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58 hits found for Mertens

SASDAZ5 – NetrinVIV

Netrin-1 experimental SAS data
DAMMIN model
Sample: Netrin-1 monomer, Homo sapiens protein
Buffer: 25 MES mM 200 mM NaCl 50 mM Tris 0.2 M ammonium sulfate (NH4)2(SO4) 1mM calcium chloride CaCl2, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Aug 26
The crystal structure of netrin-1 in complex with DCC reveals the bifunctionality of netrin-1 as a guidance cue. Neuron 83(4):839-849 (2014)
...Mertens HDT, Svergun DI, Zhang Y, Wang JH, Meijers R
RgGuinier 3.9 nm
Dmax 13.5 nm
VolumePorod 100 nm3

SASDA26 – DCC56

Deleted in Colorectal Cancer (FN5 & FN6) experimental SAS data
DAMMIN model
Sample: Deleted in Colorectal Cancer (FN5 & FN6) monomer, Homo sapiens protein
Buffer: 25 MES mM 200 mM NaCl 50 mM Tris 0.2 M ammonium sulfate (NH4)2(SO4) 1mM calcium chloride CaCl2, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Aug 26
The crystal structure of netrin-1 in complex with DCC reveals the bifunctionality of netrin-1 as a guidance cue. Neuron 83(4):839-849 (2014)
...Mertens HDT, Svergun DI, Zhang Y, Wang JH, Meijers R
RgGuinier 3.2 nm
Dmax 11.0 nm
VolumePorod 38 nm3

SASDC32 – Ethylene Receptor 1 Cytosolic Domain (in 250 mM NDSB)

Ethylene Receptor 1 experimental SAS data
Ethylene Receptor 1 Cytosolic Domain (in 250 mM NDSB) Rg histogram
Sample: Ethylene Receptor 1 dimer, Arabidopsis thaliana protein
Buffer: 20 mM Tris-NDSB 150 mM NaCl 1mM DTT 250 mM NDSB, pH: 8.8
Experiment: SAXS data collected at EMBL X33, DORIS III on 2011 Mar 19
Structural model of the cytosolic domain of the plant ethylene receptor 1 (ETR1). J Biol Chem 290(5):2644-58 (2015)
...Mertens HD, Mueller-Dieckmann J
RgGuinier 4.7 nm
Dmax 15.8 nm
VolumePorod 316 nm3

SASDC42 – Ethylene Receptor 1 Cytosolic Domain

Ethylene Receptor 1 experimental SAS data
Ethylene Receptor 1 Cytosolic Domain Rg histogram
Sample: Ethylene Receptor 1 dimer, Arabidopsis thaliana protein
Buffer: 20 mM TRIS 150 mM NaCl 1mM DTT 250mM NDSB, pH: 8.8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Mar 29
Structural model of the cytosolic domain of the plant ethylene receptor 1 (ETR1). J Biol Chem 290(5):2644-58 (2015)
...Mertens HD, Mueller-Dieckmann J
RgGuinier 5.5 nm
Dmax 19.0 nm
VolumePorod 274 nm3

SASDB52 – Death associated protein kinase (wild-type)

Death associated protein kinase wild-type experimental SAS data
NONE model
Sample: Death associated protein kinase wild-type other, Homo sapiens protein
Buffer: 50 mM HEPES 250 mM NaCl 5mM CaCl2 0.25 mM TCEP 5% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Dec 18
Death-Associated Protein Kinase Activity Is Regulated by Coupled Calcium/Calmodulin Binding to Two Distinct Sites. Structure 24(6):851-61 (2016)
...Mertens HD, Komadina D, Hoffmann JE, Yumerefendi H, Svergun DI, Kursula P, Schultz C, McCarthy AA, Hart DJ, Wilmanns M
RgGuinier 2.7 nm
Dmax 8.1 nm
VolumePorod 101 nm3

SASDB62 – Death associated protein kinase (D220K mutant)

Death associated protein kinase (D220K mutant) experimental SAS data
NONE model
Sample: Death associated protein kinase (D220K mutant) other, Homo sapiens protein
Buffer: 50 mM HEPES 250 mM NaCl 5mM CaCl2 0.25 mM TCEP 5% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Dec 18
Death-Associated Protein Kinase Activity Is Regulated by Coupled Calcium/Calmodulin Binding to Two Distinct Sites. Structure 24(6):851-61 (2016)
...Mertens HD, Komadina D, Hoffmann JE, Yumerefendi H, Svergun DI, Kursula P, Schultz C, McCarthy AA, Hart DJ, Wilmanns M
RgGuinier 2.5 nm
Dmax 9.0 nm
VolumePorod 89 nm3

SASDA76 – NetrinVIV DCC56 complex

Netrin-1Deleted in Colorectal Cancer (FN5 & FN6) experimental SAS data
Netrin-1 Deleted in Colorectal Cancer (FN5 & FN6) Kratky plot
Sample: Netrin-1 monomer, Homo sapiens protein
Deleted in Colorectal Cancer (FN5 & FN6) monomer, Homo sapiens protein
Buffer: 25 MES mM 200 mM NaCl 50 mM Tris 0.2 M ammonium sulfate (NH4)2(SO4) 1mM calcium chloride CaCl2, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Oct 31
The crystal structure of netrin-1 in complex with DCC reveals the bifunctionality of netrin-1 as a guidance cue. Neuron 83(4):839-849 (2014)
...Mertens HDT, Svergun DI, Zhang Y, Wang JH, Meijers R
RgGuinier 5.1 nm
Dmax 17.0 nm
VolumePorod 120 nm3

SASDA86 – NetrinVIV DCC56(M933R) complex

Netrin-1Deleted in Colorectal Cancer (FN5 & FN6) M933R mutant experimental SAS data
Netrin-1 Deleted in Colorectal Cancer (FN5 & FN6) M933R mutant Kratky plot
Sample: Netrin-1 monomer, Homo sapiens protein
Deleted in Colorectal Cancer (FN5 & FN6) M933R mutant monomer, Homo sapiens protein
Buffer: 25 MES mM 200 mM NaCl 50 mM Tris 0.2 M ammonium sulfate (NH4)2(SO4) 1mM calcium chloride CaCl2, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Aug 26
The crystal structure of netrin-1 in complex with DCC reveals the bifunctionality of netrin-1 as a guidance cue. Neuron 83(4):839-849 (2014)
...Mertens HDT, Svergun DI, Zhang Y, Wang JH, Meijers R
RgGuinier 4.0 nm
Dmax 14.0 nm
VolumePorod 95 nm3

