Standard proteins

Darja Ruskule.

SASDAN2 – Bovine pancreatic ribonuclease A in PBS (WAXS)

Ribonuclease pancreatic
MWI(0) 15 kDa
MWexpected 16 kDa
VPorod 15 nm3
log I(s) 1.35×101 1.35×100 1.35×10-1 1.35×10-2
Ribonuclease pancreatic small angle scattering data  s, nm-1
ln I(s)
Ribonuclease pancreatic Guinier plot ln 1.35×101 Rg: 1.6 nm 0 (1.6 nm)-2 s2
(sRg)2I(s)/I(0)
Ribonuclease pancreatic Kratky plot 1.104 0 3 sRg
p(r)
Ribonuclease pancreatic pair distance distribution function Rg: 1.6 nm 0 Dmax: 5 nm

Data validation


Fits and models


log I(s)
 s, nm-1
Ribonuclease pancreatic GASBOR model

log I(s)
 s, nm-1
Ribonuclease pancreatic PDB (PROTEIN DATA BANK) model

Synchrotron SAXS data from solutions of Bovine pancreatic ribonuclease A in PBS (WAXS) in PBS, pH 7.4 were collected on the EMBL X33 beam line at the DORIS III storage ring (Hamburg, Germany) using a Pilatus 1M-W detector at a sample-detector distance of 2.7 m and at a wavelength of λ = 0.15 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 22.60 mg/ml was measured at 10°C. Eight successive 15 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.

Merged

Tags: waxs X33
Ribonuclease pancreatic (RNase)
Mol. type   Protein
Organism   Bos taurus
Olig. state   Monomer
Mon. MW   16.5 kDa
 
UniProt   P61823
Sequence   FASTA
 
PDB ID   1C0B