Synchrotron SAXS
data from solutions of
Catalase in PBS
in
PBS, pH 7.4
were collected
on the
EMBL X33 beam line
at the DORIS III storage ring
(Hamburg, Germany)
using a Pilatus 1M-W detector
at a sample-detector distance of 2.7 m and
at a wavelength of λ = 0.15 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
Solute concentrations ranging between 0.1 and 6.9 mg/ml were measured
at 10°C.
Eight successive
15 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The low angle data collected at lower concentrations were extrapolated to infinite dilution and merged with the higher concentration data to yield the final composite scattering curve.
|
Catalase
|
Mol. type |
|
Protein |
Organism |
|
Bos taurus |
Olig. state |
|
Tetramer |
Mon. MW |
|
59.9 kDa |
|
UniProt |
|
P00432
|
Sequence |
|
FASTA |
|
PDB ID
|
|
4BLC
|
|
|