SASDBE7 – ESX-5 type VII secretion system protein EccC5

ESX-5 type VII secretion system protein EccC5 experimental SAS data
ESX-5 type VII secretion system protein EccC5 Rg histogram
Sample: ESX-5 type VII secretion system protein EccC5 monomer, Mycobacterium xenopi RIVM700367 protein
Buffer: 20 mM Tris 200 mM NaCl 5%(v/v) Glycerol, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Jul 15
Structure of the mycobacterial ESX-5 type VII secretion system membrane complex by single-particle analysis. Nat Microbiol 2:17047 (2017)
...Mertens HD, Ummels R, Lugmayr W, Mayr J, Rettel M, Savitski MM, Svergun DI, Bitter W, Wilmanns M, Marlovits TC, Parret AH, Houben EN
RgGuinier 6.2 nm
Dmax 23.0 nm
VolumePorod 255 nm3

SASDDE5 – NBD-MsbA (apo)

Nucleotide Binding Domain of Lipid A export ATP-binding/permease protein MsbA experimental SAS data
Nucleotide Binding Domain of Lipid A export ATP-binding/permease protein MsbA Kratky plot
Sample: Nucleotide Binding Domain of Lipid A export ATP-binding/permease protein MsbA monomer, Escherichia coli (strain ... protein
Buffer: 30 mM Tris, 150 mM NaCl, 0.5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 May 30
Conformational States of ABC Transporter MsbA in a Lipid Environment Investigated by Small-Angle Scattering Using Stealth Carrier Nanodiscs. Structure 26(8):1072-1079.e4 (2018)
...Mertens HD, Moulin M, Haertlein M, Prevost S, Svergun DI, Busch S, Forsyth VT, Tidow H
RgGuinier 2.2 nm
Dmax 7.3 nm
VolumePorod 47 nm3

SASDDF5 – NBD-MsbA (+1mM ADP)

Nucleotide Binding Domain of Lipid A export ATP-binding/permease protein MsbA experimental SAS data
Nucleotide Binding Domain of Lipid A export ATP-binding/permease protein MsbA Kratky plot
Sample: Nucleotide Binding Domain of Lipid A export ATP-binding/permease protein MsbA monomer, Escherichia coli (strain ... protein
Buffer: 30 mM Tris, 150 mM NaCl, 0.5 mM TCEP, 1 mM ADP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 May 30
Conformational States of ABC Transporter MsbA in a Lipid Environment Investigated by Small-Angle Scattering Using Stealth Carrier Nanodiscs. Structure 26(8):1072-1079.e4 (2018)
...Mertens HD, Moulin M, Haertlein M, Prevost S, Svergun DI, Busch S, Forsyth VT, Tidow H
RgGuinier 2.1 nm
Dmax 7.3 nm
VolumePorod 50 nm3

SASDET4 – ACA8 complex with Calmodulin (75% deuterated) in stealth nanodisc (SANS, 100% D2O)

Membrane scaffold protein 1D1 (deuterated, 75%)1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl)Calcium-transporting ATPase 8, plasma membrane-typeCalmodulin-7 (deuterated 75%) experimental SAS data
ACA8 complex with Calmodulin (75% deuterated) in stealth nanodisc (SANS, 100% D2O) Rg histogram
Sample: Membrane scaffold protein 1D1 (deuterated, 75%) dimer, None protein
1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl) unknown, Escherichia coli. (AL95 ... other
Calcium-transporting ATPase 8, plasma membrane-type monomer, Arabidopsis thaliana protein
Calmodulin-7 (deuterated 75%) monomer, Arabidopsis thaliana protein
Buffer: 30 mM Tris, 150 mM NaCl, 1mM MgCl2, 1 mM CaCl2, pH: 7.5
Experiment: SANS data collected at SANS-1, Heinz Maier-Leibnitz Zentrum on 2017 Aug 28
Structural basis for activation of plasma-membrane Ca2+-ATPase by calmodulin Communications Biology 1(1) (2018)
...Mertens H, Maric S, Moulin M, Haertlein M, Busch S, Forsyth V, Svergun D, Uetrecht C, Tidow H
RgGuinier 4.3 nm
Dmax 15.0 nm
VolumePorod 217 nm3

SASDCX6 – T2 SapNP (SapA:POPS) (SEC-SAXS)

saposin-a1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serineMechanosensitive channel T2 experimental SAS data
DAMMIF model
Sample: saposin-a monomer, Homo sapiens protein
1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serine other, Synthetic compound other
Mechanosensitive channel T2 heptamer, Thermoplasma volcanium (strain ... protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 May 4
Saposin Lipid Nanoparticles: A Highly Versatile and Modular Tool for Membrane Protein Research. Structure 26(2):345-355.e5 (2018)
...Mertens HDT, Ural-Blimke Y, Martinez Molledo M, Svergun DI, Löw C
RgGuinier 5.4 nm
Dmax 18.0 nm
VolumePorod 686 nm3

SASDCY6 – T2 in b-DDM micelle (SEC-SAXS)

Mechanosensitive channel T2n-Dodecyl-β-D-Maltopyranoside experimental SAS data
DAMMIF model
Sample: Mechanosensitive channel T2 heptamer, Thermoplasma volcanium (strain ... protein
n-Dodecyl-β-D-Maltopyranoside other, Synthetic compound other
Buffer: 20 mM Tris 150 mM NaCl 5 % glycerol 0.03 % DDM, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 May 29
Saposin Lipid Nanoparticles: A Highly Versatile and Modular Tool for Membrane Protein Research. Structure 26(2):345-355.e5 (2018)
...Mertens HDT, Ural-Blimke Y, Martinez Molledo M, Svergun DI, Löw C
RgGuinier 5.6 nm
Dmax 16.5 nm
VolumePorod 896 nm3

SASDBZ6 – Draxin

Draxin experimental SAS data
Draxin Rg histogram
Sample: Draxin monomer, Homo sapiens protein
Buffer: 20 mM HEPES 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Aug 19
Structural Basis for Draxin-Modulated Axon Guidance and Fasciculation by Netrin-1 through DCC. Neuron 97(6):1261-1267.e4 (2018)
...Mertens HDT, Svergun DI, Xiao J, Zhang Y, Wang JH, Meijers R
RgGuinier 4.2 nm
Dmax 15.0 nm
VolumePorod 87 nm3

