data from solutions of
Catalase in PBS
PBS, pH 7.4
were collected on the
EMBL X33 camera
at the DORIS III storage ring
using a Pilatus 1M-W detector
at a sample-detector distance of 2.7 m and
at a wavelength of λ = 0.15 nm
, the range of momentum transfer 0.087 < s < 6.014 nm-1 was covered
(l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
Solute concentrations ranging between 0.1 and 6.9 mg/ml were measured
15 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The low angle data collected at lower concentrations were extrapolated to infinite dilution and merged with the higher concentration data to yield the final composite scattering curve.