Solution structures of glycosaminoglycan-bound CXCL8 complexes determined by small-angle X-ray scattering (SAXS).

White MA Mahler BP, Joseph PRB, Nagarajan B, Wang X, Desai UR, Rajarathnam K, Biochem J 483(10):1861-1876 (2026) Europe PMC

SASD2Q2 – Interleukin-8 (CXCL8)

INTERLEUKIN 8
MWexperimental 12 kDa
MWexpected 16 kDa
log I(s) 2.50×104 2.50×103 2.50×102 2.50×101
INTERLEUKIN 8 small angle scattering data  s, nm-1
ln I(s)
INTERLEUKIN 8 Guinier plot ln 2.51×104 Rg: 1.6 nm 0 (1.6 nm)-2 s2
(sRg)2I(s)/I(0)
INTERLEUKIN 8 Kratky plot 1.104 0 √3 sRg
p(r)
INTERLEUKIN 8 pair distance distribution function Rg: 1.6 nm 0 Dmax: 5.2 nm

Data validation

Fits and models

log I(s)
 s, nm-1
INTERLEUKIN 8 PDB (PROTEIN DATA BANK) model
SAXS data from solutions of Interleukin-8 (CXCL8) in Phosphate buffer, pH 7 were collected on the Rigaku BioSAXS-1000 instrument (Sealy Center For Structural Biology, UTMB-G, Galveston, TX, USA) using a Pilatus 100K detector at a sample-detector distance of 0.5 m and at a wavelength of λ = 0.15418 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 4.00 mg/ml was measured at 5°C. Eight successive 3600 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

INTERLEUKIN 8 (CXCL8)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Dimer
Mon. MW   8.0 kDa
 
UniProt   P10145 (28-99)
Sequence   FASTA
 
PDB ID   3IL8