Standard proteins

Darja Ruskule Published in SASBDB:

SASDA32 – BSA in HEPES

Serum albumin experimental SAS data
DAMMIF model
Sample: Serum albumin monomer, 69 kDa Bos taurus protein
Buffer: 50 mM HEPES 50 mM KCl, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III on 2012 Sep 12
RgGuinier 2.9 nm
Dmax 9.3 nm
VolumePorod 97 nm3

SASDA52 – Alcohol dehydrogenase in PBS

Alcohol dehydrogenase 1 experimental SAS data
PDB model
Sample: Alcohol dehydrogenase 1 tetramer, 147 kDa Saccharomyces cerevisiae protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at EMBL X33, DORIS III on 2012 Sep 20
RgGuinier 3.2 nm
Dmax 8.9 nm
VolumePorod 178 nm3

SASDA62 – bAmylase in PBS

Beta-amylase experimental SAS data
DAMMIF model
Sample: Beta-amylase tetramer, 224 kDa Ipomoea batatas protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at EMBL X33, DORIS III on 2012 Sep 20
RgGuinier 4.2 nm
Dmax 12.7 nm
VolumePorod 214 nm3

SASDA82 – Apoferritin in PBS and glycerol

Ferritin light chain experimental SAS data
PDB model
Sample: Ferritin light chain 24-mer, 479 kDa Equus caballus protein
Buffer: PBS, 50% Glycerol, 0.076 M NaCl, pH: 7.4
Experiment: SAXS data collected at EMBL X33, DORIS III on 2012 Sep 20
RgGuinier 6.8 nm
Dmax 12.8 nm
VolumePorod 619 nm3

SASDA92 – Catalase in PBS

Catalase experimental SAS data
PDB model
Sample: Catalase tetramer, 240 kDa Bos taurus protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at EMBL X33, DORIS III on 2012 Sep 17
RgGuinier 4.1 nm
Dmax 10.9 nm
VolumePorod 296 nm3

SASDAA2 – Conalbumin in PBS

Ovotransferrin experimental SAS data
DAMMIF model
Sample: Ovotransferrin monomer, 78 kDa Gallus gallus protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at EMBL X33, DORIS III on 2012 Sep 21
RgGuinier 3.8 nm
Dmax 11.0 nm
VolumePorod 117 nm3

SASDAB2 – Cytochrome c from equine heart

Cytochrome cHeme C experimental SAS data
PDB model
Sample: Cytochrome c monomer, 12 kDa Equus caballus protein
Heme C monomer, 1 kDa
Buffer: 25 mM HEPES, 100 mM NaCl, 3% v/v glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Apr 8
RgGuinier 1.3 nm
Dmax 3.7 nm
VolumePorod 12 nm3

SASDAC2 – Lyz in Sodium Acetate

Lysozyme C experimental SAS data
CRYSOL model
Sample: Lysozyme C monomer, 14 kDa Gallus gallus protein
Buffer: 40 mM Sodium Acetate, pH: 3.8
Experiment: SAXS data collected at EMBL X33, DORIS III on 2012 Sep 21
RgGuinier 1.5 nm
Dmax 4.0 nm
VolumePorod 17 nm3

SASDAG2 – Lyz in Sodium Acetate

Lysozyme C experimental SAS data
GASBOR model
Sample: Lysozyme C monomer, 14 kDa Gallus gallus protein
Buffer: 40 mM Sodium Acetate, pH: 3.8
Experiment: WAXS data collected at EMBL X33, DORIS III on 2012 Sep 21
RgGuinier 1.5 nm
Dmax 4.0 nm
VolumePorod 21 nm3

SASDAH2 – Myo in PBS

Myoglobin experimental SAS data
CRYSOL model
Sample: Myoglobin monomer, 17 kDa Equus caballus protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at EMBL X33, DORIS III on 2012 Sep 20
RgGuinier 1.7 nm
Dmax 5.0 nm
VolumePorod 32 nm3

SASDAK2 – Myo in PBS

Myoglobin experimental SAS data
PDB model
Sample: Myoglobin monomer, 17 kDa Equus caballus protein
Buffer: PBS, pH: 7.4
Experiment: WAXS data collected at EMBL X33, DORIS III on 2012 Sep 20
RgGuinier 1.6 nm
Dmax 5.0 nm
VolumePorod 32 nm3

SASDAL2 – Ovalbumin in PBS

Ovalbumin experimental SAS data
DAMMIN model
Sample: Ovalbumin dimer, 86 kDa Gallus gallus protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at EMBL X33, DORIS III on 2012 Sep 18
RgGuinier 2.5 nm
Dmax 7.8 nm
VolumePorod 74 nm3

SASDAN2 – RNase in PBS

Ribonuclease pancreatic experimental SAS data
GASBOR model
Sample: Ribonuclease pancreatic monomer, 16 kDa Bos taurus protein
Buffer: PBS, pH: 7.4
Experiment: WAXS data collected at EMBL X33, DORIS III on 2012 Sep 18
RgGuinier 1.6 nm
Dmax 5.0 nm
VolumePorod 15 nm3

SASDAQ2 – Ubiquitin in sodium acetate

Ubiquitin experimental SAS data
PDB model
Sample: Ubiquitin monomer, 9 kDa Bos taurus protein
Buffer: 40 mM Sodium acetate 150 mM NaCl, pH: 5.5
Experiment: SAXS data collected at EMBL X33, DORIS III on 2012 Sep 18
RgGuinier 1.3 nm
Dmax 4.9 nm
VolumePorod 12 nm3

SASDAR2 – RNase in PBS

Ribonuclease pancreatic experimental SAS data
DAMMIF model
Sample: Ribonuclease pancreatic monomer, 16 kDa Bos taurus protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at EMBL X33, DORIS III on 2012 Sep 18
RgGuinier 1.6 nm
Dmax 5.0 nm
VolumePorod 17 nm3

SASDAK6 – Glucose Isomerase

Xylose isomerase experimental SAS data
DAMMIF model
Sample: Xylose isomerase tetramer, 172 kDa Streptomyces rubiginosus protein
Buffer: PBS, 50% Glycerol, 0.076 M NaCl, pH: 7.4
Experiment: SAXS data collected at EMBL X33, DORIS III on 2012 Apr 24
RgGuinier 3.4 nm
Dmax 9.7 nm
VolumePorod 293 nm3