Standard proteins

Darja Ruskule.

SASDA52 – Alcohol dehydrogenase in PBS

Alcohol dehydrogenase 1
MWI(0) 79 kDa
MWexpected 147 kDa
VPorod 178 nm3
log I(s) 7.65×101 7.65×100 7.65×10-1 7.65×10-2
Alcohol dehydrogenase 1 small angle scattering data  s, nm-1
ln I(s)
Alcohol dehydrogenase 1 Guinier plot ln 7.66×101 Rg: 3.2 nm 0 (3.2 nm)-2 s2
Alcohol dehydrogenase 1 Kratky plot 1.104 0 3 sRg
Alcohol dehydrogenase 1 pair distance distribution function Rg: 3.2 nm 0 Dmax: 8.9 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Alcohol dehydrogenase 1 PDB (PROTEIN DATA BANK) model

log I(s)
 s, nm-1
Alcohol dehydrogenase 1 DAMMIF model

Synchrotron SAXS data from solutions of Alcohol dehydrogenase in PBS in PBS, pH 7.4 were collected on the EMBL X33 beam line at the DORIS III storage ring (Hamburg, Germany) using a Pilatus 1M-W detector at a sample-detector distance of 2.7 m and at a wavelength of λ = 0.15 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 24.89 mg/ml was measured at 10°C. Eight successive 15 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Tags: X33
Alcohol dehydrogenase 1 (adh)
Mol. type   Protein
Organism   Saccharomyces cerevisiae
Olig. state   Tetramer
Mon. MW   36.9 kDa
UniProt   P00330
Sequence   FASTA