Small Angle Scattering Biological Data Bank SASBDB

SASDWZ4 – Shewanella benthica hemoglobin K111I 200 MPa

Group 1 truncated hemoglobin (C51S, C71S, K111I) experimental SAS data

Group 1 truncated hemoglobin (C51S, C71S, K111I) Kratky plot

Sample:	Group 1 truncated hemoglobin (C51S, C71S, K111I) , 13 kDa Shewanella benthica KT99 protein
Buffer:	14 mM Tris, 6 mM potassium phosphate, pH: 7
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Feb 18

Extremophilic hemoglobins: The structure of Shewanella benthica truncated hemoglobin N Journal of Biological Chemistry :108223 (2025)
Martinez Grundman J, Schultz T, Schlessman J, Johnson E, Gillilan R, Lecomte J

R_g^Guinier	1.7	nm
D_max	6.8	nm
Volume^Porod	24	nm³

SASDW25 – Shewanella benthica hemoglobin K111I 250 MPa

Sample:	Group 1 truncated hemoglobin (C51S, C71S, K111I) , 13 kDa Shewanella benthica KT99 protein
Buffer:	14 mM Tris, 6 mM potassium phosphate, pH: 7
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Feb 18

R_g^Guinier	1.8	nm
D_max	7.0	nm
Volume^Porod	29	nm³

SASDW35 – Shewanella benthica hemoglobin K111I 300 MPa

Sample:	Group 1 truncated hemoglobin (C51S, C71S, K111I) , 13 kDa Shewanella benthica KT99 protein
Buffer:	14 mM Tris, 6 mM potassium phosphate, pH: 7
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Feb 18

R_g^Guinier	1.8	nm
D_max	9.0	nm
Volume^Porod	28	nm³

SASDW45 – Shewanella benthica hemoglobin K111I 350 MPa

Sample:	Group 1 truncated hemoglobin (C51S, C71S, K111I) , 13 kDa Shewanella benthica KT99 protein
Buffer:	14 mM Tris, 6 mM potassium phosphate, pH: 7
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Feb 18

R_g^Guinier	1.9	nm
D_max	10.0	nm
Volume^Porod	29	nm³

SASDW55 – Shewanella benthica hemoglobin Y34F 0.101 MPa

Group 1 truncated hemoglobin (Y34F, C51S, C71S) experimental SAS data

Group 1 truncated hemoglobin (Y34F, C51S, C71S) Kratky plot

Sample:	Group 1 truncated hemoglobin (Y34F, C51S, C71S) , 13 kDa Shewanella benthica KT99 protein
Buffer:	14 mM Tris, 6 mM potassium phosphate, pH: 7
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Feb 18

R_g^Guinier	2.3	nm
D_max	6.9	nm
Volume^Porod	75	nm³

SASDW65 – Shewanella benthica hemoglobin Y34F 100 MPa

Sample:	Group 1 truncated hemoglobin (Y34F, C51S, C71S) , 13 kDa Shewanella benthica KT99 protein
Buffer:	14 mM Tris, 6 mM potassium phosphate, pH: 7
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Feb 18

R_g^Guinier	2.3	nm
D_max	7.0	nm
Volume^Porod	66	nm³

SASDW75 – Shewanella benthica hemoglobin Y34F 150 MPa

Sample:	Group 1 truncated hemoglobin (Y34F, C51S, C71S) , 13 kDa Shewanella benthica KT99 protein
Buffer:	14 mM Tris, 6 mM potassium phosphate, pH: 7
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Feb 18

R_g^Guinier	2.2	nm
D_max	7.0	nm
Volume^Porod	61	nm³

SASDW85 – Shewanella benthica hemoglobin Y34F 200 MPa

Sample:	Group 1 truncated hemoglobin (Y34F, C51S, C71S) , 13 kDa Shewanella benthica KT99 protein
Buffer:	14 mM Tris, 6 mM potassium phosphate, pH: 7
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Feb 18

R_g^Guinier	2.0	nm
D_max	8.5	nm
Volume^Porod	24	nm³

SASDW95 – Shewanella benthica hemoglobin Y34F 250 MPa

Sample:	Group 1 truncated hemoglobin (Y34F, C51S, C71S) , 13 kDa Shewanella benthica KT99 protein
Buffer:	14 mM Tris, 6 mM potassium phosphate, pH: 7
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Feb 18

R_g^Guinier	2.1	nm
D_max	6.6	nm
Volume^Porod	49	nm³

SASDWA5 – Shewanella benthica hemoglobin Y108A 0.101 MPa

Group 1 truncated hemoglobin (C51S, C71S, Y108A) experimental SAS data

Group 1 truncated hemoglobin (C51S, C71S, Y108A) Kratky plot

Sample:	Group 1 truncated hemoglobin (C51S, C71S, Y108A) , 13 kDa Shewanella benthica KT99 protein
Buffer:	14 mM Tris, 6 mM potassium phosphate, pH: 7
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Feb 18

R_g^Guinier	1.4	nm
D_max	4.0	nm
Volume^Porod	20	nm³

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