SASDC27 – Saposin nanoparticle (SapNP) SapA:DOPC

saposin-a1,2-dioleoyl-sn-glycero-3-phosphocholine experimental SAS data
DAMMIF model
Sample: saposin-a monomer, Homo sapiens protein
1,2-dioleoyl-sn-glycero-3-phosphocholine other, Synthetic compound other
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Dec 15
Saposin Lipid Nanoparticles: A Highly Versatile and Modular Tool for Membrane Protein Research. Structure 26(2):345-355.e5 (2018)
...Mertens HDT, Ural-Blimke Y, Martinez Molledo M, Svergun DI, Löw C
RgGuinier 4.1 nm
Dmax 11.5 nm
VolumePorod 96 nm3

SASDC37 – Saposin nanoparticle (SapNP) SapA:SoyPI

saposin-aL-α-phosphatidylinositol (soy) experimental SAS data
DAMMIF model
Sample: saposin-a monomer, Homo sapiens protein
L-α-phosphatidylinositol (soy) other, Glycine max other
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Dec 15
Saposin Lipid Nanoparticles: A Highly Versatile and Modular Tool for Membrane Protein Research. Structure 26(2):345-355.e5 (2018)
...Mertens HDT, Ural-Blimke Y, Martinez Molledo M, Svergun DI, Löw C
RgGuinier 3.6 nm
Dmax 8.5 nm
VolumePorod 68 nm3

SASDC47 – Saposin nanoparticle (SapNP) SapA:POPG (SEC-SAXS)

saposin-a1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1'-rac-glycerol) experimental SAS data
DAMMIF model
Sample: saposin-a monomer, Homo sapiens protein
1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1'-rac-glycerol) other, synthetic compound other
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Dec 15
Saposin Lipid Nanoparticles: A Highly Versatile and Modular Tool for Membrane Protein Research. Structure 26(2):345-355.e5 (2018)
...Mertens HDT, Ural-Blimke Y, Martinez Molledo M, Svergun DI, Löw C
RgGuinier 4.1 nm
Dmax 10.5 nm
VolumePorod 69 nm3

SASDC57 – Deoxyuridine 5'-triphosphate nucleotidohydrolase

Deoxyuridine 5'-triphosphate nucleotidohydrolase experimental SAS data
DAMMIF model
Sample: Deoxyuridine 5'-triphosphate nucleotidohydrolase trimer, Homo sapiens protein
Buffer: 50 mM HEPES 300 mM NaCl 5 mM MgCl2, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Jul 9
Structural model of human dUTPase in complex with a novel proteinaceous inhibitor. Sci Rep 8(1):4326 (2018)
...Mertens HDT, Tihanyi B, Nagy GN, Kőhegyi B, Matejka J, Harris MJ, Szabó JE, Papp-Kádár V, Németh-Pongrácz V, Ozohanics O, Vékey K, Svergun DI, Borysik AJ, Vértessy BG
RgGuinier 3.0 nm
Dmax 10.0 nm
VolumePorod 117 nm3

SASDB72 – Death associated protein kinase (Basic Loop mutant)

Death associated protein kinase (Basic Loop mutant) experimental SAS data
NONE model
Sample: Death associated protein kinase (Basic Loop mutant) other, Homo sapiens protein
Buffer: 50 mM HEPES 250 mM NaCl 5mM CaCl2 0.25 mM TCEP 5% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Dec 18
Death-Associated Protein Kinase Activity Is Regulated by Coupled Calcium/Calmodulin Binding to Two Distinct Sites. Structure 24(6):851-61 (2016)
...Mertens HD, Komadina D, Hoffmann JE, Yumerefendi H, Svergun DI, Kursula P, Schultz C, McCarthy AA, Hart DJ, Wilmanns M
RgGuinier 2.4 nm
Dmax 7.5 nm
VolumePorod 78 nm3

SASDC77 – Deoxyuridine 5'-triphosphate nucleotidohydrolase complex with SaPIbov1 pathogenicity island repressor [3:3] (SEC-SAXS)

Deoxyuridine 5'-triphosphate nucleotidohydrolaseSaPIbov1 pathogenicity island repressor experimental SAS data
CORAL model
Sample: Deoxyuridine 5'-triphosphate nucleotidohydrolase trimer, Homo sapiens protein
SaPIbov1 pathogenicity island repressor dimer, Staphylococcus aureus protein
Buffer: 50 mM HEPES 300 mM NaCl 5 mM MgCl2, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Jul 10
Structural model of human dUTPase in complex with a novel proteinaceous inhibitor. Sci Rep 8(1):4326 (2018)
...Mertens HDT, Tihanyi B, Nagy GN, Kőhegyi B, Matejka J, Harris MJ, Szabó JE, Papp-Kádár V, Németh-Pongrácz V, Ozohanics O, Vékey K, Svergun DI, Borysik AJ, Vértessy BG
RgGuinier 4.4 nm
Dmax 17.0 nm
VolumePorod 227 nm3

SASDC87 – Deoxyuridine 5'-triphosphate nucleotidohydrolase complex with SaPIbov1 pathogenicity island repressor [3:2] (SEC-SAXS)

Deoxyuridine 5'-triphosphate nucleotidohydrolaseSaPIbov1 pathogenicity island repressor experimental SAS data
DAMMIF model
Sample: Deoxyuridine 5'-triphosphate nucleotidohydrolase trimer, Homo sapiens protein
SaPIbov1 pathogenicity island repressor dimer, Staphylococcus aureus protein
Buffer: 50 mM HEPES 300 mM NaCl 5 mM MgCl2, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Jul 10
Structural model of human dUTPase in complex with a novel proteinaceous inhibitor. Sci Rep 8(1):4326 (2018)
...Mertens HDT, Tihanyi B, Nagy GN, Kőhegyi B, Matejka J, Harris MJ, Szabó JE, Papp-Kádár V, Németh-Pongrácz V, Ozohanics O, Vékey K, Svergun DI, Borysik AJ, Vértessy BG
RgGuinier 3.8 nm
Dmax 14.0 nm
VolumePorod 190 nm3

