SdsA polymorph isolation and improvement of their crystal quality using nonconventional crystallization techniques

De la Mora E, Flores-Hernández E, Jakoncic J, Stojanoff V, Siliqi D, Sánchez-Puig N, Moreno A, Journal of Applied Crystallography 48(5):1551-1559 (2015) DOI

SASDAC8 – SDS hydrolase SdsA1

Pseudomonas aeruginosa SDS hydrolase SdsA1
MWexperimental 140 kDa
MWexpected 145 kDa
VPorod 261 nm3
log I(s) 1.59×103 1.59×102 1.59×101 1.59×100
Pseudomonas aeruginosa SDS hydrolase SdsA1 small angle scattering data  s, nm-1
ln I(s)
Pseudomonas aeruginosa SDS hydrolase SdsA1 Guinier plot ln 1.59×103 Rg: 3.6 nm 0 (3.6 nm)-2 s2
(sRg)2I(s)/I(0)
Pseudomonas aeruginosa SDS hydrolase SdsA1 Kratky plot 1.104 0 √3 sRg
p(r)
Pseudomonas aeruginosa SDS hydrolase SdsA1 pair distance distribution function Rg: 3.7 nm 0 Dmax: 16.9 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Pseudomonas aeruginosa SDS hydrolase SdsA1 CRYSOL model
X-ray synchrotron radiation scattering data from solutions of Pseudomonas aeruginosa SDS hydrolase SdsA1 in 50 mM HEPES were collected on the X9A camera on the storage ring NSLS (Brookhaven, USA). Using a 20Hz Pilatus 300K detector as SAXS detector (located at 3.4m from the sample) and a custom-designed Photonic Science CCD detector as WAXS detector (located at 20cm from the sample). Together, these two detectors cover a combined s range of 0.08 -19.600 nm-1, with overlapping data between 1.2 and 2.0 nm-1 (s = 4π sin θ/λ, where 2θ is the scattering angle). One solute concentration of 12.20 mg/ml was measured. Six successive 30 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the different curves were scaled for protein concentration. The low angle data collected were obtained from a single concentration scattering curve. For SAXS data analysis (RG, Pr function) we used the data up to 2.0 nm-1.

Cell temperature = UNKNOWN

Tags: waxs
Pseudomonas aeruginosa SDS hydrolase SdsA1
Mol. type   Protein
Organism   Pseudomonas aeruginosa
Olig. state   Dimer
Mon. MW   72.6 kDa
 
UniProt   Q9I5I9
Sequence   FASTA