Small Angle Scattering Biological Data Bank SASBDB

SASDCW4 – Bovine β Cardiac Myosin S1 fragment (Myosin-7) MgADP.VO4+OM

Bovine β Cardiac Myosin S1 fragment experimental SAS data

Bovine β Cardiac Myosin S1 fragment Kratky plot

Sample:	Bovine β Cardiac Myosin S1 fragment monomer, 117 kDa Bos taurus protein
Buffer:	10 mM HEPES, 50 mM NaCl, 1 mM NaN3, 2.5 mM MgCl2, 2 mM ADP, 1 mM TCEP, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2016 Mar 1

Mechanistic and structural basis for activation of cardiac myosin force production by omecamtiv mecarbil. Nat Commun 8(1):190 (2017)
Planelles-Herrero VJ, Hartman JJ, Robert-Paganin J, Malik FI, Houdusse A

R_g^Guinier	3.6	nm
D_max	12.6	nm
Volume^Porod	162	nm³

SASDCX4 – Bovine β Cardiac Myosin S1 fragment (Myosin-7) MgADP+OM

Sample:	Bovine β Cardiac Myosin S1 fragment monomer, 117 kDa Bos taurus protein
Buffer:	10 mM HEPES, 50 mM NaCl, 1 mM NaN3, 2.5 mM MgCl2, 2 mM ADP, 1 mM TCEP, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2016 Mar 1

R_g^Guinier	4.0	nm
D_max	11.5	nm
Volume^Porod	161	nm³

SASDCC3 – Nucleolysin TIA-1 isoform p40 in complex with U15 RNA

Nucleolysin TIA-1 isoform p40poly U 15mer experimental SAS data

Nucleolysin TIA-1 isoform p40 poly U 15mer Kratky plot

Sample:	Nucleolysin TIA-1 isoform p40 monomer, 30 kDa Homo sapiens protein poly U 15mer monomer, 5 kDa RNA
Buffer:	10 mM Potassium Phosphate 50 mM NaCl 10 mM DTT, pH: 6
Experiment:	SAXS data collected at Rigaku BioSAXS-1000, SFB 1035, Technische Universität München on 2016 Apr 28

Segmental, Domain-Selective Perdeuteration and Small-Angle Neutron Scattering for Structural Analysis of Multi-Domain Proteins. Angew Chem Int Ed Engl 56(32):9322-9325 (2017)
Sonntag M, Jagtap PKA, Simon B, Appavou MS, Geerlof A, Stehle R, Gabel F, Hennig J, Sattler M

R_g^Guinier	2.4	nm
D_max	8.7	nm
Volume^Porod	39	nm³

SASDEH2 – Unlabeled nucleoporin NUP49/NSP49 (N49) without denaturant

Nucleoporin NUP49/NSP49 experimental SAS data

Unlabeled nucleoporin NUP49/NSP49 (N49) without denaturant Rg histogram

Sample:	Nucleoporin NUP49/NSP49 monomer, 4 kDa Saccharomyces cerevisiae protein
Buffer:	PBS, 10 mM DTT, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Dec 8

Decoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements. Proc Natl Acad Sci U S A 114(31):E6342-E6351 (2017)
Fuertes G, Banterle N, Ruff KM, Chowdhury A, Mercadante D, Koehler C, Kachala M, Estrada Girona G, Milles S, Mishra A, Onck PR, Gräter F, Esteban-Martín S, Pappu RV, Svergun DI, Lemke EA

R_g^Guinier	1.6	nm
D_max	6.0	nm
Volume^Porod	4	nm³

SASDEJ2 – Labeled nucleoporin NUP49/NSP49 (N49-Alexa488/Alexa594) without denaturant

Nucleoporin NUP49/NSP49Alexa Fluor™ 594 C5 MaleimideAlexa Fluor™ 488 C5 Hydroxylamine experimental SAS data

Nucleoporin NUP49/NSP49 Alexa Fluor™ 594 C5 Maleimide Alexa Fluor™ 488 C5 Hydroxylamine Kratky plot