SASDC97 – Ethylene Receptor 1 (DHp + CA + RD domains)

Ethylene receptor 1 dimerization histidine phosphotransfer + catalytic ATP-binding + receiver domains experimental SAS data
Ethylene receptor 1 dimerization histidine phosphotransfer + catalytic ATP-binding + receiver domains Kratky plot
Sample: Ethylene receptor 1 dimerization histidine phosphotransfer + catalytic ATP-binding + receiver domains dimer, Arabidopsis thaliana protein
Buffer: 20 mM Tris 150 mM NaCl 1 mM DTT 5 mM ADP, pH: 8.8
Experiment: SAXS data collected at EMBL X33, DORIS III on 2010 Oct 11
Structural model of the cytosolic domain of the plant ethylene receptor 1 (ETR1). J Biol Chem 290(5):2644-58 (2015)
...Mertens HD, Mueller-Dieckmann J
RgGuinier 4.0 nm
Dmax 13.9 nm
VolumePorod 144 nm3

SASDCA7 – Ethylene Receptor 1 (DHp + CA domains)

Ethylene receptor 1 dimerization histidine phosphotransfer + catalytic ATP-binding domains experimental SAS data
Ethylene receptor 1 dimerization histidine phosphotransfer + catalytic ATP-binding domains Kratky plot
Sample: Ethylene receptor 1 dimerization histidine phosphotransfer + catalytic ATP-binding domains dimer, Arabidopsis thaliana protein
Buffer: 20 mM Tris 150 mM NaCl 1 mM DTT 5 mM ADP, pH: 8.8
Experiment: SAXS data collected at EMBL X33, DORIS III on 2010 Oct 11
Structural model of the cytosolic domain of the plant ethylene receptor 1 (ETR1). J Biol Chem 290(5):2644-58 (2015)
...Mertens HD, Mueller-Dieckmann J
RgGuinier 2.7 nm
Dmax 8.7 nm
VolumePorod 74 nm3

SASDAD7 – Structure of a complex between full length and truncated CTP1L endolysin

Endolysin  experimental SAS data
CRYSOL model
Sample: Endolysin other, Clostridium phage phiCTP1 protein
Buffer: 20 mM HEPES, pH: 7.4
Experiment: SAXS data collected at EMBL X33, DORIS III on 2011 Mar 17
Crystal Structure of the CTP1L Endolysin Reveals How Its Activity Is Regulated by a Secondary Translation Product. J Biol Chem 291(10):4882-93 (2016)
...Mertens HD, Thompson A, Krijgsveld J, Svergun DI, Gómez-Torres N, Garde S, Uetrecht C, Narbad A, Mayer MJ, Meijers R
RgGuinier 3.7 nm
Dmax 13.8 nm
VolumePorod 95 nm3

SASDAE7 – Structure of a complex between full length and truncated CS74L endolysin

Endolysin CS74L  experimental SAS data
DAMMIF model
Sample: Endolysin CS74L other, Clostridium phage phi8074-B1 protein
Buffer: 20 mM HEPES, pH: 7.4
Experiment: SAXS data collected at EMBL X33, DORIS III on 2011 Mar 17
Crystal Structure of the CTP1L Endolysin Reveals How Its Activity Is Regulated by a Secondary Translation Product. J Biol Chem 291(10):4882-93 (2016)
...Mertens HD, Thompson A, Krijgsveld J, Svergun DI, Gómez-Torres N, Garde S, Uetrecht C, Narbad A, Mayer MJ, Meijers R
RgGuinier 3.6 nm
Dmax 14.0 nm
VolumePorod 79 nm3

SASDEW4 – ACA8 complex with Calmodulin (hydrogenated) in stealth nanodisc

Membrane scaffold protein 1D1 (deuterated, 75%)1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl)Calcium-transporting ATPase 8, plasma membrane-typeCalmodulin-7 experimental SAS data
Membrane scaffold protein 1D1 (deuterated, 75%) 1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl) Calcium-transporting ATPase 8, plasma membrane-type Calmodulin-7 Kratky plot
Sample: Membrane scaffold protein 1D1 (deuterated, 75%) dimer, None protein
1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl) unknown, Escherichia coli. (AL95 ... other
Calcium-transporting ATPase 8, plasma membrane-type monomer, Arabidopsis thaliana protein
Calmodulin-7 monomer, Arabidopsis thaliana protein
Buffer: 30 mM Tris, 150 mM NaCl, 1mM MgCl2, 1 mM CaCl2, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Sep 8
Structural basis for activation of plasma-membrane Ca2+-ATPase by calmodulin Communications Biology 1(1) (2018)
...Mertens H, Maric S, Moulin M, Haertlein M, Busch S, Forsyth V, Svergun D, Uetrecht C, Tidow H
RgGuinier 5.9 nm
Dmax 22.0 nm
VolumePorod 805 nm3

SASDC67 – SaPIbov1 pathogenicity island repressor

SaPIbov1 pathogenicity island repressor experimental SAS data
OTHER model
Sample: SaPIbov1 pathogenicity island repressor dimer, Staphylococcus aureus protein
Buffer: 50 mM HEPES 300 mM NaCl 5 mM MgCl2, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Jul 9
Structural model of human dUTPase in complex with a novel proteinaceous inhibitor. Sci Rep 8(1):4326 (2018)
...Mertens HDT, Tihanyi B, Nagy GN, Kőhegyi B, Matejka J, Harris MJ, Szabó JE, Papp-Kádár V, Németh-Pongrácz V, Ozohanics O, Vékey K, Svergun DI, Borysik AJ, Vértessy BG
RgGuinier 3.3 nm
Dmax 10.5 nm
VolumePorod 100 nm3

SASDCM9 – Ammonium transporter Ks-Amt5

ammonium sensor/transducer experimental SAS data
CORAL model
Sample: ammonium sensor/transducer trimer, Candidatus Kuenenia stuttgartiensis protein
Buffer: 20 mM Tris/HCl,100 mM NaCl, 5%(w/v) glycerol, 0.09%(w/v) CYMAL-5, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Mar 1
Signaling ammonium across membranes through an ammonium sensor histidine kinase. Nat Commun 9(1):164 (2018)
...Mertens H, Svergun D, Baumstark MW, Lunin VY, Jetten MSM, Andrade SLA
RgGuinier 4.8 nm
Dmax 17.0 nm
VolumePorod 511 nm3

