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24 hits found for Siliqi

SASDKQ4 – EspK secretion protein - full length

ESX-1 secretion-associated protein EspK experimental SAS data
CORAL model
Sample: ESX-1 secretion-associated protein EspK monomer, 27 kDa Mycobacterium tuberculosis (strain … protein
Buffer: 20 mM Tris-HCl pH, 300 mM NaCl, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Sep 20
Structural Analysis of the Partially Disordered Protein EspK from Mycobacterium Tuberculosis Crystals 11(1):18 (2020)
...Siliqi D
RgGuinier 7.2 nm
Dmax 3.3 nm
VolumePorod 330 nm3

SASDKR4 – EspK secretion protein - C-terminal domain

ESX-1 secretion-associated protein EspK experimental SAS data
GASBOR model
Sample: ESX-1 secretion-associated protein EspK monomer, 30 kDa Mycobacterium tuberculosis (strain … protein
Buffer: 20 mM Tris-HCl pH, 300 mM NaCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Apr 12
Structural Analysis of the Partially Disordered Protein EspK from Mycobacterium Tuberculosis Crystals 11(1):18 (2020)
...Siliqi D
RgGuinier 2.2 nm
Dmax 8.4 nm
VolumePorod 52 nm3

SASDKF6 – C2AB construct of synaptotagmin-1 (SYT1) with E395-397A mutations at the linker

Synaptotagmin-1 experimental SAS data
CORAL model
Sample: Synaptotagmin-1 monomer, 33 kDa Arabidopsis thaliana protein
Buffer: 50 mM Tris, 50 mM NaCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Jun 11
The structure and flexibility analysis of the Arabidopsis synaptotagmin 1 reveal the basis of its regulation at membrane contact sites Life Science Alliance 4(10):e202101152 (2021)
...Siliqi D, Infantes L, Lagartera L, Mills A, Gago F, Ruiz-López N, Botella M, Sánchez-Barrena M, Albert A
RgGuinier 3.1 nm
Dmax 12.1 nm
VolumePorod 59 nm3

SASDKG6 – SMP2C2A construct of synaptotagmin-1 (SYT1)

Synaptotagmin-1 (SYT1-SMP2C2A) experimental SAS data
MULTIFOXS model
Sample: Synaptotagmin-1 (SYT1-SMP2C2A) monomer, 72 kDa Escherichia coli protein
Buffer: 20 mM Tris, 100 mM NaCl, 5% glycerol, 2 mM DTT, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Jun 11
The structure and flexibility analysis of the Arabidopsis synaptotagmin 1 reveal the basis of its regulation at membrane contact sites Life Science Alliance 4(10):e202101152 (2021)
...Siliqi D, Infantes L, Lagartera L, Mills A, Gago F, Ruiz-López N, Botella M, Sánchez-Barrena M, Albert A
RgGuinier 4.2 nm
Dmax 17.8 nm
VolumePorod 138 nm3

SASDAC8 – SDS hydrolase SdsA1

Pseudomonas aeruginosa SDS hydrolase SdsA1 experimental SAS data
CRYSOL model
Sample: Pseudomonas aeruginosa SDS hydrolase SdsA1 dimer, 145 kDa Pseudomonas aeruginosa protein
Buffer: 50 mM HEPES, pH: 7
Experiment: SAXS data collected at X9A, National Synchrotron Light Source (NSLS) on 2013 Jul 22
SdsA polymorph isolation and improvement of their crystal quality using nonconventional crystallization techniques Journal of Applied Crystallography 48(5):1551-1559 (2015)
...Siliqi D, Sánchez-Puig N, Moreno A
RgGuinier 3.6 nm
Dmax 16.9 nm
VolumePorod 261 nm3

SASDEN8 – Intracellular region of the human Cyclin and CBS domain divalent metal cation transport mediator-4 in complex with Phosphatase of Regenerating Liver-1 and MgATP

CNNM4_BAT-cNMP-CtailProtein tyrosine phosphatase type IVA 1 experimental SAS data
CORAL model
Sample: CNNM4_BAT-cNMP-Ctail dimer, 96 kDa Homo sapiens protein
Protein tyrosine phosphatase type IVA 1 dimer, 40 kDa Mus musculus protein
Buffer: HEPES buffer pH 7.4, 200 mM NaCl , 1mM DTT, pH: 7.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Sep 17
Structural Insights into the Intracellular Region of the Human Magnesium Transport Mediator CNNM4. Int J Mol Sci 20(24) (2019)
...Siliqi D, Martínez-Cruz LA
RgGuinier 4.4 nm
Dmax 18.3 nm
VolumePorod 216 nm3

