Standard proteins

Darja Ruskule.

SASDAA2 – Conalbumin in PBS

Ovotransferrin
MWI(0) 68 kDa
MWexpected 78 kDa
VPorod 117 nm3
log I(s) 8.28×101 8.28×100 8.28×10-1 8.28×10-2
Ovotransferrin small angle scattering data  s, nm-1
ln I(s)
Ovotransferrin Guinier plot ln 8.28×101 Rg: 3.8 nm 0 (3.8 nm)-2 s2
(sRg)2I(s)/I(0)
Ovotransferrin Kratky plot 1.104 0 √3 sRg
p(r)
Ovotransferrin pair distance distribution function Rg: 3.4 nm 0 Dmax: 11 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Ovotransferrin DAMMIF model
log I(s)
 s, nm-1
Ovotransferrin PDB (PROTEIN DATA BANK) model
Synchrotron SAXS data from solutions of Conalbumin in PBS in PBS, pH 7.4 were collected on the EMBL X33 beam line at the DORIS III storage ring (Hamburg, Germany) using a Pilatus 1M-W detector at a sample-detector distance of 2.7 m and at a wavelength of λ = 0.15 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 12.17 mg/ml was measured at 10°C. Eight successive 15 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.

Concentration min = UNKNOWN

Tags: benchmark X33
Ovotransferrin
Mol. type   Protein
Organism   Gallus gallus
Olig. state   Monomer
Mon. MW   77.8 kDa
 
UniProt   P02789
Sequence   FASTA
 
PDB ID   1RYX