The CD27L and CTP1L endolysins targeting Clostridia contain a built-in trigger and release factor.

Dunne M, Mertens HD, Garefalaki V, Jeffries CM, Thompson A, Lemke EA, Svergun DI, Mayer MJ, Narbad A, Meijers R, PLoS Pathog 10(7):e1004228 (2014) Europe PMC

SASDAL5 – Clostridium difficile bacteriophage 27 endolysin dimer, CD27L

Clostridium difficile bacteriophage 27 endolysin
MWI(0) 42 kDa
MWexpected 64 kDa
VPorod 72 nm3
log I(s) 4.56×101 4.56×100 4.56×10-1 4.56×10-2
Clostridium difficile bacteriophage 27 endolysin small angle scattering data  s, nm-1
ln I(s)
Clostridium difficile bacteriophage 27 endolysin Guinier plot ln 4.56×101 Rg: 3.3 nm 0 (3.3 nm)-2 s2
(sRg)2I(s)/I(0)
Clostridium difficile bacteriophage 27 endolysin Kratky plot 1.104 0 3 sRg
p(r)
Clostridium difficile bacteriophage 27 endolysin pair distance distribution function Rg: 3.3 nm 0 Dmax: 10.6 nm

Data validation


Fits and models


log I(s)
 s, nm-1
Clostridium difficile bacteriophage 27 endolysin NONE model
Clostridium difficile bacteriophage 27 endolysin NONE model
Clostridium difficile bacteriophage 27 endolysin NONE model
Clostridium difficile bacteriophage 27 endolysin NONE model
Clostridium difficile bacteriophage 27 endolysin NONE model
Clostridium difficile bacteriophage 27 endolysin NONE model
Clostridium difficile bacteriophage 27 endolysin NONE model
Clostridium difficile bacteriophage 27 endolysin NONE model

Synchrotron SAXS data from solutions of Clostridium difficile bacteriophage 27 endolysin dimer, CD27L in 20 mM HEPES 150 mM NaCl, pH 7.5 were collected on the EMBL X33 beam line at the DORIS III storage ring (Hamburg, Germany) using a Pilatus 1M-W detector at a sample-detector distance of 2.7 m and at a wavelength of λ = 0.15 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 0.9 and 4 mg/ml were measured at 10°C. Eight successive 15 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.

Tags: X33
Clostridium difficile bacteriophage 27 endolysin (CD27L)
Mol. type   Protein
Organism   Clostridioides difficile
Olig. state   Dimer
Mon. MW   32.1 kDa
Sequence   FASTA