Solution structures of glycosaminoglycan-bound CXCL8 complexes determined by small-angle X-ray scattering (SAXS).

White MA Mahler BP, Joseph PRB, Nagarajan B, Wang X, Desai UR, Rajarathnam K, Biochem J 483(10):1861-1876 (2026) Europe PMC

SASD2N2 – Heparin 8-mer

Heparin 8-mer
MWexperimental 2 kDa
MWexpected 2 kDa
log I(s) 1.39×104 1.39×103 1.39×102 1.39×101
Heparin 8-mer small angle scattering data  s, nm-1
ln I(s)
Heparin 8-mer Guinier plot ln 1.39×104 Rg: 1.0 nm 0 (1.0 nm)-2 s2
(sRg)2I(s)/I(0)
Heparin 8-mer Kratky plot 1.104 0 √3 sRg
p(r)
Heparin 8-mer pair distance distribution function Rg: 1.0 nm 0 Dmax: 3.3 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Heparin 8-mer OTHER model
Heparin 8-mer OTHER model
Heparin 8-mer OTHER model
Heparin 8-mer OTHER model
SAXS data from solutions of Heparin 8-mer in 20 mM sodium succinate, 100 mM NaCl, 5 mM MgCl2, pH 5.5 were collected on the Rigaku BioSAXS-1000 instrument (Sealy Center For Structural Biology, UTMB-G, Galveston, TX, USA) using a Pilatus 100K detector at a sample-detector distance of 0.5 m and at a wavelength of λ = 0.15418 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 5.00 mg/ml was measured at 5°C. 12 successive 3600 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Heparin 8-mer (Hep08)
Mol. type   Other
Olig. state   Monomer
Mon. MW   2.4 kDa
PDB ID   1HEP
 
PDB ID   1HEP
 
PDB ID   1HEP
 
PDB ID   1HEP