| MWI(0) | 77 | kDa |
| MWexpected | 73 | kDa |
| VPorod | 143 | nm3 |
|
log I(s)
1.58×103
1.58×102
1.58×101
1.58×100
|
s, nm-1
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The flexibility of a homeodomain transcription factor heterodimer and its allosteric regulation by DNA binding.
Mathiasen L, Valentini E, Boivin S, Cattaneo A, Blasi F, Svergun DI, Bruckmann C, FEBS J 283(16):3134-54 (2016) Europe PMCSASDAP7 – PBX1-PREB1 complex
Pre-B-cell leukemia transcription factor 1Homeobox protein PKNOX1
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Fits and models
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Synchrotron SAXS
data from solutions of
PBX1-PREB1 complex
in
20 mM Tris-HCl 150 mM NaCl 5% glycerol 1 mM DTT, pH 7.4
were collected
on the
EMBL P12 beam line
at the PETRA III storage ring
(DESY; Hamburg, Germany)
using a Pilatus 2M detector
at a sample-detector distance of 3.1 m and
at a wavelength of λ = 0.12 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
Solute concentrations ranging between 1.5 and 2.8 mg/ml were measured
.
50 successive
0.050 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.
Cell temperature = UNKNOWN. Storage temperature = UNKNOWN |
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