SASDCK6 – Sheath tail protein (DSY3957) from Desulfitobacterium hafniense, Northeast Structural Genomics Consortium Target DhR18

Uncharacterized protein experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Uncharacterized protein monomer, 48 kDa Desulfitobacterium hafniense protein
Buffer: 5 mM DTT 100 mM NaCl 10 mM Tris-HCl 0.02 % NaN3, pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2010 Feb 12
Small angle X-ray scattering as a complementary tool for high-throughput structural studies. Biopolymers 95(8):517-30 (2011)
Grant TD, Luft JR, Wolfley JR, Tsuruta H, Martel A, Montelione GT, Snell EH
RgGuinier 2.8 nm
Dmax 9.9 nm
VolumePorod 66 nm3

SASDAG5 – RNA shaperone Hfq

RNA chaperone Hfq experimental SAS data
DAMMIN model
Sample: RNA chaperone Hfq hexamer, 67 kDa Escherichia coli protein
Buffer: 50 mM Tris-HCL 150 mM NaCl 1.0 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2008 May 2
Structural insights into the dynamics and function of the C-terminus of the E. coli RNA chaperone Hfq. Nucleic Acids Res 39(11):4900-15 (2011)
Beich-Frandsen M, Vecerek B, Konarev PV, Sjöblom B, Kloiber K, Hämmerle H, Rajkowitsch L, Miles AJ, Kontaxis G, Wallace BA, Svergun DI, Konrat R, Bläsi U, Djinovic-Carugo K
RgGuinier 3.2 nm
Dmax 11.2 nm
VolumePorod 110 nm3

SASDAS6 – Plectin, fragment of the plakin domain encompassing the spectrin repeats SR3-SR4-SR5 and the SH3

Plectin experimental SAS data
DAMMIF model
Sample: Plectin monomer, 43 kDa Homo sapiens protein
Buffer: 20 mM Sodium Phosphate 150 mM NaCl 5% glycerol 2.5 mM DTT, pH: 7.5
Experiment: SAXS data collected at cSAXS, Swiss Light Source on 2009 Jul 24
The structure of the plakin domain of plectin reveals a non-canonical SH3 domain interacting with its fourth spectrin repeat. J Biol Chem 286(14):12429-38 (2011)
Ortega E, Buey RM, Sonnenberg A, de Pereda JM
RgGuinier 4.4 nm
Dmax 14.5 nm
VolumePorod 57 nm3

SASDNU2 – Homodimerization of a membrane type 1 matrix metalloproteinase (MT1-MMP)

HemopexinHemopexin experimental SAS data
CUSTOM IN-HOUSE model
Sample: Hemopexin monomer, 23 kDa Homo sapiens protein
Hemopexin dimer, 46 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 150 mM NaCl, 10 mM CaCl2, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2006 Nov 25
The Dimer Interface of the Membrane Type 1 Matrix Metalloproteinase Hemopexin Domain Journal of Biological Chemistry 286(9):7587-7600 (2011)
Tochowicz A, Goettig P, Evans R, Visse R, Shitomi Y, Palmisano R, Ito N, Richter K, Maskos K, Franke D, Svergun D, Nagase H, Bode W, Itoh Y
RgGuinier 2.3 nm
Dmax 8.0 nm
VolumePorod 48 nm3

SASDLR5 – Multidomain Regulatory Protein Rv1364c (monomer)

Multidomain regulatory protein Rv1364c experimental SAS data
GASBOR model
Sample: Multidomain regulatory protein Rv1364c monomer, 70 kDa Mycobacterium tuberculosis (strain … protein
Buffer: 25 mM Tris-HCl, 50 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2007 Feb 14
Structural characterization of the multidomain regulatory protein Rv1364c from Mycobacterium tuberculosis. Structure 19(1):56-69 (2011)
King-Scott J, Konarev PV, Panjikar S, Jordanova R, Svergun DI, Tucker PA
RgGuinier 3.8 nm
Dmax 14.0 nm
VolumePorod 130 nm3

SASDLS5 – Multidomain Regulatory Protein Rv1364c (dimer)

Multidomain regulatory protein Rv1364c experimental SAS data
GASBOR model
Sample: Multidomain regulatory protein Rv1364c dimer, 139 kDa Mycobacterium tuberculosis (strain … protein
Buffer: 25 mM Tris-HCl, 50 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2007 Feb 14
Structural characterization of the multidomain regulatory protein Rv1364c from Mycobacterium tuberculosis. Structure 19(1):56-69 (2011)
King-Scott J, Konarev PV, Panjikar S, Jordanova R, Svergun DI, Tucker PA
RgGuinier 4.5 nm
Dmax 18.0 nm
VolumePorod 289 nm3

SASDLF3 – Staphylococcal immunoglobulin-binding protein bound to complement component C3

Protein A experimental SAS data
DAMMIN model
Sample: Protein A monomer, 28 kDa Staphylococcus aureus protein
Buffer: 10 mM HEPES, pH 7.2, 3 mM EDTA, pH: 7.2
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2005 May 12
A structural basis for Staphylococcal complement subversion: X-ray structure of the complement-binding domain of Staphylococcus aureus protein Sbi in complex with ligand C3d Molecular Immunology 48(4):452-462 (2011)
Clark E, Crennell S, Upadhyay A, Zozulya A, Mackay J, Svergun D, Bagby S, van den Elsen J
RgGuinier 4.6 nm
Dmax 16.0 nm
VolumePorod 81 nm3

SASDAC5 – ImportinA_ImportinB

ImportinA_ImportinB experimental SAS data
DAMMIF model
Sample: ImportinA_ImportinB monomer, 160 kDa Escherichia coli protein
Buffer: 50 mM Tris-HCL 150 mM NaCl 1.0 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2009 Oct 29
Recognition of nucleoplasmin by its nuclear transport receptor importin α/β: insights into a complete import complex. Biochemistry 49(45):9756-69 (2010)
Falces J, Arregi I, Konarev PV, Urbaneja MA, Svergun DI, Taneva SG, Bañuelos S
RgGuinier 5.7 nm
Dmax 19.0 nm
VolumePorod 390 nm3

SASDAD5 – Nucleoplasmin_ImpA_ImpB

Nucleoplasmin_importinA_importinB experimental SAS data
DAMMIF model
Sample: Nucleoplasmin_importinA_importinB , 900 kDa Escherichia coli protein
Buffer: 50 mM Tris-HCL 150 mM NaCl 1.0 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2008 May 23
Recognition of nucleoplasmin by its nuclear transport receptor importin α/β: insights into a complete import complex. Biochemistry 49(45):9756-69 (2010)
Falces J, Arregi I, Konarev PV, Urbaneja MA, Svergun DI, Taneva SG, Bañuelos S
RgGuinier 8.6 nm
Dmax 28.0 nm
VolumePorod 1800 nm3

SASDAV6 – Cysteine desulfurase IscS dimer

Cysteine desulfurase IscS experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Cysteine desulfurase IscS dimer, 87 kDa Escherichia coli protein
Buffer: 20 mM Tris-HCl 150 mM NaCl 10 mM β-mercaptoethanol, pH: 8
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2009 Nov 5
Structural bases for the interaction of frataxin with the central components of iron-sulphur cluster assembly. Nat Commun 1:95 (2010)
Prischi F, Konarev PV, Iannuzzi C, Pastore C, Adinolfi S, Martin SR, Svergun DI, Pastore A
RgGuinier 3.2 nm
Dmax 10.9 nm

4692 hits found.