| MWexperimental | 98 | kDa |
| MWexpected | 101 | kDa |
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log I(s)
3.86×10-2
3.86×10-3
3.86×10-4
3.86×10-5
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s, nm-1
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Robust structural, kinetic and biophysical characterization of wild-type human ACOD1, selected mutants and their interaction with citraconate
Runge B, Oktay H, Fucci I, Merten E, Tarasov S, Fan L, Monteiro D, Journal of Structural Biology: X :100157 (2026) DOISASD2A2 – Cis-aconitate decarboxylase ACOD1 ACOD1 Arg273Ser mutant
cis-aconitate decarboxylase 1|
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Fits and models
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Synchrotron SAXS
data from solutions of
Cis-aconitate decarboxylase ACOD1 ACOD1 Arg273Ser mutant
in
20 mM HEPES , 150 mM NaCl, 0.5 mM TCEP, and 2% (v/v) glycerol, pH 7.5
were collected
on the
12-ID-B beam line
at the Advanced Photon Source (APS), Argonne National Laboratory storage ring
(Lemont, IL, USA)
using a Eiger2 S detector
at a sample-detector distance of 2 m and
at a wavelength of λ = 0.0932 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 100.00 μl sample
at 6.1 mg/ml was injected at a 0.30 ml/min flow rate
onto a GE Superdex 200 Increase 5/150 column
at 20°C.
694 successive
0.300 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
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