Standard proteins

Darja Ruskule.

SASDAL2 – Ovalbumin in PBS

Ovalbumin
MWI(0) 36 kDa
MWexpected 43 kDa
VPorod 74 nm3
log I(s) 3.70×101 3.70×100 3.70×10-1 3.70×10-2
Ovalbumin small angle scattering data  s, nm-1
ln I(s)
Ovalbumin Guinier plot ln 3.70×101 Rg: 2.5 nm 0 (2.5 nm)-2 s2
(sRg)2I(s)/I(0)
Ovalbumin Kratky plot 1.104 0 3 sRg
p(r)
Ovalbumin pair distance distribution function Rg: 2.4 nm 0 Dmax: 7.8 nm

Data validation


Fits and models


log I(s)
 s, nm-1
Ovalbumin DAMMIN model

log I(s)
 s, nm-1
Ovalbumin PDB (PROTEIN DATA BANK) model

Synchrotron SAXS data from solutions of Ovalbumin in PBS in PBS, pH 7.4 were collected on the EMBL X33 beam line at the DORIS III storage ring (Hamburg, Germany) using a Pilatus 1M-W detector at a sample-detector distance of 2.7 m and at a wavelength of λ = 0.15 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 24.20 mg/ml was measured at 10°C. Eight successive 15 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Tags: X33
Ovalbumin
Mol. type   Protein
Organism   Gallus gallus
Olig. state   Monomer
Mon. MW   42.9 kDa
 
UniProt   P01012
Sequence   FASTA
 
PDB ID   1OVA