SASDDM9 – lipoprotein lipase–GPIHBP1 complex

Lipoprotein lipaseGlycosylphosphatidylinositol-anchored high density lipoprotein-binding protein 1 experimental SAS data
DAMMIF model
Sample: Lipoprotein lipase dimer, Homo sapiens protein
Glycosylphosphatidylinositol-anchored high density lipoprotein-binding protein 1 dimer, Homo sapiens protein
Buffer: 10 mM BisTris, 150 mM NaCl, 4 mM CaCl2, 10% glycerol, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Dec 2
Structure of the lipoprotein lipase–GPIHBP1 complex that mediates plasma triglyceride hydrolysis
Haydyn Mertens
RgGuinier 5.5 nm
Dmax 19.0 nm
VolumePorod 319 nm3

SASDCZ6 – DtpA SapNP (SapA:POPS) (SEC-SAXS)

saposin-a1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serineDipeptide and tripeptide permease A experimental SAS data
DAMMIF model
Sample: saposin-a monomer, Homo sapiens protein
1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serine other, Synthetic compound other
Dipeptide and tripeptide permease A monomer, Escherichia coli (strain ... protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 May 4
Saposin Lipid Nanoparticles: A Highly Versatile and Modular Tool for Membrane Protein Research. Structure 26(2):345-355.e5 (2018)
...Mertens HDT, Ural-Blimke Y, Martinez Molledo M, Svergun DI, Löw C
RgGuinier 4.0 nm
Dmax 13.5 nm
VolumePorod 288 nm3

SASDEU4 – ACA8 complex with Calmodulin (hydrogenated) in stealth nanodisc (SANS, 100% D2O)

Membrane scaffold protein 1D1 (deuterated, 75%)1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl)Calcium-transporting ATPase 8, plasma membrane-typeCalmodulin-7 experimental SAS data
Membrane scaffold protein 1D1 (deuterated, 75%) 1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl) Calcium-transporting ATPase 8, plasma membrane-type Calmodulin-7 Kratky plot
Sample: Membrane scaffold protein 1D1 (deuterated, 75%) dimer, None protein
1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl) unknown, Escherichia coli. (AL95 ... other
Calcium-transporting ATPase 8, plasma membrane-type monomer, Arabidopsis thaliana protein
Calmodulin-7 monomer, Arabidopsis thaliana protein
Buffer: 30 mM Tris, 150 mM NaCl, 1mM MgCl2, 1 mM CaCl2, pH: 7.5
Experiment: SANS data collected at SANS-1, Heinz Maier-Leibnitz Zentrum on 2017 Aug 28
Structural basis for activation of plasma-membrane Ca2+-ATPase by calmodulin Communications Biology 1(1) (2018)
...Mertens H, Maric S, Moulin M, Haertlein M, Busch S, Forsyth V, Svergun D, Uetrecht C, Tidow H
RgGuinier 4.8 nm
Dmax 18.0 nm
VolumePorod 297 nm3

SASDES4 – ACA8 in stealth nanodisc (SANS, 100% D2O)

Membrane scaffold protein 1D1 (deuterated, 75%)1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl)Calcium-transporting ATPase 8, plasma membrane-type experimental SAS data
Membrane scaffold protein 1D1 (deuterated, 75%) 1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl) Calcium-transporting ATPase 8, plasma membrane-type Kratky plot
Sample: Membrane scaffold protein 1D1 (deuterated, 75%) dimer, None protein
1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl) unknown, Escherichia coli. (AL95 ... other
Calcium-transporting ATPase 8, plasma membrane-type monomer, Arabidopsis thaliana protein
Buffer: 30 mM Tris, 150 mM NaCl, 1mM MgCl2, 1 mM CaCl2, pH: 7.5
Experiment: SANS data collected at SANS-1, Heinz Maier-Leibnitz Zentrum on 2017 Aug 28
Structural basis for activation of plasma-membrane Ca2+-ATPase by calmodulin Communications Biology 1(1) (2018)
...Mertens H, Maric S, Moulin M, Haertlein M, Busch S, Forsyth V, Svergun D, Uetrecht C, Tidow H
RgGuinier 4.0 nm
Dmax 13.0 nm
VolumePorod 202 nm3

SASDAT4 – uPAR wild-type

Urokinase plasminogen activator surface receptor experimental SAS data
DAMMIF model
Sample: Urokinase plasminogen activator surface receptor monomer, Homo sapiens protein
Buffer: 25 mM Sodium Phosphate 5 % Glycerol 50 mM NaSO4, pH: 7.2
Experiment: SAXS data collected at EMBL X33, DORIS III on 2010 Jun 10
A flexible multidomain structure drives the function of the urokinase-type plasminogen activator receptor (uPAR). J Biol Chem 287(41):34304-15 (2012)
Mertens HD, Kjaergaard M, Mysling S, Gårdsvoll H, Jørgensen TJ, Svergun DI, Ploug M
RgGuinier 2.8 nm
Dmax 9.7 nm
VolumePorod 69 nm3

SASDAU4 – uPAR H47C/N259C

Urokinase plasminogen activator surface receptor experimental SAS data
DAMMIF model
Sample: Urokinase plasminogen activator surface receptor monomer, Homo sapiens protein
Buffer: 25 mM Sodium Phosphate 5 % Glycerol 50 mM NaSO4, pH: 7.2
Experiment: SAXS data collected at EMBL X33, DORIS III on 2010 Jun 10
A flexible multidomain structure drives the function of the urokinase-type plasminogen activator receptor (uPAR). J Biol Chem 287(41):34304-15 (2012)
Mertens HD, Kjaergaard M, Mysling S, Gårdsvoll H, Jørgensen TJ, Svergun DI, Ploug M
RgGuinier 2.2 nm
Dmax 7.0 nm
VolumePorod 57 nm3

