Standard proteins

Darja Ruskule.

SASDAC2 – Lyz in Sodium Acetate

Lysozyme C
MWI(0) 11 kDa
MWexpected 14 kDa
VPorod 17 nm3
log I(s) 1.24×101 1.24×100 1.24×10-1 1.24×10-2
Lysozyme C small angle scattering data  s, nm-1
ln I(s)
Lysozyme C Guinier plot ln 1.24×101 Rg: 1.5 nm 0 (1.5 nm)-2 s2
(sRg)2I(s)/I(0)
Lysozyme C Kratky plot 1.104 0 3 sRg
p(r)
Lysozyme C pair distance distribution function Rg: 1.4 nm 0 Dmax: 4 nm

Data validation


Fits and models


log I(s)
 s, nm-1
Lysozyme C CRYSOL model

log I(s)
 s, nm-1
Lysozyme C DAMMIF model

Synchrotron SAXS data from solutions of Lyz in Sodium Acetate in 40 mM Sodium Acetate, pH 3.8 were collected on the EMBL X33 camera at the DORIS III storage ring (Hamburg, Germany) using a Pilatus 1M-W detector at a sample-detector distance of 2.7 m and at a wavelength of λ = 0.15 nm , the range of momentum transfer 0.087 < s < 6.014 nm-1 was covered (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 8.09 mg/ml was measured at 10°C. Eight successive 15 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Lysozyme C (Lys)
Mol. type   Protein
Organism   Gallus gallus
Olig. state   Monomer
Mon. MW   16.2 kDa
 
UniProt   P00698
Sequence   FASTA