU-alpha, E34K dimer, 19 kDa Escherichia coli protein
Buffer:	10 mM sodium acetate, 50 mM NaCl, pH: 4.5
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Nov 2

Nucleoid remodeling during environmental adaptation is regulated by HU-dependent DNA bundling. Nat Commun 11(1):2905 (2020)
Remesh SG, Verma SC, Chen JH, Ekman AA, Larabell CA, Adhya S, Hammel M

SASDGC3 – DNA-binding protein HU-alpha, E34K mutant bound to 80 bp DNA (pH 5.5)

Sample:	80bp_DNA Forward monomer, 25 kDa Escherichia coli DNA 80bp_DNA Reverse monomer, 25 kDa Escherichia coli DNA DNA-binding protein HU-alpha, E34K dimer, 19 kDa Escherichia coli protein
Buffer:	10 mM Bis-Tris, 50 mM NaCl, pH: 5.5
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Nov 2

SASDGD3 – DNA-binding protein HU-alpha, E34K mutant bound to 80 bp DNA (pH 6.5)

Sample:	80bp_DNA Forward monomer, 25 kDa Escherichia coli DNA 80bp_DNA Reverse monomer, 25 kDa Escherichia coli DNA DNA-binding protein HU-alpha, E34K, 100 kDa Escherichia coli protein
Buffer:	10mM Bis-Tris, 50 mM NaCl, pH: 6.5
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Nov 2

R_g^Guinier	7.3	nm
D_max	28.0	nm
Volume^Porod	330	nm³

SASDGE3 – DNA-binding protein HU-alpha, E34K mutant bound to 80 bp DNA (pH 7.5)

Sample:	80bp_DNA Forward monomer, 25 kDa Escherichia coli DNA 80bp_DNA Reverse monomer, 25 kDa Escherichia coli DNA DNA-binding protein HU-alpha, E34K, 100 kDa Escherichia coli protein
Buffer:	10 mM Bis-Tris, 50 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Nov 2

R_g^Guinier	7.1	nm
D_max	27.5	nm
Volume^Porod	309	nm³

SASDHR9 – GntR-type transcriptional regulator NanR from Escherichia coli

HTH-type transcriptional repressor NanR experimental SAS data

Sample:	HTH-type transcriptional repressor NanR dimer, 59 kDa Escherichia coli protein
Buffer:	20 mM Tris, 150 mM NaCl, 0.1 % (w/v) sodium azide, pH: 8
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2019 Apr 20

Mechanism of NanR gene repression and allosteric induction of bacterial sialic acid metabolism (2020)
Horne C, Venugopal H, Panjikar S, Henrickson A, Brookes E, North R, Murphy J, Friemann R, Griffin M, Ramm G, Demeler B, Dobson R

R_g^Guinier	3.2	nm
D_max	10.5	nm
Volume^Porod	110	nm³

SASDHS9 – GntR-type transcriptional regulator NanR from Escherichia coli in the presence of N-acetylneuraminic acid

Sample:	HTH-type transcriptional repressor NanR dimer, 59 kDa Escherichia coli protein
Buffer:	20 mM Tris, 150 mM NaCl, 20 mM Neu5Ac and 0.1 % (w/v) sodium azide, pH: 8
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2019 Apr 20

R_g^Guinier	3.1	nm
D_max	10.1	nm
Volume^Porod	105	nm³

SASDHT9 – Dimeric NanR-DNA hetero-complex from Escherichia coli

HTH-type transcriptional repressor NanR(GGTATA)2 repeat DNA experimental SAS data

Sample:	HTH-type transcriptional repressor NanR dimer, 59 kDa Escherichia coli protein (GGTATA)2 repeat DNA monomer, 11 kDa DNA
Buffer:	20 mM Tris, 150 mM NaCl, 0.1 % (w/v) sodium azide, pH: 8
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2019 Apr 20

R_g^Guinier	3.3	nm
D_max	9.8	nm
Volume^Porod	108	nm³

SASDFV6 – DNA-binding protein HU-alpha, E38K/V42L double mutant

DNA-binding protein HU-alpha, E38K/V42L double mutant experimental SAS data

Sample:	DNA-binding protein HU-alpha, E38K/V42L double mutant decamer, 95 kDa Escherichia coli protein
Buffer:	50 mM Tris-HCl, 150 mM NaCl, 1 mM DTT, 1 mM PMSF, pH: 7.5
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2015 Apr 23

Nucleoid remodeling during environmental adaptation is regulated by HU-dependent DNA bundling (supplementary)
Soumya G Remesh

R_g^Guinier	3.0	nm
D_max	10.5	nm
Volume^Porod	53	nm³

SASDFW6 – DNA-binding protein HU-alpha, E38K/V42L double mutant bound to 80 bp DNA (ratio DNA:Protein 1:2)

80bp_DNA Forward80bp_DNA ReverseDNA-binding protein HU-alpha, E38K/V42L double mutant experimental SAS data

Sample:	80bp_DNA Forward monomer, 25 kDa Escherichia coli DNA 80bp_DNA Reverse monomer, 25 kDa Escherichia coli DNA DNA-binding protein HU-alpha, E38K/V42L double mutant tetramer, 38 kDa Escherichia coli protein
Buffer:	50 mM Tris-HCl, 150 mM NaCl, 1 mM DTT, 1 mM PMSF, pH: 7.5
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2015 Apr 23

Nucleoid remodeling during environmental adaptation is regulated by HU-dependent DNA bundling (supplementary)
Soumya G Remesh

R_g^Guinier	5.7	nm
D_max	31.3	nm
Volume^Porod	297	nm³

SASDFX6 – DNA-binding protein HU-alpha, E38K/V42L double mutant bound to 80 bp DNA (ratio DNA:Protein 1:4)

Sample:	80bp_DNA Forward monomer, 25 kDa Escherichia coli DNA 80bp_DNA Reverse monomer, 25 kDa Escherichia coli DNA DNA-binding protein HU-alpha, E38K/V42L double mutant tetramer, 38 kDa Escherichia coli protein
Buffer:	50 mM Tris-HCl, 150 mM NaCl, 1 mM DTT, 1 mM PMSF, pH: 7.5
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2015 Apr 23

Nucleoid remodeling during environmental adaptation is regulated by HU-dependent DNA bundling (supplementary)
Soumya G Remesh

R_g^Guinier	5.7	nm
D_max	25.7	nm
Volume^Porod	195	nm³

First << Prev Page 17 of 28 Next >> Last