Beta-Heavy-Spectrin segments 25 to 31 homodimerization

Chelsea Sarring.

SASD2D2 – Beta-Heavy-Spectrin fragment containing segments 25 to 31

Beta-Heavy-Spectrin
MWexperimental 168 kDa
MWexpected 173 kDa
VPorod 453 nm3
log I(s) 2.47×100 2.47×10-1 2.47×10-2 2.47×10-3
Beta-Heavy-Spectrin small angle scattering data  s, nm-1
ln I(s)
Beta-Heavy-Spectrin Guinier plot ln 2.47×100 Rg: 7.8 nm 0 (7.8 nm)-2 s2
(sRg)2I(s)/I(0)
Beta-Heavy-Spectrin Kratky plot 1.104 0 √3 sRg
p(r)
Beta-Heavy-Spectrin pair distance distribution function Rg: 10.3 nm 0 Dmax: 35.8 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Beta-Heavy-Spectrin CORAL model
SAXS data from solutions of Beta-Heavy-Spectrin fragment containing segments 25 to 31 in 10 mM NaH2PO4, 130 mM NaCl, pH 7.4 were collected on the Rigaku BioSAXS-2000 instrument (Pennsylvania State University, University Park, PA, USA) using a Rigaku HyPix-3000 detector at a sample-detector distance of 0.5 m and at a wavelength of λ = 0.154 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 0.55 mg/ml was measured at 4°C. Six successive 600 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

The entry contains a dataset of the same sample at a concentration of 1.7 mg/ml, which is available for download as an additional file.

Beta-Heavy-Spectrin (Beta[H])
Mol. type   Protein
Organism   Drosophila melanogaster
Olig. state   Dimer
Mon. MW   86.6 kDa
 
UniProt   Q9VZQ3 (2773-3521)
Sequence   FASTA