Small Angle Scattering Biological Data Bank SASBDB

SASDW69 – β-Lactoglobulin A at 87.5 μM (pH 3.0)

Beta-lactoglobulin experimental SAS data

Sample:	Beta-lactoglobulin , 20 kDa Bos taurus protein
Buffer:	20 mM citrate, 100 mM NaCl, 1 mM TCEP, pH:
Experiment:	SAXS data collected at BM29, ESRF on 2021 Dec 3

KDSAXS: A tool for Analyzing Binding Equilibria with SAXS Data using Explicit Models Journal of Molecular Biology :169103 (2025)
Gomes T, Ruiz L, Martin-Malpartida P, Bernadó P, Baptista A, Macias M, Cordeiro T

R_g^Guinier	2.2	nm
D_max	7.5	nm
Volume^Porod	53	nm³

SASDW79 – β-Lactoglobulin A at 116.3 μM (pH 3.0)

Sample:	Beta-lactoglobulin , 20 kDa Bos taurus protein
Buffer:	20 mM citrate, 100 mM NaCl, 1 mM TCEP, pH:
Experiment:	SAXS data collected at BM29, ESRF on 2021 Dec 3

R_g^Guinier	2.2	nm
D_max	8.5	nm
Volume^Porod	51	nm³

SASDW89 – Proliferating Cell Nuclear Antigen (PCNA) sliding clamp

Proliferating cell nuclear antigen experimental SAS data

Proliferating cell nuclear antigen Kratky plot

Sample:	Proliferating cell nuclear antigen trimer, 87 kDa Homo sapiens protein
Buffer:	137 mM NaCl, 2.7 mM KCl, 10 mM sodium phosphate, 2 mM potassium phosphate (PBS), pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2012 Apr 23

R_g^Guinier	3.4	nm
D_max	9.0	nm
Volume^Porod	137	nm³

SASDW99 – Proliferating cell nuclear antigen:PCNA-associated factor (PCNA:P15) titration, 150 µM:30 µM

Proliferating cell nuclear antigenPCNA-associated factor experimental SAS data

Proliferating cell nuclear antigen PCNA-associated factor Kratky plot

Sample:	Proliferating cell nuclear antigen trimer, 87 kDa Homo sapiens protein PCNA-associated factor monomer, 12 kDa Homo sapiens protein
Buffer:	137 mM NaCl, 2.7 mM KCl, 10 mM sodium phosphate, 2 mM potassium phosphate (PBS), pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2012 Apr 23

R_g^Guinier	3.5	nm
D_max	9.7	nm
Volume^Porod	128	nm³

SASDWA9 – Proliferating cell nuclear antigen:PCNA-associated factor (PCNA:P15) titration, 150 µM:60 µM

Sample:	Proliferating cell nuclear antigen trimer, 87 kDa Homo sapiens protein PCNA-associated factor monomer, 12 kDa Homo sapiens protein
Buffer:	137 mM NaCl, 2.7 mM KCl, 10 mM sodium phosphate, 2 mM potassium phosphate (PBS), pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2012 Apr 23

R_g^Guinier	3.5	nm
D_max	10.0	nm
Volume^Porod	158	nm³

SASDWB9 – Proliferating cell nuclear antigen:PCNA-associated factor (PCNA:P15) titration, 150 µM:90 µM

Sample:	Proliferating cell nuclear antigen trimer, 87 kDa Homo sapiens protein PCNA-associated factor monomer, 12 kDa Homo sapiens protein
Buffer:	137 mM NaCl, 2.7 mM KCl, 10 mM sodium phosphate, 2 mM potassium phosphate (PBS), pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2012 Apr 23

R_g^Guinier	3.7	nm
D_max	11.0	nm
Volume^Porod	184	nm³

SASDWC9 – Proliferating cell nuclear antigen:PCNA-associated factor (PCNA:P15) titration, 150 µM:150 µM

Sample:	Proliferating cell nuclear antigen trimer, 87 kDa Homo sapiens protein PCNA-associated factor monomer, 12 kDa Homo sapiens protein
Buffer:	137 mM NaCl, 2.7 mM KCl, 10 mM sodium phosphate, 2 mM potassium phosphate (PBS), pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2012 Apr 23

R_g^Guinier	3.7	nm
D_max	11.0	nm
Volume^Porod	219	nm³

SASDWD9 – Proliferating cell nuclear antigen:PCNA-associated factor (PCNA:P15) titration, 150 µM:200 µM

Sample:	Proliferating cell nuclear antigen trimer, 87 kDa Homo sapiens protein PCNA-associated factor monomer, 12 kDa Homo sapiens protein
Buffer:	137 mM NaCl, 2.7 mM KCl, 10 mM sodium phosphate, 2 mM potassium phosphate (PBS), pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2012 Apr 23

R_g^Guinier	3.7	nm
D_max	11.0	nm
Volume^Porod	208	nm³

SASDWE9 – Proliferating cell nuclear antigen:PCNA-associated factor (PCNA:P15) titration, 150 µM:300 µM

Sample:	Proliferating cell nuclear antigen trimer, 87 kDa Homo sapiens protein PCNA-associated factor monomer, 12 kDa Homo sapiens protein
Buffer:	137 mM NaCl, 2.7 mM KCl, 10 mM sodium phosphate, 2 mM potassium phosphate (PBS), pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2012 Apr 23

R_g^Guinier	3.9	nm
D_max	11.5	nm
Volume^Porod	194	nm³

SASDWF9 – Proliferating cell nuclear antigen:PCNA-associated factor (PCNA:P15) titration, 150 µM:370 µM

Sample:	Proliferating cell nuclear antigen trimer, 87 kDa Homo sapiens protein PCNA-associated factor monomer, 12 kDa Homo sapiens protein
Buffer:	137 mM NaCl, 2.7 mM KCl, 10 mM sodium phosphate, 2 mM potassium phosphate (PBS), pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2012 Apr 23

R_g^Guinier	3.7	nm
D_max	11.0	nm
Volume^Porod	187	nm³

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