Standard proteins

Darja Ruskule.

SASDAG2 – Lysozyme in sodium acetate

Lysozyme C
MWI(0) 11 kDa
MWexpected 14 kDa
VPorod 21 nm3
log I(s) 1.22×101 1.22×100 1.22×10-1 1.22×10-2
Lysozyme C small angle scattering data  s, nm-1
ln I(s)
Lysozyme C Guinier plot ln 1.23×101 Rg: 1.5 nm 0 (1.5 nm)-2 s2
Lysozyme C Kratky plot 1.104 0 3 sRg
Lysozyme C pair distance distribution function Rg: 1.4 nm 0 Dmax: 4 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Lysozyme C GASBOR model

log I(s)
 s, nm-1
Lysozyme C PDB (PROTEIN DATA BANK) model

Synchrotron SAXS data from solutions of Lysozyme in sodium acetate in 40 mM Sodium Acetate, pH 3.8 were collected on the EMBL X33 beam line at the DORIS III storage ring (Hamburg, Germany) using a Pilatus 1M-W detector at a sample-detector distance of 2.7 m and at a wavelength of λ = 0.15 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 8.09 mg/ml was measured at 10°C. Eight successive 15 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.

Concentration min = UNKNOWN

Tags: waxs X33
Lysozyme C (Lys)
Mol. type   Protein
Organism   Gallus gallus
Olig. state   Monomer
Mon. MW   16.2 kDa
UniProt   P00698
Sequence   FASTA