Sample:	Nucleoporin NUP49/NSP49 monomer, 4 kDa Saccharomyces cerevisiae protein Alexa Fluor™ 594 C5 Maleimide monomer, 1 kDa Alexa Fluor™ 488 C5 Hydroxylamine monomer, 1 kDa
Buffer:	PBS, 10 mM DTT, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Dec 8

R_g^Guinier	1.9	nm
D_max	6.5	nm
Volume^Porod	12	nm³

SASDEK2 – Unlabeled nucleoporin NUP49/NSP49 (N49) with denaturant

Unlabeled nucleoporin NUP49/NSP49 (N49) with denaturant Rg histogram

Sample:	Nucleoporin NUP49/NSP49 monomer, 4 kDa Saccharomyces cerevisiae protein
Buffer:	PBS, 10 mM DTT, 6 M urea, 0.3 M KCl, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Dec 8

R_g^Guinier	1.7	nm
D_max	6.5	nm
Volume^Porod	4	nm³

SASDEL2 – Labeled nucleoporin NUP49/NSP49 (N49-Alexa488/Alexa594) with denaturant

Sample:	Nucleoporin NUP49/NSP49 monomer, 4 kDa Saccharomyces cerevisiae protein Alexa Fluor™ 594 C5 Maleimide monomer, 1 kDa Alexa Fluor™ 488 C5 Hydroxylamine monomer, 1 kDa
Buffer:	PBS, 10 mM DTT, 6 M urea, 0.3 M KCl, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Nov 8

R_g^Guinier	2.1	nm
D_max	7.7	nm
Volume^Porod	7	nm³

SASDEM2 – Unlabeled Nuclear Localization Signal (NLS) from the inner nuclear membrane protein HEH2 without denaturant

Inner nuclear membrane protein HEH2 experimental SAS data

Unlabeled Nuclear Localization Signal (NLS) from the inner nuclear membrane protein HEH2 without denaturant Rg histogram

Sample:	Inner nuclear membrane protein HEH2 monomer, 5 kDa Saccharomyces cerevisiae protein
Buffer:	25 mM HEPES, 150 mM NaCl, 10 mM DTT, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2014 Jan 25

R_g^Guinier	2.4	nm
D_max	11.6	nm
Volume^Porod	16	nm³

SASDEN2 – Labeled Nuclear Localization Signal from the inner nuclear membrane protein HEH2 (NLS-Alexa488/Alexa594) without denaturant

Inner nuclear membrane protein HEH2Alexa Fluor™ 594 C5 MaleimideAlexa Fluor™ 488 C5 Hydroxylamine experimental SAS data

Inner nuclear membrane protein HEH2 Alexa Fluor™ 594 C5 Maleimide Alexa Fluor™ 488 C5 Hydroxylamine Kratky plot

Sample:	Inner nuclear membrane protein HEH2 monomer, 5 kDa Saccharomyces cerevisiae protein Alexa Fluor™ 594 C5 Maleimide monomer, 1 kDa Alexa Fluor™ 488 C5 Hydroxylamine monomer, 1 kDa
Buffer:	25 mM HEPES, 150 mM NaCl, 10 mM DTT, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2014 Jan 25

R_g^Guinier	2.0	nm
D_max	7.3	nm
Volume^Porod	10	nm³

SASDEP2 – Unlabeled Nuclear Localization Signal (NLS) from the inner nuclear membrane protein HEH2 with denaturant

Unlabeled Nuclear Localization Signal (NLS) from the inner nuclear membrane protein HEH2 with denaturant Rg histogram

Sample:	Inner nuclear membrane protein HEH2 monomer, 5 kDa Saccharomyces cerevisiae protein
Buffer:	25 mM HEPES, 150 mM NaCl, 10 mM DTT, 6 M urea, 0.3 M KCl, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2014 Jan 25

R_g^Guinier	2.3	nm
D_max	7.1	nm
Volume^Porod	10	nm³

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