Solution structures of glycosaminoglycan-bound CXCL8 complexes determined by small-angle X-ray scattering (SAXS).

White MA Mahler BP, Joseph PRB, Nagarajan B, Wang X, Desai UR, Rajarathnam K, Biochem J 483(10):1861-1876 (2026) Europe PMC

SASD2P2 – Chondroitin 8-mer

Chondroitin sulfate 8-mer

MW^I(0)	2	kDa
MW^expected	2	kDa

log I(s) 1.85×10³ 1.85×10² 1.85×10¹ 1.85×10⁰

Chondroitin sulfate 8-mer small angle scattering data

s, nm^-1

Log plot Log-Log plot

ln I(s)

ln 1.86×10³ R_g: 1.0 nm 0 (1.0 nm)^-2 s²

(sR_g)²I(s)/I(0)

1.104 0 √3 sR_g

p(r)	R_g: 1.1 nm 0 D_max: 3.7 nm

Data validation

Experimental data range

p(r) fit to data

Metric

Value

s_min D_max / π

0.4

N_Shannon

Worse

Better

Metric

Value

p_cormap

χ²

0.007
0.783

Worse

Better

Fits and models

log I(s)	s, nm^-1
+3 Δ/σ -3	s, nm^-1

Chondroitin sulfate 8-mer CUSTOM IN-HOUSE model

SAXS data from solutions of Chondroitin 8-mer in Phosphate buffer, pH 7 were collected on the Rigaku BioSAXS-1000 instrument (Sealy Center For Structural Biology, UTMB-G, Galveston, TX, USA) using a Pilatus 100K detector at a sample-detector distance of 0.5 m and at a wavelength of λ = 0.15418 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 10.00 mg/ml was measured at 5°C. 10 successive 3600 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Chondroitin sulfate 8-mer (CS08)
Mol. type		Other
Organism		NA
Olig. state		Monomer
Mon. MW		1.8 kDa
Chemical formula

PDB ID		3IL8