Small angle X-ray scattering as a complementary tool for high-throughput structural studies.

Grant TD, Luft JR, Wolfley JR, Tsuruta H, Martel A, Montelione GT, Snell EH
Biopolymers 95(8):517-30 (2011 Aug)
PMID: 21462184
doi: 10.1002/bip.21630 		Submitted to SASBDB: 2017 Jul 12
Published in SASBDB:

SASDC96 – N-terminal domain of Diguanylate cyclase with PAS/PAC sensor (Maqu_2914) from Marinobacter aquaeolei, Northeast Structural Genomics Consortium Target MqR66C

Diguanylate cyclase with PAS/PAC sensor experimental SAS data
DAMFILT model
Sample: Diguanylate cyclase with PAS/PAC sensor dimer, 27 kDa Marinobacter hydrocarbonoclasticus protein
Buffer: 5 mM DTT 100 mM NaCl 10 mM Tris-HCl 0.02 % NaN3, pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2010 Feb 12
RgGuinier 2.0 nm
Dmax 6.7 nm
VolumePorod 41 nm3

SASDCA6 – SirA-like protein (DSY4693) from Desulfitobacterium hafniense, Northeast Structural Genomics Consortium Target DhR2A

Uncharacterized protein experimental SAS data
DAMFILT model
Sample: Uncharacterized protein monomer, 9 kDa Desulfitobacterium hafniense protein
Buffer: 5 mM DTT 100 mM NaCl 10 mM Tris-HCl 0.02 % NaN3, pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2010 Feb 12
RgGuinier 1.5 nm
Dmax 5.3 nm
VolumePorod 13 nm3

SASDCB6 – Nmul_A1745 protein from Nitrosospira multiformis, Northeast Structural Genomics Consortium Target NmR72

Uncharacterized protein experimental SAS data
DAMFILT model
Sample: Uncharacterized protein tetramer, 55 kDa Nitrosospira multiformis protein
Buffer: 5 mM DTT 100 mM NaCl 10 mM Tris-HCl 0.02 % NaN3, pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2010 Feb 12
RgGuinier 2.3 nm
Dmax 7.5 nm
VolumePorod 83 nm3

SASDCC6 – Sensory box domain of the sensory-box/GGDEF protein SO_1695 from Shewanella oneidensis, Northeast Structural Genomics Consortium Target SoR288B

Diguanylate cyclase with PAS sensory domain experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Diguanylate cyclase with PAS sensory domain dimer, 29 kDa Shewanella oneidensis protein
Buffer: 5 mM DTT 100 mM NaCl 10 mM Tris-HCl 0.02 % NaN3, pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2010 Feb 12
RgGuinier 2.0 nm
Dmax 6.4 nm
VolumePorod 48 nm3

SASDCD6 – MucBP domain of the adhesion protein PEPE_0118 from Pediococcus pentosaceus. Northeast Structural Genomics Consortium target id PtR41A

Adhesion exoprotein experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Adhesion exoprotein monomer, 14 kDa Pediococcus pentosaceus protein
Buffer: 5 mM DTT 100 mM NaCl 10 mM Tris-HCl 0.02 % NaN3, pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2010 Feb 12
RgGuinier 2.3 nm
Dmax 8.2 nm
VolumePorod 18 nm3

SASDCE6 – PAS domain of the protein CPS_1291 from Colwellia psychrerythraea. Northeast Structural Genomics Consortium target id CsR222B

Sensory box/GGDEF domain protein experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Sensory box/GGDEF domain protein dimer, 30 kDa Colwellia psychrerythraea protein
Buffer: 5 mM DTT 100 mM NaCl 10 mM Tris-HCl 0.02 % NaN3, pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2010 Feb 12
RgGuinier 2.2 nm
Dmax 7.7 nm
VolumePorod 52 nm3

SASDCF6 – HIT family hydrolase protein from Vibrio fischeri. Northeast Structural Genomics Consortium target id VfR176

HIT family hydrolase experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: HIT family hydrolase dimer, 34 kDa Aliivibrio fischeri protein
Buffer: 5 mM DTT 100 mM NaCl 10 mM Tris-HCl 0.02 % NaN3, pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2010 Feb 12
RgGuinier 2.1 nm
Dmax 7.2 nm
VolumePorod 58 nm3

SASDCG6 – EAL/GGDEF domain protein from M. capsulatus, Northeast Structural Genomics Consortium Target McR174C

EAL/GGDEF domain protein experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: EAL/GGDEF domain protein monomer, 19 kDa Methylococcus capsulatus protein
Buffer: 5 mM DTT 100 mM NaCl 10 mM Tris-HCl 0.02 % NaN3, pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2010 Feb 12
RgGuinier 2.0 nm
Dmax 6.6 nm
VolumePorod 34 nm3

SASDCH6 – GGDEF domain from Marinobacter aquaeolei diguanylate cyclase complexed with c-di-GMP - Northeast Structural Genomics Consortium Target MqR89a

Diguanylate cyclase experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Diguanylate cyclase monomer, 20 kDa Marinobacter hydrocarbonoclasticus protein
Buffer: 5 mM DTT 100 mM NaCl 10 mM Tris-HCl 0.02 % NaN3, pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2010 Feb 12
RgGuinier 1.9 nm
Dmax 6.6 nm
VolumePorod 32 nm3

SASDCJ6 – MmoQ Response regulator (fragment 20-298) from Methylococcus capsulatus str. Bath, Northeast Structural Genomics Consortium Target McR175G

MmoQ experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: MmoQ monomer, 32 kDa Methylococcus capsulatus protein
Buffer: 5 mM DTT 100 mM NaCl 10 mM Tris-HCl 0.02 % NaN3, pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2010 Feb 12
RgGuinier 2.3 nm
Dmax 8.2 nm
VolumePorod 62 nm3

SASDCK6 – Sheath tail protein (DSY3957) from Desulfitobacterium hafniense, Northeast Structural Genomics Consortium Target DhR18

Uncharacterized protein experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Uncharacterized protein monomer, 48 kDa Desulfitobacterium hafniense protein
Buffer: 5 mM DTT 100 mM NaCl 10 mM Tris-HCl 0.02 % NaN3, pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2010 Feb 12
RgGuinier 2.8 nm
Dmax 9.9 nm
VolumePorod 66 nm3