SASDTQ4 – Tissue Transglutaminase R580K mutant + 2 mM GTP

Protein-glutamine gamma-glutamyltransferase 2 (R580K) experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 (R580K) Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2 (R580K) , 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17
Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
Aplin C, Zielinski KA, Pabit S, Ogunribido D, Katt WP, Pollack L, Cerione RA, Milano SK
RgGuinier 5.3 nm
Dmax 25.0 nm
VolumePorod 264 nm3

SASDTR4 – Tissue Transglutaminase R580K mutant + 5 mM GTP

Protein-glutamine gamma-glutamyltransferase 2 (R580K) experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 (R580K) Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2 (R580K) , 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17
Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
Aplin C, Zielinski KA, Pabit S, Ogunribido D, Katt WP, Pollack L, Cerione RA, Milano SK
RgGuinier 5.2 nm
Dmax 25.0 nm
VolumePorod 243 nm3

SASDUW3 – Calcium activated full-length gelsolin

Gelsolin experimental SAS data
PYMOL model
Sample: Gelsolin monomer, 84 kDa Homo sapiens protein
Buffer: 25 mM Tris-HCl, pH8, 45 mM NaCl, 1 mM EGTA + 4 mM CaCl2, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Aug 31
Visualizing the nucleating and capped states of f-actin by Ca(2+)-gelsolin: Saxs data based structures of binary and ternary complexes. Int J Biol Macromol :134556 (2024)
Sagar A, Peddada N, Choudhary V, Mir Y, Garg R, Ashish
RgGuinier 4.2 nm
Dmax 16.0 nm
VolumePorod 129 nm3

SASDUX3 – F-actin in F-buffer at an actin concentration of 2 mg/mL

Actin, cytoplasmic 1 experimental SAS data
PYMOL model
Sample: Actin, cytoplasmic 1 18-mer, 751 kDa Gallus gallus protein
Buffer: 50 mM KCl, 50 mM Tris-HCl, pH 8.0, 5 mM MgCl2, 1 mM ATP, 0.1% 2-mercaptoethanol, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Aug 31
Visualizing the nucleating and capped states of f-actin by Ca(2+)-gelsolin: Saxs data based structures of binary and ternary complexes. Int J Biol Macromol :134556 (2024)
Sagar A, Peddada N, Choudhary V, Mir Y, Garg R, Ashish
RgGuinier 13.4 nm
Dmax 48.0 nm

SASDUY3 – Calcium activated full-length gelsolin and the F-form of actin at a 1:32 molar ratio in F-actin buffer

GelsolinActin, cytoplasmic 1 experimental SAS data
Gelsolin Actin, cytoplasmic 1 Kratky plot
Sample: Gelsolin monomer, 84 kDa Homo sapiens protein
Actin, cytoplasmic 1 18-mer, 751 kDa Gallus gallus protein
Buffer: 50 mM KCl, 50 mM Tris-HCl, pH 8.0, 5 mM MgCl2, 1 mM ATP, 0.1% 2-mercaptoethanol, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Aug 31
Visualizing the nucleating and capped states of f-actin by Ca(2+)-gelsolin: Saxs data based structures of binary and ternary complexes. Int J Biol Macromol :134556 (2024)
Sagar A, Peddada N, Choudhary V, Mir Y, Garg R, Ashish
RgGuinier 12.2 nm

SASDUZ3 – Calcium activated full-length gelsolin and the F-form of actin at a 1:16 molar ratio in F-actin buffer

GelsolinActin, cytoplasmic 1 experimental SAS data
Gelsolin Actin, cytoplasmic 1 Kratky plot
Sample: Gelsolin monomer, 84 kDa Homo sapiens protein
Actin, cytoplasmic 1 18-mer, 751 kDa Gallus gallus protein
Buffer: 50 mM KCl, 50 mM Tris-HCl, pH 8.0, 5 mM MgCl2, 1 mM ATP, 0.1% 2-mercaptoethanol, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Aug 31
Visualizing the nucleating and capped states of f-actin by Ca(2+)-gelsolin: Saxs data based structures of binary and ternary complexes. Int J Biol Macromol :134556 (2024)
Sagar A, Peddada N, Choudhary V, Mir Y, Garg R, Ashish
RgGuinier 14.5 nm