SASDEP8 – Intracellular region of the human Cyclin and CBS domain divalent metal cation transport mediator-4 in complex with Phosphatase of Regenerating Liver-1

CNNM4_BAT-cNMP-CtailProtein tyrosine phosphatase type IVA 1 experimental SAS data
CORAL model
Sample: CNNM4_BAT-cNMP-Ctail dimer, 96 kDa Homo sapiens protein
Protein tyrosine phosphatase type IVA 1 dimer, 40 kDa Mus musculus protein
Buffer: HEPES buffer pH 7.4, 200 mM NaCl , 1mM DTT, pH: 7.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Aug 2
Structural Insights into the Intracellular Region of the Human Magnesium Transport Mediator CNNM4. Int J Mol Sci 20(24) (2019)
...Siliqi D, Martínez-Cruz LA
RgGuinier 4.7 nm
Dmax 18.5 nm
VolumePorod 226 nm3

SASDEQ8 – Intracellular region of the human Cyclin and CBS domain divalent metal cation transport mediator-4

CNNM4_BAT-cNMP-Ctail experimental SAS data
CORAL model
Sample: CNNM4_BAT-cNMP-Ctail dimer, 96 kDa Homo sapiens protein
Buffer: HEPES buffer pH 7.4, 200 mM NaCl , 1mM DTT, pH: 7.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Aug 2
Structural Insights into the Intracellular Region of the Human Magnesium Transport Mediator CNNM4. Int J Mol Sci 20(24) (2019)
...Siliqi D, Martínez-Cruz LA
RgGuinier 4.0 nm
Dmax 16.6 nm
VolumePorod 156 nm3

SASDER8 – Cyclic nucleotide homology domain of the human Cyclin and CBS domain divalent metal cation transport mediator-4

CNNM4_cNMP experimental SAS data
GASBOR model
Sample: CNNM4_cNMP dimer, 42 kDa Homo sapiens protein
Buffer: HEPES buffer pH 7.4, 200 mM NaCl , 1mM DTT, pH: 7.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Feb 7
Structural Insights into the Intracellular Region of the Human Magnesium Transport Mediator CNNM4. Int J Mol Sci 20(24) (2019)
...Siliqi D, Martínez-Cruz LA
RgGuinier 2.6 nm
Dmax 9.7 nm
VolumePorod 102 nm3

SASDES8 – Intracellular region of the human Cyclin and CBS domain divalent metal cation transport mediator-4 in complex with MgATP

CNNM4_BAT-cNMP-Ctail experimental SAS data
CORAL model
Sample: CNNM4_BAT-cNMP-Ctail dimer, 96 kDa Homo sapiens protein
Buffer: HEPES buffer pH 7.4, 200 mM NaCl , 1mM DTT, pH: 7.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Sep 17
Structural Insights into the Intracellular Region of the Human Magnesium Transport Mediator CNNM4. Int J Mol Sci 20(24) (2019)
...Siliqi D, Martínez-Cruz LA
RgGuinier 3.9 nm
Dmax 14.3 nm
VolumePorod 149 nm3

SASDKJ9 – C2AB construct of synaptotagmin-1 (SYT1)

Synaptotagmin-1 experimental SAS data
DAMFILT model
Sample: Synaptotagmin-1 monomer, 33 kDa Arabidopsis thaliana protein
Buffer: 50 mM Tris, 50 mM NaCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Feb 16
The structure and flexibility analysis of the Arabidopsis synaptotagmin 1 reveal the basis of its regulation at membrane contact sites Life Science Alliance 4(10):e202101152 (2021)
...Siliqi D, Infantes L, Lagartera L, Mills A, Gago F, Ruiz-López N, Botella M, Sánchez-Barrena M, Albert A
RgGuinier 2.8 nm
Dmax 12.6 nm
VolumePorod 50 nm3

SASDKK9 – C2AB construct of synaptotagmin-1 (SYT1) in presence of Ca cations

Synaptotagmin-1 experimental SAS data
MULTIFOXS model
Sample: Synaptotagmin-1 monomer, 33 kDa Arabidopsis thaliana protein
Buffer: 50 mM Tris, 50 mM NaCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Feb 16
The structure and flexibility analysis of the Arabidopsis synaptotagmin 1 reveal the basis of its regulation at membrane contact sites Life Science Alliance 4(10):e202101152 (2021)
...Siliqi D, Infantes L, Lagartera L, Mills A, Gago F, Ruiz-López N, Botella M, Sánchez-Barrena M, Albert A
RgGuinier 2.9 nm
Dmax 13.8 nm
VolumePorod 54 nm3

