Robust structural, kinetic and biophysical characterization of wild-type human ACOD1, selected mutants and their interaction with citraconate

Runge B, Oktay H, Fucci I, Merten E, Tarasov S, Fan L, Monteiro D
Journal of Structural Biology: X :100157 (2026 Aug)

doi: 10.1016/j.yjsbx.2026.100157 		Submitted to SASBDB: 2026 Jun 24
Published in SASBDB:

SASD272 – Wild-type Cis-aconitate decarboxylase (ACOD1)

cis-aconitate decarboxylase 1 experimental SAS data
OTHER model
Sample: Cis-aconitate decarboxylase 1 dimer, 101 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 0.5 mM TCEP, 2% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at 12-ID-B, Advanced Photon Source (APS), Argonne National Laboratory on 2026 Mar 6
RgGuinier 3.2 nm

SASD282 – Citraconate-bound Cis-aconitate decarboxylase (ACOD1)

cis-aconitate decarboxylase 1 experimental SAS data
OTHER model
Sample: Cis-aconitate decarboxylase 1 dimer, 101 kDa Homo sapiens protein
Buffer: 20 mM HEPES , 150 mM NaCl, 0.5 mM TCEP, and 2% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at 12-ID-B, Advanced Photon Source (APS), Argonne National Laboratory on 2026 Mar 6
RgGuinier 3.2 nm

SASD2A2 – Cis-aconitate decarboxylase ACOD1 ACOD1 Arg273Ser mutant

cis-aconitate decarboxylase 1 experimental SAS data
OTHER model
Sample: Cis-aconitate decarboxylase 1 dimer, 101 kDa Homo sapiens protein
Buffer: 20 mM HEPES , 150 mM NaCl, 0.5 mM TCEP, and 2% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at 12-ID-B, Advanced Photon Source (APS), Argonne National Laboratory on 2026 Mar 6
RgGuinier 3.1 nm

SASD2B2 – Cis-aconitate decarboxylase ACOD1 Tyr318Ala mutant

cis-aconitate decarboxylase 1 experimental SAS data
OTHER model
Sample: Cis-aconitate decarboxylase 1 dimer, 101 kDa Homo sapiens protein
Buffer: 20 mM HEPES , 150 mM NaCl, 0.5 mM TCEP, and 2% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at 12-ID-B, Advanced Photon Source (APS), Argonne National Laboratory on 2026 Mar 6
RgGuinier 3.1 nm

SASD292 – Cis-aconitate decarboxylase ACOD1 His103Ala mutant

cis-aconitate decarboxylase 1 experimental SAS data
OTHER model
Sample: Cis-aconitate decarboxylase 1 dimer, 101 kDa Homo sapiens protein
Buffer: 20 mM HEPES , 150 mM NaCl, 0.5 mM TCEP, and 2% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at 12-ID-B, Advanced Photon Source (APS), Argonne National Laboratory on 2026 Mar 6
RgGuinier 3.1 nm