Small Angle Scattering Biological Data Bank SASBDB

SASDM75 – Ras-like protein 1 guanine nucleotide exchange factor region of Candida albicans cell division control protein 25

Cell division control protein 25 experimental SAS data

Sample:	Cell division control protein 25 monomer, 55 kDa Candida albicans (strain … protein
Buffer:	20 mM Na-phosphate, 150 mM NaCl, 5% glycerol, 3 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Jun 21

Pathogen-specific structural features of Candida albicans Ras1 activation complex: uncovering new antifungal drug targets. mBio :e0063823 (2023)
Manso JA, Carabias A, Sárkány Z, de Pereda JM, Pereira PJB, Macedo-Ribeiro S

R_g^Guinier	3.2	nm
D_max	11.8	nm
Volume^Porod	81	nm³

SASDM85 – GTP-binding domain of Candida albicans Ras-like protein 1 in complex with the guanine nucleotide exchange factor region of cell division control protein 25

GTP-binding domain of Ras-like protein 1Cell division control protein 25 experimental SAS data

Sample:	GTP-binding domain of Ras-like protein 1 monomer, 19 kDa protein Cell division control protein 25 monomer, 55 kDa Candida albicans (strain … protein
Buffer:	20 mM Na-phosphate, 150 mM NaCl, 1 mM EDTA, 5% glycerol, 3 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Jun 21

R_g^Guinier	3.2	nm
D_max	11.8	nm
Volume^Porod	99	nm³

SASDM95 – Candida albicans Ras-like protein 1 in complex with the guanine nucleotide exchange factor region of cell division control protein 25

Ras-like protein 1Cell division control protein 25 experimental SAS data

Candida albicans Ras-like protein 1 in complex with the guanine nucleotide exchange factor region of cell division control protein 25 Rg histogram

Sample:	Ras-like protein 1 monomer, 33 kDa Candida albicans protein Cell division control protein 25 monomer, 55 kDa Candida albicans (strain … protein
Buffer:	20 mM Na-phosphate, 150 mM NaCl, 1 mM EDTA, 5% glycerol, 3 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Jun 21

R_g^Guinier	4.0	nm
D_max	17.7	nm
Volume^Porod	149	nm³

SASDSY6 – Outer membrane protein MIP (LpMIP) apo state

Outer membrane protein MIP experimental SAS data

Sample:	Outer membrane protein MIP dimer, 46 kDa Legionella pneumophila subsp. … protein
Buffer:	20 mM Tris-HCl, 150 mM NaCl, 10 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Mar 16

Legionella pneumophila macrophage infectivity potentiator protein appendage domains modulate protein dynamics and inhibitor binding International Journal of Biological Macromolecules :126366 (2023)
Wiedemann C, Whittaker J, Carrillo V, Goretzki B, Dajka M, Tebbe F, Harder J, Krajczy P, Joseph B, Hausch F, Guskov A, Hellmich U

R_g^Guinier	3.1	nm
D_max	10.0	nm
Volume^Porod	66	nm³

SASDSZ6 – Outer membrane protein MIP (LpMIP) with bound inhibitor JK095

Sample:	Outer membrane protein MIP dimer, 46 kDa Legionella pneumophila subsp. … protein
Buffer:	20 mM Tris-HCl, 150 mM NaCl, 10 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Mar 16

R_g^Guinier	3.1	nm
D_max	10.2	nm
Volume^Porod	65	nm³

SASDS27 – Outer membrane protein MIP (LpMIP) with bound inhibitor JK236

Sample:	Outer membrane protein MIP dimer, 46 kDa Legionella pneumophila subsp. … protein
Buffer:	20 mM Tris-HCl, 150 mM NaCl, 10 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Mar 16

R_g^Guinier	3.1	nm
D_max	9.4	nm
Volume^Porod	63	nm³

SASDPB5 – SAXS curve of RNA Xist A-repeats (Liu buffer)

Xist A-repeat lncRNA experimental SAS data

Sample:	Xist A-repeat lncRNA monomer, 148 kDa Homo sapiens RNA
Buffer:	25 mM K-HEPES, 0.1 mM Na-EDTA, 150 mM KCl, 15 mM MgCl2, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Jun 20

Conformation and structural dynamics of the Xist lncRNA A-repeats (2023)
Jones A, Gabel F, Bohn S, Wolfe G, Sattler M

R_g^Guinier	12.1	nm
D_max	45.0	nm

SASDPC5 – SAXS curve of RNA Xist A-repeats (Smola buffer)

Sample:	Xist A-repeat lncRNA monomer, 148 kDa Homo sapiens RNA
Buffer:	100 mM HEPES, 100 mM NaCl, 10 mM MgCl2, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Jun 20

Conformation and structural dynamics of the Xist lncRNA A-repeats (2023)
Jones A, Gabel F, Bohn S, Wolfe G, Sattler M

R_g^Guinier	9.8	nm
D_max	40.0	nm
Volume^Porod	657	nm³

SASDPD5 – SAXS curve of RNA Xist A-repeats (Maenner buffer)

Sample:	Xist A-repeat lncRNA monomer, 148 kDa Homo sapiens RNA
Buffer:	20 mM HEPES-KOH, 100 mM KCl, 0.2 mM EDTA, 0.5 mM DTT, 0.5 mM PMSF, 20% glycerol, 3.25 mM MgCl2, pH: 7.9
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Jun 20

Conformation and structural dynamics of the Xist lncRNA A-repeats (2023)
Jones A, Gabel F, Bohn S, Wolfe G, Sattler M

R_g^Guinier	8.9	nm
D_max	35.0	nm
Volume^Porod	414	nm³

SASDPE5 – SAXS curve of RNA Xist A-repeats (Duszczyk buffer)

Sample:	Xist A-repeat lncRNA monomer, 148 kDa Homo sapiens RNA
Buffer:	10 mM NaH2PO4/Na2HPO4, 100 mM NaCl, 0.02 mM EDTA, 0.02% azide, pH: 6
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Jun 20

Conformation and structural dynamics of the Xist lncRNA A-repeats (2023)
Jones A, Gabel F, Bohn S, Wolfe G, Sattler M

R_g^Guinier	7.6	nm
D_max	30.0	nm
Volume^Porod	370	nm³

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