The flexibility of a homeodomain transcription factor heterodimer and its allosteric regulation by DNA binding.

Mathiasen L, Valentini E, Boivin S, Cattaneo A, Blasi F, Svergun DI, Bruckmann C
FEBS J 283(16):3134-54 (2016 Aug)

PMID: 27390177 doi: 10.1111/febs.13801

Published in SASBDB: 2016 Jul 25

SASDAP7 – PBX1-PREB1 complex Sample: Pre-B-cell leukemia transcription factor 1 monomer, 34 kDa Homo sapiens protein Homeobox protein PKNOX1 monomer, 38 kDa Homo sapiens protein Buffer: 20 mM Tris-HCl 150 mM NaCl 5% glycerol 1 mM DTT, pH: 7.4 Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Dec 6 RgGuinier 5.8 nm Dmax 19.0 nm VolumePorod 143 nm3

SASDAQ7 – PBX1-PREB1-DNA complex Sample: Pre-B-cell leukemia transcription factor 1 monomer, 34 kDa Homo sapiens protein Homeobox protein PKNOX1 monomer, 38 kDa Homo sapiens protein DNA 22mer monomer, 12 kDa DNA Buffer: 20 mM Tris-HCl 150 mM NaCl 5% glycerol 1 mM DTT, pH: 7.4 Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Dec 6 RgGuinier 4.8 nm Dmax 16.3 nm VolumePorod 173 nm3

SASDAR7 – DNA 22mer Sample: DNA 22mer monomer, 12 kDa DNA Buffer: 20 mM Tris-HCl 150 mM NaCl 5% glycerol 1 mM DTT, pH: 7.4 Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Dec 6 RgGuinier 2.2 nm Dmax 7.5 nm VolumePorod 19 nm3