SASDU24 – Calcium activated full-length gelsolin and the F-form of actin at a 1:8 molar ratio in F-actin buffer

GelsolinActin, cytoplasmic 1 experimental SAS data
Gelsolin Actin, cytoplasmic 1 Kratky plot
Sample: Gelsolin monomer, 84 kDa Homo sapiens protein
Actin, cytoplasmic 1 18-mer, 751 kDa Gallus gallus protein
Buffer: 50 mM KCl, 50 mM Tris-HCl, pH 8.0, 5 mM MgCl2, 1 mM ATP, 0.1% 2-mercaptoethanol, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Aug 31
Visualizing the nucleating and capped states of f-actin by Ca(2+)-gelsolin: Saxs data based structures of binary and ternary complexes. Int J Biol Macromol :134556 (2024)
Sagar A, Peddada N, Choudhary V, Mir Y, Garg R, Ashish
RgGuinier 13.6 nm

SASDU34 – Calcium activated full-length gelsolin and the F-form of actin at a 1:4 molar ratio in F-actin buffer

GelsolinActin, cytoplasmic 1 experimental SAS data
Gelsolin Actin, cytoplasmic 1 Kratky plot
Sample: Gelsolin monomer, 84 kDa Homo sapiens protein
Actin, cytoplasmic 1 18-mer, 751 kDa Gallus gallus protein
Buffer: 50 mM KCl, 50 mM Tris-HCl, pH 8.0, 5 mM MgCl2, 1 mM ATP, 0.1% 2-mercaptoethanol, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Aug 31
Visualizing the nucleating and capped states of f-actin by Ca(2+)-gelsolin: Saxs data based structures of binary and ternary complexes. Int J Biol Macromol :134556 (2024)
Sagar A, Peddada N, Choudhary V, Mir Y, Garg R, Ashish
RgGuinier 8.8 nm

SASDU44 – Calcium activated full-length gelsolin and the F-form of actin at a 1:2 molar ratio in F-actin buffer

GelsolinActin, cytoplasmic 1 experimental SAS data
Gelsolin Actin, cytoplasmic 1 Kratky plot
Sample: Gelsolin monomer, 84 kDa Homo sapiens protein
Actin, cytoplasmic 1 18-mer, 751 kDa Gallus gallus protein
Buffer: 50 mM KCl, 50 mM Tris-HCl, pH 8.0, 5 mM MgCl2, 1 mM ATP, 0.1% 2-mercaptoethanol, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Aug 31
Visualizing the nucleating and capped states of f-actin by Ca(2+)-gelsolin: Saxs data based structures of binary and ternary complexes. Int J Biol Macromol :134556 (2024)
Sagar A, Peddada N, Choudhary V, Mir Y, Garg R, Ashish
RgGuinier 7.3 nm

SASDU54 – Calcium activated full-length gelsolin and the F-form of actin at a 1:1 molar ratio in F-actin buffer

GelsolinActin, cytoplasmic 1 experimental SAS data
Gelsolin Actin, cytoplasmic 1 Kratky plot
Sample: Gelsolin monomer, 84 kDa Homo sapiens protein
Actin, cytoplasmic 1 18-mer, 751 kDa Gallus gallus protein
Buffer: 50 mM KCl, 50 mM Tris-HCl, pH 8.0, 5 mM MgCl2, 1 mM ATP, 0.1% 2-mercaptoethanol, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Aug 31
Visualizing the nucleating and capped states of f-actin by Ca(2+)-gelsolin: Saxs data based structures of binary and ternary complexes. Int J Biol Macromol :134556 (2024)
Sagar A, Peddada N, Choudhary V, Mir Y, Garg R, Ashish
RgGuinier 5.2 nm

4736 hits found.