Small Angle Scattering Biological Data Bank SASBDB

SASDMC4 – Plasmodium falciparum tyrosyl-tRNA synthetase

Tyrosine--tRNA ligase experimental SAS data

Sample:	Tyrosine--tRNA ligase dimer, 87 kDa Plasmodium falciparum (isolate … protein
Buffer:	20 mM Tris-HCl, 150 mM NaCl, 0.5 mM TCEP, 0.1% sodium azide, pH: 7.4
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2021 Jun 4

Reaction hijacking of tyrosine tRNA synthetase as a new whole-of-life-cycle antimalarial strategy Science 376(6597):1074-1079 (2022)
Xie S, Metcalfe R, Dunn E, Morton C, Huang S, Puhalovich T, Du Y, Wittlin S, Nie S, Luth M, Ma L, Kim M, Pasaje C, Kumpornsin K, Giannangelo C, Houghton F, Churchyard A, Famodimu M, Barry D, Gillett D...

R_g^Guinier	3.7	nm
D_max	12.1	nm
Volume^Porod	126	nm³

SASDMD4 – Plasmodium falciparum tyrosyl-tRNA synthetase, S234C mutant

Tyrosine--tRNA ligase (S234C) experimental SAS data

Sample:	Tyrosine--tRNA ligase (S234C) dimer, 87 kDa Plasmodium falciparum (isolate … protein
Buffer:	20 mM Tris-HCl, 150 mM NaCl, 0.5 mM TCEP, 0.1% sodium azide, pH: 7.4
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2021 Jun 4

R_g^Guinier	3.7	nm
D_max	12.0	nm
Volume^Porod	124	nm³

SASDME4 – Human tyrosyl tRNA synthetase

Tyrosine--tRNA ligase, cytoplasmic experimental SAS data

Sample:	Tyrosine--tRNA ligase, cytoplasmic dimer, 82 kDa Homo sapiens protein
Buffer:	20 mM Tris-HCl, 150 mM NaCl, 0.5 mM TCEP, 0.1% sodium azide, pH: 7.4
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2021 Jun 4

R_g^Guinier	3.6	nm
D_max	11.5	nm
Volume^Porod	118	nm³

SASDNB7 – C-terminal domain of pUL21 with N-terminal His-tag

Tegument protein UL21 (C-terminal domain) experimental SAS data

Sample:	Tegument protein UL21 (C-terminal domain) monomer, 29 kDa Human herpesvirus 1 … protein
Buffer:	20 mM Tris pH 8.5, 500 mM NaCl, 3% (v/v) glycerol, 1 mM DTT, pH: 8.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Sep 25

Herpes simplex virus 1 protein pUL21 alters ceramide metabolism by activating the inter-organelle transport protein CERT Journal of Biological Chemistry :102589 (2022)
Benedyk T, Connor V, Caroe E, Shamin M, Svergun D, Deane J, Jeffries C, Crump C, Graham S

R_g^Guinier	2.2	nm
D_max	8.5	nm
Volume^Porod	49	nm³

SASDNC7 – miniCERTL with N-terminal His-tag

Ceramide transfer protein (recombinant CERTL: amino acids 20-130 and 351-624) experimental SAS data

Sample:	Ceramide transfer protein (recombinant CERTL: amino acids 20-130 and 351-624) monomer, 45 kDa Homo sapiens protein
Buffer:	20 mM Tris pH 8.5, 500 mM NaCl, 3% (v/v) glycerol, 1 mM DTT, pH: 8.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Sep 25

R_g^Guinier	2.7	nm
D_max	9.1	nm
Volume^Porod	71	nm³

SASDND7 – Complex of H6-pUL21C and H6-miniCERTL

Tegument protein UL21 (C-terminal domain)Ceramide transfer protein (recombinant CERTL: amino acids 20-130 and 351-624) experimental SAS data

Sample:	Tegument protein UL21 (C-terminal domain) monomer, 29 kDa Human herpesvirus 1 … protein Ceramide transfer protein (recombinant CERTL: amino acids 20-130 and 351-624) monomer, 45 kDa Homo sapiens protein
Buffer:	20 mM Tris pH 8.5, 500 mM NaCl, 3% (v/v) glycerol, 1 mM DTT, pH: 8.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Sep 25

R_g^Guinier	3.3	nm
D_max	13.6	nm
Volume^Porod	84	nm³

SASDNE7 – Complex of H6-miniCERTL and full-length pUL21-H6

Tegument protein UL21Ceramide transfer protein (recombinant CERTL: amino acids 20-130 and 351-624) experimental SAS data

Sample:	Tegument protein UL21 monomer, 59 kDa Human alphaherpesvirus 1 … protein Ceramide transfer protein (recombinant CERTL: amino acids 20-130 and 351-624) monomer, 45 kDa Homo sapiens protein
Buffer:	20 mM Tris pH 8.5, 500 mM NaCl, 3% (v/v) glycerol, 1 mM DTT, pH: 8.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Sep 25

R_g^Guinier	4.1	nm
D_max	15.8	nm
Volume^Porod	158	nm³

SASDKZ9 – Transcription elongation factor SPT6 - ΔN Spt6

Transcription elongation factor SPT6 - ΔN Spt6 variant experimental SAS data

Transcription elongation factor SPT6 - ΔN Spt6 Rg histogram

Sample:	Transcription elongation factor SPT6 - ΔN Spt6 variant monomer, 132 kDa Saccharomyces cerevisiae (strain … protein
Buffer:	25 mM Hepes; 150 NaCl; 0.5 mM EDTA; 5% glycerol; 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Oct 3

Cooperation between intrinsically disordered and ordered regions of Spt6 regulates nucleosome and Pol II CTD binding, and nucleosome assembly. Nucleic Acids Res (2022)
Kasiliauskaite A, Kubicek K, Klumpler T, Zanova M, Zapletal D, Koutna E, Novacek J, Stefl R

R_g^Guinier	4.7	nm
D_max	14.4	nm
Volume^Porod	288	nm³

SASDL22 – Transcription elongation factor SPT6 - ΔtSH2 variant

Transcription elongation factor SPT6 - ΔtSH2 variant experimental SAS data

Transcription elongation factor SPT6 - ΔtSH2 variant Rg histogram

Sample:	Transcription elongation factor SPT6 - ΔtSH2 variant monomer, 122 kDa Saccharomyces cerevisiae (strain … protein
Buffer:	25 mM NaPi; 150mM NaCl; 0.5 mM EDTA; 5% glycerol; 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2015 Oct 22

R_g^Guinier	4.1	nm
D_max	13.9	nm
Volume^Porod	245	nm³

SASDL32 – Transcription elongation factor SPT6

Transcription elongation factor SPT6 experimental SAS data

Transcription elongation factor SPT6 Rg histogram

Sample:	Transcription elongation factor SPT6 monomer, 145 kDa Saccharomyces cerevisiae (strain … protein
Buffer:	25 mM NaPi; 150mM NaCl; 0.5 mM EDTA; 5% glycerol; 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2015 Oct 22

R_g^Guinier	4.5	nm
D_max	12.0	nm
Volume^Porod	249	nm³

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