| MWI(0) | 28 | kDa |
| MWexpected | 18 | kDa |
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log I(s)
2.76×104
2.76×103
2.76×102
2.76×101
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s, nm-1
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Solution structures of glycosaminoglycan-bound CXCL8 complexes determined by small-angle X-ray scattering (SAXS).
White MA Mahler BP, Joseph PRB, Nagarajan B, Wang X, Desai UR, Rajarathnam K, Biochem J 483(10):1861-1876 (2026) Europe PMCSASD2R2 – Interleukin-8-Chondroitin sulphate (CXCL8:CS08)
Chondroitin sulfate 8-merINTERLEUKIN 8
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Fits and models
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SAXS
data from solutions of
Interleukin-8-Chondroitin sulphate (CXCL8:CS08)
in
50 mM phosphate, pH 7
were collected
on the
Rigaku BioSAXS-1000 instrument (Sealy Center For Structural Biology, UTMB-G, Galveston, TX, USA)
using a Pilatus 100K detector
at a sample-detector distance of 0.5 m and
at a wavelength of λ = 0.15418 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
One solute concentration of 10.00 mg/ml was measured
at 5°C.
Eight successive
3600 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
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