SASDDE7 – Class I chitinase 1 from Agave tequilana

Chitinase 1 experimental SAS data
DAMMIF model
Sample: Chitinase 1 monomer, 32 kDa Agave tequilana protein
Buffer: MES 50 mM, pH: 6
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2017 Apr 26
A biophysical and structural study of two chitinases from Agave tequilana and their potential role as defense proteins. FEBS J (2019)
...Siliqi D, Rodríguez-Romero A
RgGuinier 2.6 nm
Dmax 9.7 nm
VolumePorod 52 nm3

SASDMP7 – Apo-serotransferrin at pH 8.0

Serotransferrin experimental SAS data
GASBOR model
Sample: Serotransferrin monomer, 77 kDa Homo sapiens protein
Buffer: 15 mM HEPES, 20 mM NaHCO3, 50 mM NaCl (Buffer APO-Tf-1), pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2021 Apr 7
X-ray Characterization of Conformational Changes of Human Apo- and Holo-Transferrin International Journal of Molecular Sciences 22(24):13392 (2021)
...Siliqi D, Gonzalez-Ramirez L, Lopez-Sanchez C, Gavira J, Moreno A
RgGuinier 3.1 nm
Dmax 9.4 nm
VolumePorod 106 nm3

SASDEW8 – Shwachman-Bodian-Diamond Syndrome protein (SDO1) with domains 2 and 3

Ribosome maturation protein SDO1 experimental SAS data
DAMFILT model
Sample: Ribosome maturation protein SDO1 monomer, 17 kDa Saccharomyces cerevisiae protein
Buffer: 50 mM Tris pH 8.0, 10% glycerol, 300 mM NaCl and 5 mM MgCl2., pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Aug 14
Interaction of the GTPase Elongation Factor Like-1 with the Shwachman-Diamond Syndrome Protein and Its Missense Mutations. Int J Mol Sci 19(12) (2018)
...Siliqi D, Sánchez-Puig N
RgGuinier 2.1 nm
Dmax 6.2 nm
VolumePorod 25 nm3

SASDDA6 – Class I chitinase 2 from Agave tequilana

Chitinase 2 experimental SAS data
DAMMIF model
Sample: Chitinase 2 monomer, 32 kDa Agave tequilana protein
Buffer: MES 50 mM, pH: 6
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2017 Apr 19
A biophysical and structural study of two chitinases from Agave tequilana and their potential role as defense proteins. FEBS J (2019)
...Siliqi D, Rodríguez-Romero A
RgGuinier 2.4 nm
Dmax 9.8 nm
VolumePorod 49 nm3

SASDMN7 – Apo-serotransferrin at pH 7.0

Serotransferrin experimental SAS data
GASBOR model
Sample: Serotransferrin monomer, 77 kDa Homo sapiens protein
Buffer: 15 mM HEPES, 20 mM NaHCO3, 50 mM NaCl (APO Buffer), pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2021 Apr 7
X-ray Characterization of Conformational Changes of Human Apo- and Holo-Transferrin International Journal of Molecular Sciences 22(24):13392 (2021)
...Siliqi D, Gonzalez-Ramirez L, Lopez-Sanchez C, Gavira J, Moreno A
RgGuinier 3.1 nm
Dmax 9.6 nm
VolumePorod 106 nm3

SASDEV8 – Shwachman-Bodian-Diamond Syndrome protein (yeast SDO1)

Ribosome maturation protein SDO1 experimental SAS data
MULTIFOXS model
Sample: Ribosome maturation protein SDO1 monomer, 28 kDa Saccharomyces cerevisiae protein
Buffer: 50 mM Tris pH 8.0, 10% glycerol, 300 mM NaCl and 5 mM MgCl2., pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Sep 21
Interaction of the GTPase Elongation Factor Like-1 with the Shwachman-Diamond Syndrome Protein and Its Missense Mutations. Int J Mol Sci 19(12) (2018)
...Siliqi D, Sánchez-Puig N
RgGuinier 2.7 nm
Dmax 8.5 nm

