The crystal structure of netrin-1 in complex with DCC reveals the bifunctionality of netrin-1 as a guidance cue.

Finci LI, Kr├╝ger N, Sun X, Zhang J, Chegkazi M, Wu Y, Schenk G, Mertens HDT, Svergun DI, Zhang Y, Wang JH, Meijers R, Neuron 83(4):839-849 (2014) Europe PMC

SASDA86 – NetrinVIV DCC56(M933R) complex

Netrin-1
Deleted in Colorectal Cancer (FN5 & FN6) M933R mutant
MWexperimental 42 kDa
MWexpected 75 kDa
VPorod 95 nm3
log I(s) 1.27×103 1.27×102 1.27×101 1.27×100
Netrin-1 Deleted in Colorectal Cancer (FN5 & FN6) M933R mutant small angle scattering data  s, nm-1
ln I(s)
Netrin-1 Deleted in Colorectal Cancer (FN5 & FN6) M933R mutant Guinier plot ln 1.27×103 Rg: 4 nm 0 (4 nm)-2 s2
(sRg)2I(s)/I(0)
Netrin-1 Deleted in Colorectal Cancer (FN5 & FN6) M933R mutant Kratky plot 1.104 0 3 sRg
p(r)
Netrin-1 Deleted in Colorectal Cancer (FN5 & FN6) M933R mutant pair distance distribution function Rg: 4 nm 0 Dmax: 14 nm

Data validation


There are no models related to this curve.

Synchrotron SAXS data from solutions of NetrinVIV DCC56(M933R) complex in 25 MES mM 200 mM NaCl 50 mM Tris 0.2 M ammonium sulfate (NH4)2(SO4) 1mM calcium chloride CaCl2, pH 7 were collected on the EMBL P12 beam line at the PETRA III storage ring (Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3.1 m and at a wavelength of λ = 0.12 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 0.3 and 3.9 mg/ml were measured at 10°C. 20 successive 0.050 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Netrin-1 (NetrinVIV)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   49.1 kDa
 
UniProt   O95631
Sequence   FASTA
 
Deleted in Colorectal Cancer (FN5 & FN6) M933R mutant (DCC56 (M933R))
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   25.5 kDa
Sequence   FASTA