Standard proteins

Darja Ruskule.

SASDA82 – Apoferritin in PBS and glycerol

Ferritin light chain
MWI(0) 369 kDa
MWexpected 479 kDa
VPorod 619 nm3
log I(s) 3.56×102 3.56×101 3.56×100 3.56×10-1
Ferritin light chain small angle scattering data  s, nm-1
ln I(s)
Ferritin light chain Guinier plot ln 3.56×102 Rg: 6.8 nm 0 (6.8 nm)-2 s2
(sRg)2I(s)/I(0)
Ferritin light chain Kratky plot 1.104 0 √3 sRg
p(r)
Ferritin light chain pair distance distribution function Rg: 5.2 nm 0 Dmax: 12.8 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Ferritin light chain PDB (PROTEIN DATA BANK) model
log I(s)
 s, nm-1
Ferritin light chain DAMMIF model
Synchrotron SAXS data from solutions of Apoferritin in PBS and glycerol in PBS, 50% Glycerol, 0.076 M NaCl, pH 7.4 were collected on the EMBL X33 beam line at the DORIS III, DESY storage ring (Hamburg, Germany) using a Pilatus 1M-W detector at a sample-detector distance of 2.7 m and at a wavelength of λ = 0.15 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 18.53 mg/ml was measured at 10°C. Eight successive 15 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Tags: X33
Ferritin light chain (Apoferritin)
Mol. type   Protein
Organism   Equus caballus
Olig. state   24-mer
Mon. MW   20.0 kDa
 
UniProt   P02791
Sequence   FASTA
 
PDB ID   1IER