Standard proteins

Darja Ruskule.

SASDAN2 – RNase in PBS

Ribonuclease pancreatic
MWI(0) 15 kDa
MWexpected 16 kDa
VPorod 15 nm3
log I(s) 1.35×101 1.35×100 1.35×10-1 1.35×10-2
Ribonuclease pancreatic small angle scattering data  s, nm-1
ln I(s)
Ribonuclease pancreatic Guinier plot ln 1.35×101 Rg: 1.6 nm 0 (1.6 nm)-2 s2
(sRg)2I(s)/I(0)
Ribonuclease pancreatic Kratky plot 1.104 0 3 sRg
p(r)
Ribonuclease pancreatic pair distance distribution function Rg: 1.6 nm 0 Dmax: 5 nm

Data validation


Fits and models


log I(s)
 s, nm-1
Ribonuclease pancreatic GASBOR model

log I(s)
 s, nm-1
Ribonuclease pancreatic PDB (PROTEIN DATA BANK) model

Synchrotron SAXS data from solutions of RNase in PBS in PBS, pH 7.4 were collected on the EMBL X33 beam line at the DORIS III storage ring (Hamburg, Germany) using a Pilatus 1M-W detector at a sample-detector distance of 2.7 m and at a wavelength of λ = 0.15 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 22.60 mg/ml was measured at 10°C. Eight successive 15 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.

Merged

Ribonuclease pancreatic (RNase)
Mol. type   Protein
Organism   Bos taurus
Olig. state   Monomer
Mon. MW   16.5 kDa
 
UniProt   P61823
Sequence   FASTA
 
PDB ID   1C0B