SASDMM7 – Apo-serotransferrin at pH 5.5

Serotransferrin experimental SAS data
GASBOR model
Sample: Serotransferrin monomer, 77 kDa Homo sapiens protein
Buffer: 15 mM HEPES, 20 mM NaHCO3, 50 mM NaCl, (APO Buffer), pH: 5.5
Experiment: SAXS data collected at BM29, ESRF on 2021 Apr 7
X-ray Characterization of Conformational Changes of Human Apo- and Holo-Transferrin International Journal of Molecular Sciences 22(24):13392 (2021)
...Siliqi D, Gonzalez-Ramirez L, Lopez-Sanchez C, Gavira J, Moreno A
RgGuinier 3.1 nm
Dmax 9.8 nm
VolumePorod 106 nm3

SASDEU8 – GTPase Elongation Factor like-1 protein (yeast EFL1)

Ribosome assembly protein 1 experimental SAS data
DAMFILT model
Sample: Ribosome assembly protein 1 monomer, 124 kDa Saccharomyces cerevisiae protein
Buffer: 50 mM Tris pH 8.0, 10% glycerol, 300 mM NaCl and 5 mM MgCl2., pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Sep 21
Interaction of the GTPase Elongation Factor Like-1 with the Shwachman-Diamond Syndrome Protein and Its Missense Mutations. Int J Mol Sci 19(12) (2018)
...Siliqi D, Sánchez-Puig N
RgGuinier 4.7 nm
Dmax 15.8 nm
VolumePorod 258 nm3

SASDMS7 – HOLO-serotransferrin at pH 8.0 in the presence of iron

Serotransferrin experimental SAS data
GASBOR model
Sample: Serotransferrin monomer, 77 kDa Homo sapiens protein
Buffer: 15 mM HEPES, 20 mM NaHCO3, 50 mM NaCl (Buffer APO-Tf-1), pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2021 Apr 7
X-ray Characterization of Conformational Changes of Human Apo- and Holo-Transferrin International Journal of Molecular Sciences 22(24):13392 (2021)
...Siliqi D, Gonzalez-Ramirez L, Lopez-Sanchez C, Gavira J, Moreno A
RgGuinier 3.2 nm
Dmax 13.1 nm
VolumePorod 106 nm3

SASDET8 – GTPase Elongation Factor like-1 bound Shwachman-Diamond Syndrome protein (EFL1*SDo1)

Ribosome assembly protein 1Ribosome maturation protein SDO1 experimental SAS data
DAMFILT model
Sample: Ribosome assembly protein 1 monomer, 124 kDa Saccharomyces cerevisiae protein
Ribosome maturation protein SDO1 monomer, 28 kDa Saccharomyces cerevisiae protein
Buffer: 50 mM Tris pH 8.0, 10% glycerol, 300 mM NaCl and 5 mM MgCl2., pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Sep 21
Interaction of the GTPase Elongation Factor Like-1 with the Shwachman-Diamond Syndrome Protein and Its Missense Mutations. Int J Mol Sci 19(12) (2018)
...Siliqi D, Sánchez-Puig N
RgGuinier 5.0 nm
Dmax 16.0 nm
VolumePorod 333 nm3

SASDMR7 – HOLO-serotransferrin at pH 7.0 in the presence of iron

Serotransferrin experimental SAS data
GASBOR model
Sample: Serotransferrin monomer, 77 kDa Homo sapiens protein
Buffer: 15 mM HEPES, 20 mM NaHCO3, 50 mM NaCl (APO Buffer), pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2021 Apr 7
X-ray Characterization of Conformational Changes of Human Apo- and Holo-Transferrin International Journal of Molecular Sciences 22(24):13392 (2021)
...Siliqi D, Gonzalez-Ramirez L, Lopez-Sanchez C, Gavira J, Moreno A
RgGuinier 3.2 nm
Dmax 13.5 nm
VolumePorod 103 nm3

SASDMQ7 – HOLO-serotransferrin at pH 5.5 in the presence of iron

Serotransferrin experimental SAS data
GASBOR model
Sample: Serotransferrin monomer, 77 kDa Homo sapiens protein
Buffer: 15 mM HEPES, 20 mM NaHCO3, 50 mM NaCl, (APO Buffer), pH: 5.5
Experiment: SAXS data collected at BM29, ESRF on 2021 Apr 7
X-ray Characterization of Conformational Changes of Human Apo- and Holo-Transferrin International Journal of Molecular Sciences 22(24):13392 (2021)
...Siliqi D, Gonzalez-Ramirez L, Lopez-Sanchez C, Gavira J, Moreno A
RgGuinier 3.3 nm
Dmax 14.6 nm
VolumePorod 107 nm3