Standard proteins

Darja Ruskule.

SASDA92 – Catalase in PBS

Catalase
MWI(0) 254 kDa
MWexpected 240 kDa
VPorod 296 nm3
log I(s) 2.22×102 2.22×101 2.22×100 2.22×10-1
Catalase small angle scattering data  s, nm-1
ln I(s)
Catalase Guinier plot ln 2.22×102 Rg: 4.1 nm 0 (4.1 nm)-2 s2
(sRg)2I(s)/I(0)
Catalase Kratky plot 1.104 0 3 sRg
p(r)
Catalase pair distance distribution function Rg: 3.7 nm 0 Dmax: 10.9 nm

Experimental data validation


Fits and models


log I(s)
 s, nm-1
Catalase PDB model
log I(s)
 s, nm-1
Catalase DAMMIF model
Synchrotron SAXS data from solutions of Catalase in PBS in PBS, pH 7.4 were collected on the X33 camera on the storage ring DORIS III (Hamburg, Germany). Using a Pilatus 1M-W detector at a sample-detector distance of 2.7 m and at a wavelength of λ = 0.15 nm , the range of momentum transfer 0.087 < s < 6.014 nm-1 was covered (s = 4π sin θ/λ, where 2θ is the scattering angle). Solute concentrations ranging between 0.1 and 6.9 mg/ml were measured at 10°C. Eight successive 15 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the different curves were scaled for protein concentration. The low angle data collected at lower concentrations were extrapolated to infinite dilution and merged with the higher concentration data to yield the final composite scattering curve.

Catalase
Mol. type   Protein
Organism   Bos taurus
Olig. state   Tetramer
Mon. MW   59.9 kDa
 
UniProt   P00432
Sequence   FASTA
 
PDB code   4BLC