SASDAV4 – uPAR wild-type AE105 complex

Urokinase plasminogen activator surface receptorsynthetic peptide AE105 experimental SAS data
Urokinase plasminogen activator surface receptor synthetic peptide AE105 Kratky plot
Sample: Urokinase plasminogen activator surface receptor monomer, Homo sapiens protein
synthetic peptide AE105 monomer, protein
Buffer: 25 mM Sodium Phosphate 5 % Glycerol 50 mM NaSO4, pH: 7.2
Experiment: SAXS data collected at EMBL X33, DORIS III on 2010 Jun 10
A flexible multidomain structure drives the function of the urokinase-type plasminogen activator receptor (uPAR). J Biol Chem 287(41):34304-15 (2012)
Mertens HD, Kjaergaard M, Mysling S, Gårdsvoll H, Jørgensen TJ, Svergun DI, Ploug M
RgGuinier 2.5 nm
Dmax 8.5 nm
VolumePorod 61 nm3

SASDAW4 – uPAR wild-type AE234 complex

Urokinase plasminogen activator surface receptorsynthetic peptide AE234 experimental SAS data
Urokinase plasminogen activator surface receptor synthetic peptide AE234 Kratky plot
Sample: Urokinase plasminogen activator surface receptor monomer, Homo sapiens protein
synthetic peptide AE234 monomer, protein
Buffer: 25 mM Sodium Phosphate 5 % Glycerol 50 mM NaSO4, pH: 7.2
Experiment: SAXS data collected at EMBL X33, DORIS III on 2010 Jun 10
A flexible multidomain structure drives the function of the urokinase-type plasminogen activator receptor (uPAR). J Biol Chem 287(41):34304-15 (2012)
Mertens HD, Kjaergaard M, Mysling S, Gårdsvoll H, Jørgensen TJ, Svergun DI, Ploug M
RgGuinier 2.4 nm
Dmax 8.3 nm
VolumePorod 57 nm3

SASDAX4 – uPAR wild-type ATF complex

Urokinase plasminogen activator surface receptorUrokinase-type plasminogen activator experimental SAS data
Urokinase plasminogen activator surface receptor Urokinase-type plasminogen activator Kratky plot
Sample: Urokinase plasminogen activator surface receptor monomer, Homo sapiens protein
Urokinase-type plasminogen activator monomer, Homo sapiens protein
Buffer: 25 mM Sodium Phosphate 5 % Glycerol 50 mM NaSO4, pH: 7.2
Experiment: SAXS data collected at EMBL X33, DORIS III on 2010 Jun 10
A flexible multidomain structure drives the function of the urokinase-type plasminogen activator receptor (uPAR). J Biol Chem 287(41):34304-15 (2012)
Mertens HD, Kjaergaard M, Mysling S, Gårdsvoll H, Jørgensen TJ, Svergun DI, Ploug M
RgGuinier 2.8 nm
Dmax 9.4 nm
VolumePorod 102 nm3

SASDAY4 – fH1015

Factor H CCP modules 10 to 15 experimental SAS data
DAMMIF model
Sample: Factor H CCP modules 10 to 15 monomer, Homo sapiens protein
Buffer: 50 mM Potassium Phosphate, pH: 7.4
Experiment: SAXS data collected at EMBL X33, DORIS III on 2008 Dec 2
The central portion of factor H (modules 10-15) is compact and contains a structurally deviant CCP module. J Mol Biol 395(1):105-22 (2010)
...Mertens HD, Guariento M, Soares DC, Uhrin D, Rowe AJ, Svergun DI, Barlow PN
RgGuinier 3.0 nm
Dmax 10.4 nm
VolumePorod 68 nm3

SASDAZ4 – fH1114

Factor H CCP modules 11 to 14 experimental SAS data
DAMMIF model
Sample: Factor H CCP modules 11 to 14 monomer, Homo sapiens protein
Buffer: 50 mM Potassium Phosphate, pH: 7.4
Experiment: SAXS data collected at EMBL X33, DORIS III on 2008 Dec 2
The central portion of factor H (modules 10-15) is compact and contains a structurally deviant CCP module. J Mol Biol 395(1):105-22 (2010)
...Mertens HD, Guariento M, Soares DC, Uhrin D, Rowe AJ, Svergun DI, Barlow PN
RgGuinier 3.0 nm
Dmax 10.5 nm
VolumePorod 38 nm3

SASDA25 – fh1213

Factor H CCP modules 12 to 13 experimental SAS data
CRYSOL model
Sample: Factor H CCP modules 12 to 13 monomer, Homo sapiens protein
Buffer: 50 mM Potassium Phosphate, pH: 7.4
Experiment: SAXS data collected at EMBL X33, DORIS III on 2008 Dec 2
The central portion of factor H (modules 10-15) is compact and contains a structurally deviant CCP module. J Mol Biol 395(1):105-22 (2010)
...Mertens HD, Guariento M, Soares DC, Uhrin D, Rowe AJ, Svergun DI, Barlow PN
RgGuinier 2.0 nm
Dmax 7.1 nm
VolumePorod 20 nm3

SASDA85 – CHD4 (PP-CC-AH-D)

Human Chromatin Remodeler CHD4 (363-1353) experimental SAS data
DAMMIF model
Sample: Human Chromatin Remodeler CHD4 (363-1353) monomer, Homo sapiens protein
Buffer: 50 mM HEPES 5% Glycerol 300 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III on 2010 Nov 13
The PHD and chromo domains regulate the ATPase activity of the human chromatin remodeler CHD4. J Mol Biol 422(1):3-17 (2012)
...Mertens HD, Deery MJ, Zhang W, Pham P, Du X, Bartke T, Zhang W, Edlich C, Berridge G, Chen Y, Burgess-Brown NA, Kouzarides T, Wiechens N, Owen-Hughes T, Svergun DI, Gileadi O, Laue ED
RgGuinier 4.9 nm
Dmax 17.4 nm

SASDA95 – CHD4 (CC-AH-D)

Human Chromatin Remodeler CHD4 (494-1353) experimental SAS data
DAMMIF model
Sample: Human Chromatin Remodeler CHD4 (494-1353) monomer, Homo sapiens protein
Buffer: 50 mM HEPES 50 mM KCl, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III on 2010 Nov 13
The PHD and chromo domains regulate the ATPase activity of the human chromatin remodeler CHD4. J Mol Biol 422(1):3-17 (2012)
...Mertens HD, Deery MJ, Zhang W, Pham P, Du X, Bartke T, Zhang W, Edlich C, Berridge G, Chen Y, Burgess-Brown NA, Kouzarides T, Wiechens N, Owen-Hughes T, Svergun DI, Gileadi O, Laue ED
RgGuinier 4.0 nm
Dmax 14.5 nm

