Standard proteins

Darja Ruskule.

SASDA32 – Bovine serum albumin in HEPES

Bovine Serum Albumin
MWI(0) 64 kDa
MWexpected 69 kDa
VPorod 97 nm3
log I(s) 5.93×101 5.93×100 5.93×10-1 5.93×10-2
Bovine Serum Albumin small angle scattering data  s, nm-1
ln I(s)
Bovine Serum Albumin Guinier plot ln 5.93×101 Rg: 2.9 nm 0 (2.9 nm)-2 s2
Bovine Serum Albumin Kratky plot 1.104 0 3 sRg
Bovine Serum Albumin pair distance distribution function Rg: 2.8 nm 0 Dmax: 9.3 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Bovine Serum Albumin DAMMIF model

log I(s)
 s, nm-1
Bovine Serum Albumin NONE model

Synchrotron SAXS data from solutions of Bovine serum albumin in HEPES in 50 mM HEPES 50 mM KCl, pH 7.5 were collected on the EMBL X33 beam line at the DORIS III, DESY storage ring (Hamburg, Germany) using a Pilatus 1M-W detector at a sample-detector distance of 2.7 m and at a wavelength of λ = 0.15 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 25.64 mg/ml was measured at 10°C. Eight successive 15 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Tags: X33
Bovine Serum Albumin (BSA)
Mol. type   Protein
Organism   Bos taurus (Bovine)
Olig. state   Monomer
Mon. MW   69.4 kDa
Sequence   FASTA
PDB ID   3V03