SASDAA5 – CHD4 (AH)

Human Chromatin Remodeler CHD4 (685-1233) experimental SAS data
DAMMIF model
Sample: Human Chromatin Remodeler CHD4 (685-1233) monomer, Homo sapiens protein
Buffer: 50 mM HEPES 50 mM KCl, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III on 2010 Nov 13
The PHD and chromo domains regulate the ATPase activity of the human chromatin remodeler CHD4. J Mol Biol 422(1):3-17 (2012)
...Mertens HD, Deery MJ, Zhang W, Pham P, Du X, Bartke T, Zhang W, Edlich C, Berridge G, Chen Y, Burgess-Brown NA, Kouzarides T, Wiechens N, Owen-Hughes T, Svergun DI, Gileadi O, Laue ED
RgGuinier 4.0 nm
Dmax 14.5 nm

SASDAB5 – CHD4 (PP-CC)

Human Chromatin Remodeler CHD4 (363-682) experimental SAS data
DAMMIF model
Sample: Human Chromatin Remodeler CHD4 (363-682) monomer, Homo sapiens protein
Buffer: 50 mM HEPES 50 mM KCl, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III on 2010 Nov 13
The PHD and chromo domains regulate the ATPase activity of the human chromatin remodeler CHD4. J Mol Biol 422(1):3-17 (2012)
...Mertens HD, Deery MJ, Zhang W, Pham P, Du X, Bartke T, Zhang W, Edlich C, Berridge G, Chen Y, Burgess-Brown NA, Kouzarides T, Wiechens N, Owen-Hughes T, Svergun DI, Gileadi O, Laue ED
RgGuinier 3.0 nm
Dmax 10.5 nm

SASDDC5 – MsbA in stealth nanodisc (SANS, 100% D2O) + 1 mM ADP

Lipid A export ATP-binding/permease protein MsbAMembrane scaffold protein 1D1 (deuterated, 75%)1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl) experimental SAS data
Lipid A export ATP-binding/permease protein MsbA Membrane scaffold protein 1D1 (deuterated, 75%) 1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl) Kratky plot
Sample: Lipid A export ATP-binding/permease protein MsbA dimer, Escherichia coli (strain ... protein
Membrane scaffold protein 1D1 (deuterated, 75%) dimer, None protein
1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl) unknown, Escherichia coli. (AL95 ... other
Buffer: 30 mM Tris, 150 mM NaCl, 1 mM ADP, pH: 7.5
Experiment: SANS data collected at D11, ILL on 2017 Mar 9
Conformational States of ABC Transporter MsbA in a Lipid Environment Investigated by Small-Angle Scattering Using Stealth Carrier Nanodiscs. Structure 26(8):1072-1079.e4 (2018)
...Mertens HD, Moulin M, Haertlein M, Prevost S, Svergun DI, Busch S, Forsyth VT, Tidow H
RgGuinier 3.9 nm
Dmax 12.5 nm
VolumePorod 173 nm3

SASDDD5 – MsbA in stealth nanodisc (SAXS)

Lipid A export ATP-binding/permease protein MsbAMembrane scaffold protein 1D1 (deuterated, 75%)1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl) experimental SAS data
Lipid A export ATP-binding/permease protein MsbA Membrane scaffold protein 1D1 (deuterated, 75%) 1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl) Kratky plot
Sample: Lipid A export ATP-binding/permease protein MsbA dimer, Escherichia coli (strain ... protein
Membrane scaffold protein 1D1 (deuterated, 75%) dimer, None protein
1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl) unknown, Escherichia coli. (AL95 ... other
Buffer: 30 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Sep 8
Conformational States of ABC Transporter MsbA in a Lipid Environment Investigated by Small-Angle Scattering Using Stealth Carrier Nanodiscs. Structure 26(8):1072-1079.e4 (2018)
...Mertens HD, Moulin M, Haertlein M, Prevost S, Svergun DI, Busch S, Forsyth VT, Tidow H
RgGuinier 4.8 nm
Dmax 16.0 nm
VolumePorod 607 nm3

SASDAE5 – CYNEX4

CYNEX4 FRET probe, (eYFP-AnnexinA4-eCFP) experimental SAS data
DAMMIF model
Sample: CYNEX4 FRET probe, (eYFP-AnnexinA4-eCFP) monomer, Homo sapiens protein
Buffer: 50 mM HEPES 150 mM NaCl 1 mM EGTA, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III on 2010 Sep 11
Conformational analysis of a genetically encoded FRET biosensor by SAXS. Biophys J 102(12):2866-75 (2012)
Mertens HD, Piljić A, Schultz C, Svergun DI
RgGuinier 3.7 nm
Dmax 12.8 nm
VolumePorod 135 nm3

SASDAF5 – CYNEX4-T266D

CYNEX4 FRET probe, (eYFP-AnnexinA4-eCFP) T266D mutant experimental SAS data
DAMMIF model
Sample: CYNEX4 FRET probe, (eYFP-AnnexinA4-eCFP) T266D mutant monomer, Homo sapiens protein
Buffer: 50 mM HEPES 50 mM KCl, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III on 2010 Sep 11
Conformational analysis of a genetically encoded FRET biosensor by SAXS. Biophys J 102(12):2866-75 (2012)
Mertens HD, Piljić A, Schultz C, Svergun DI
RgGuinier 4.1 nm
Dmax 14.4 nm
VolumePorod 146 nm3

SASDEV4 – ACA8 protein (apo) in stealth nanodisc

Membrane scaffold protein 1D1 (deuterated, 75%)1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl)Calcium-transporting ATPase 8, plasma membrane-type experimental SAS data
Membrane scaffold protein 1D1 (deuterated, 75%) 1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl) Calcium-transporting ATPase 8, plasma membrane-type Kratky plot
Sample: Membrane scaffold protein 1D1 (deuterated, 75%) dimer, None protein
1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl) unknown, Escherichia coli. (AL95 ... other
Calcium-transporting ATPase 8, plasma membrane-type monomer, Arabidopsis thaliana protein
Buffer: 30 mM Tris, 150 mM NaCl, 1mM MgCl2, 1 mM CaCl2, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Sep 8
Structural basis for activation of plasma-membrane Ca2+-ATPase by calmodulin Communications Biology 1(1) (2018)
...Mertens H, Maric S, Moulin M, Haertlein M, Busch S, Forsyth V, Svergun D, Uetrecht C, Tidow H
RgGuinier 5.3 nm
Dmax 20.0 nm
VolumePorod 626 nm3

SASDAJ5 – Annexin-A4

Annexin-A4 experimental SAS data
CRYSOL model
Sample: Annexin-A4 monomer, Homo sapiens protein
Buffer: 50 mM HEPES 50 mM KCl, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III on 2010 Sep 11
Conformational analysis of a genetically encoded FRET biosensor by SAXS. Biophys J 102(12):2866-75 (2012)
Mertens HD, Piljić A, Schultz C, Svergun DI
RgGuinier 2.4 nm
Dmax 7.6 nm
VolumePorod 54 nm3

SASDAL5 – Clostridium difficile bacteriophage 27 endolysin dimer, CD27L

Clostridium difficile bacteriophage 27 endolysin experimental SAS data
NONE model
Sample: Clostridium difficile bacteriophage 27 endolysin dimer, Clostridioides difficile protein
Buffer: 20 mM HEPES 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III on 2011 Mar 17
The CD27L and CTP1L endolysins targeting Clostridia contain a built-in trigger and release factor. PLoS Pathog 10(7):e1004228 (2014)
...Mertens HD, Garefalaki V, Jeffries CM, Thompson A, Lemke EA, Svergun DI, Mayer MJ, Narbad A, Meijers R
RgGuinier 3.3 nm
Dmax 10.6 nm
VolumePorod 72 nm3

SASDAM5 – Clostridium difficile bacteriophage 27 endolysin dimer C238R mutant, CD27L-C238R

Clostridium difficile bacteriophage 27 endolysin C238R mutant experimental SAS data
DAMMIF model
Sample: Clostridium difficile bacteriophage 27 endolysin C238R mutant dimer, Clostridioides difficile protein
Buffer: 20 mM HEPES 500 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Jun 21
The CD27L and CTP1L endolysins targeting Clostridia contain a built-in trigger and release factor. PLoS Pathog 10(7):e1004228 (2014)
...Mertens HD, Garefalaki V, Jeffries CM, Thompson A, Lemke EA, Svergun DI, Mayer MJ, Narbad A, Meijers R
RgGuinier 4.2 nm
Dmax 14.7 nm
VolumePorod 92 nm3

SASDCN9 – Ks-Amt5-HK (apo)

Ammonium transporter histidine kinase domain experimental SAS data
Ammonium transporter histidine kinase domain Kratky plot
Sample: Ammonium transporter histidine kinase domain monomer, Candidatus Kuenenia stuttgartiensis protein
Buffer: 20 mM Tris/HCl,100 mM NaCl, 5%(w/v) glycerol, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Mar 2
Signaling ammonium across membranes through an ammonium sensor histidine kinase. Nat Commun 9(1):164 (2018)
...Mertens H, Svergun D, Baumstark MW, Lunin VY, Jetten MSM, Andrade SLA
RgGuinier 2.7 nm
Dmax 10.0 nm
VolumePorod 71 nm3

SASDCP9 – Ks-Amt5-HK (+10uM APPCP)

Ammonium transporter histidine kinase domain experimental SAS data
Ammonium transporter histidine kinase domain Kratky plot
Sample: Ammonium transporter histidine kinase domain monomer, Candidatus Kuenenia stuttgartiensis protein
Buffer: 20 mM Tris/HCl,100 mM NaCl, 5%(w/v) glycerol, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Mar 2
Signaling ammonium across membranes through an ammonium sensor histidine kinase. Nat Commun 9(1):164 (2018)
...Mertens H, Svergun D, Baumstark MW, Lunin VY, Jetten MSM, Andrade SLA
RgGuinier 2.3 nm
Dmax 8.0 nm
VolumePorod 53 nm3

SASDCQ9 – Ks-Amt5-HK (+10uM ATP)

Ammonium transporter histidine kinase domain experimental SAS data
Ammonium transporter histidine kinase domain Kratky plot
Sample: Ammonium transporter histidine kinase domain monomer, Candidatus Kuenenia stuttgartiensis protein
Buffer: 20 mM Tris/HCl,100 mM NaCl, 5%(w/v) glycerol, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Mar 2
Signaling ammonium across membranes through an ammonium sensor histidine kinase. Nat Commun 9(1):164 (2018)
...Mertens H, Svergun D, Baumstark MW, Lunin VY, Jetten MSM, Andrade SLA
RgGuinier 2.2 nm
Dmax 7.7 nm
VolumePorod 53 nm3

SASDCS9 – Ammonium transporter Ks-Amt5 (Batch)

ammonium sensor/transducer experimental SAS data
ammonium sensor/transducer Kratky plot
Sample: ammonium sensor/transducer trimer, Candidatus Kuenenia stuttgartiensis protein
Buffer: 20 mM Tris/HCl,100 mM NaCl, 5%(w/v) glycerol, 0.09%(w/v) CYMAL-5, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Mar 2
Signaling ammonium across membranes through an ammonium sensor histidine kinase. Nat Commun 9(1):164 (2018)
...Mertens H, Svergun D, Baumstark MW, Lunin VY, Jetten MSM, Andrade SLA
RgGuinier 4.9 nm
Dmax 17.0 nm
VolumePorod 482 nm3

SASDDB5 – MsbA in stealth nanodisc (SANS, 100% D2O)

Lipid A export ATP-binding/permease protein MsbAMembrane scaffold protein 1D1 (deuterated, 75%)1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl) experimental SAS data
SASREF CV model
Sample: Lipid A export ATP-binding/permease protein MsbA dimer, Escherichia coli (strain ... protein
Membrane scaffold protein 1D1 (deuterated, 75%) dimer, None protein
1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl) unknown, Escherichia coli. (AL95 ... other
Buffer: 30 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SANS data collected at D11, ILL on 2017 Mar 9
Conformational States of ABC Transporter MsbA in a Lipid Environment Investigated by Small-Angle Scattering Using Stealth Carrier Nanodiscs. Structure 26(8):1072-1079.e4 (2018)
...Mertens HD, Moulin M, Haertlein M, Prevost S, Svergun DI, Busch S, Forsyth VT, Tidow H
RgGuinier 4.0 nm
Dmax 13.0 nm
VolumePorod 189 nm3